), despite incorporating results from over 30 individual laboratory, theoretical, and field studies since the 1970s. Airborne and ground-based observations from the Deep Convective Clouds and Chemistry experiment in May and June 2012 provide a new data set for calculating moles of NO x produced per lightning flash, P(NO x ), in thunderstorms over the United States Great Plains. This analysis utilizes a combination of in situ observations of storm inflow and outflow from three instrumented aircraft, three-dimensional spatial information from ground-based radars and satellite observations, and spatial and temporal information for intracloud and cloud-to-ground lightning flashes from ground-based lightning mapping arrays. Evaluation of two analysis methods (e.g., a volume-based approach and a flux-based approach) for converting enhancements in lightning-produced NO x from volume-based mixing ratios to moles NO x flash À1 suggests that both methods equally approximate P(NO x ) for storms with elongated anvils, while the volume-based approach better approximates P(NO x ) for storms with circular-shaped anvils. Results from the more robust volume-based approach for three storms sampled over ), results from this analysis of DC3 storms offer more constrained upper and lower limits for P(NO x ) in this geographical region.