Lightwave-reinforced stem cells with enhanced wound healing efficacy

Abstract

Comprehensive research has led to significant preclinical outcomes in modified human adipose-derived mesenchymal stem cells (hADSCs). Photobiomodulation (PBM), a technique to enhance the cellular capacity of stem cells, has attracted considerable attention owing to its effectiveness and safety. Here, we suggest a red organic light-emitting diode (OLED)-based PBM strategy to augment the therapeutic efficacy of hADSCs. In vitro assessments revealed that hADSCs basked in red OLED light exhibited enhanced angiogenesis, cell adhesion, and migration compared to naïve hADSCs. We demonstrated that the enhancement of cellular capacity was due to an increased level of intracellular reactive oxygen species. Furthermore, accelerated healing and regulated inflammatory response was observed in mice transplanted with red light-basked hADSCs. Overall, our findings suggest that OLED-based PBM may be an easily accessible and attractive approach for tissue regeneration that can be applied to various clinical stem cell therapies.

Could Light‐Based Technologies Improve Stem Cell Therapy for Skin Wounds? A Systematic Review and Meta‐Analysis of Preclinical Studies†

“…As a result, 14 articles were assessed for eligibility, resulting in nine articles that were included in this review. Five studies were disqualified because they did not fit our inclusion criteria, that is, three showed absent and/or unreliable light protocols (14–16), one did not report surface markers or differentiation assays for SCs (17) and one did not present nonirradiated SC group as a control group (18) (Fig. 1).…”

Exploring the Safety and Efficacy of Organic Light-Emitting Diode in Skin Rejuvenation and Wound Healing

“…Previous studies on the wound healing effect of LED have demonstrated that LED stimulates the proliferation and migration of various types of cells while promoting angiogenesis, the expression of growth factors, and the production of collagen, thereby assisting the process of wound healing. 11 21 22 23 24 Recent studies have also applied OLEDs and observed improved cellular function and wound healing in vivo. 11 15 We performed cell scratch wound healing assay in vitro and observed that the migration ability of HDFs was significantly increased after exposure to 6 J/cm 2 OLED and LED, similar to the previous report on the positive effect of OLED on the fibroblast proliferation.…”

“… 11 21 22 23 24 Recent studies have also applied OLEDs and observed improved cellular function and wound healing in vivo. 11 15 We performed cell scratch wound healing assay in vitro and observed that the migration ability of HDFs was significantly increased after exposure to 6 J/cm 2 OLED and LED, similar to the previous report on the positive effect of OLED on the fibroblast proliferation. 25 Additionally, our group performed in vivo study with animal mouse model to investigate skin rejuvenation effect of OLEDs on UV-exposed skin.…”

“… 9 10 Unlike LEDs, OLEDs are surface light sources that can uniformly distribute light over a wide area. 11 Moreover, OLED is a useful source for PBM due to their safety, high efficiency, low power consumption, and lack of overheating. As OLED devices are flexible and lightweight, they can be easily transformed into wearable units, making it easier to utilize them as daily cosmeceutical devices compared to LED devices.…”

Stem Cells and Angiogenesis: Implications and Limitations in Enhancing Chronic Diabetic Foot Ulcer Healing

“…Administering stem cells with a biomaterial carrier may mitigate the limitations associated with the number and viability of stem cells but needs extensive research [ 19 , 20 ]. Administration of preconditioned stem cells with photo-biomodulation is another strategy to enhance the viability and activity of stem cells to enhance angiogenesis and wound healing [ 68 , 69 , 70 ]. With the background of the complications associated with autologous MSCs, Palma et al [ 71 ] reported that one specific subpopulation derived from the Wharton’s jelly of the umbilical cord (ucMSC), the differentiated mesenchymal cells (DMCs), has the potential of improving wound healing with increased angiogenesis without any rejection of the transplanted cells in immunocompetent mice.…”