Ligilactobacillus salivarius functionalities, applications, and manufacturing challenges

Sanchez, Maria Guerrero; Passot, Stéphanie; Campoy, Sonia; Olivares, Mónica; Fonseca, Fernanda

doi:10.1007/s00253-021-11694-0

Cited by 53 publications

(39 citation statements)

References 174 publications

(184 reference statements)

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“…Several studies in the last decade report probiotic properties of different L. salivarius strains such as antimicrobial activity, immunological effects, and modulation of the intestinal and vaginal microbiota (Guerrero Sanchez et al 2022). Their incorporation as probiotics in food supplements and medicines requires obtained a powder, and thus, in turn, a cascade of operations, ranging from fermentation to stabilization (by freeze-drying or spray-drying) and final storage at room temperature (Fenster et al 2019).…”

Section: Introductionmentioning

confidence: 99%

Effect of protective agents on the storage stability of freeze-dried Ligilactobacillus salivarius CECT5713

Sanchez

Passot

Campoy

et al. 2022

Appl Microbiol Biotechnol

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Ligilactobacillus salivarius is a lactic acid bacterium exhibiting several health benefits but remains commercially underexploited due to its inability to survive during long-term storage in the dried state. Our objective was to study the effect of various protective molecules (maltodextrin, trehalose, antioxidants, and fructooligosaccharides), being efficient on other bacteria, on the freeze-dried stability of L. salivarius CECT5713. The culturability was evaluated after freezing, freeze-drying, and subsequent storage at 37 °C, as well as the biochemical composition of cells in an aqueous environment using Fourier Transform Infrared (FTIR) micro-spectroscopy. The assignment of principal absorption bands to cellular components was performed using data from the literature on bacteria. The membrane fatty acid composition was determined after freeze-drying and storage. Glass transition temperature of the liquid and freeze-dried bacterial suspensions and water activity of the freeze-dried samples were measured. The best storage stability was observed for the formulations involving maltodextrin and antioxidants. The analysis of the FTIR spectra of freeze-thawed cells and rehydrated cells after freeze-drying and storage revealed that freeze-drying induced damage to proteins, peptidoglycans of the cell wall and nucleic acids. Storage stability appeared to be dependent on the ability of the protective molecules to limit damage during freeze-drying. The inactivation rates of bacteria during storage were analyzed as a function of the temperature difference between the product temperature during sublimation or during storage and the glass transition temperature, allowing a better insight into the stabilization mechanisms of freeze-dried bacteria. Maintaining during the process a product temperature well below the glass transition temperature, especially during storage, appeared essential for L. salivarius CECT5713 storage stability. Key points L. salivarius CECT5713 highly resisted freezing but was sensitive to freeze-drying and storage  Freeze-drying and storage mainly altered cell proteins, peptidoglycans and nucleic acids  A glassy matrix containing maltodextrin and an antioxidant ensured the highest storage stability

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Section: Introductionmentioning

confidence: 99%

Effect of protective agents on the storage stability of freeze-dried Ligilactobacillus salivarius CECT5713

Sanchez

Passot

Campoy

et al. 2022

Appl Microbiol Biotechnol

View full text Add to dashboard Cite

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“…In contrast, high-dose RBM treatment resulted in higher quantities of the genera Ligilactobacillus and Duncaniella . As Ligilactobacillus has recently been gaining attention as a probiotic [ 33 ], our data raise the possibility that RBM may have prebiotic properties. Prior research suggests that Duncaniella , a butyrate-enriched species, contributes to colitis protection with its anti-inflammatory properties [ 34 ].…”

Section: Discussionmentioning

confidence: 70%

Dietary Resveratrol Butyrate Monoester Supplement Improves Hypertension and Kidney Dysfunction in a Young Rat Chronic Kidney Disease Model

et al. 2023

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Chronic kidney disease (CKD) remains a public health problem. Certain dietary supplements can assist in the prevention of CKD progression. In this regard, resveratrol is a polyphenol and has a potential therapeutic role in alleviating CKD. We previously utilized butyrate in order to improve the bioavailability of resveratrol via esterification and generated a resveratrol butyrate monoester (RBM). In this study, the hypothesis that RBM supplementation is able to protect against kidney dysfunction and hypertension was tested by using an adenine-induced CKD model. For this purpose, three-week-old male Sprague Dawley rats (n = 40) were equally categorized into: group 1—CN (sham control); group 2—CKD (adenine-fed rats); group 3—REV (CKD rats treated with 50 mg/L resveratrol); group 4—MEL (CKD rats treated with 25 mg/L RBM); and group 5—MEH (CKD rats treated with 50 mg/L RBM). At the end of a 12-week period, the rats were then euthanized. The adenine-fed rats displayed hypertension and kidney dysfunction, which were attenuated by dietary supplementation with RBM. The CKD-induced hypertension coincided with: decreased nitric oxide (NO) bioavailability; augmented renal protein expression of a (pro)renin receptor and angiotensin II type 1 receptor; and increased oxidative stress damage. Additionally, RBM and resveratrol supplementation shaped distinct gut microbiota profiles in the adenine-treated CKD rats. The positive effect of high-dose RBM was shown together with an increased abundance of the genera Duncaniella, Ligilactobacillus, and Monoglobus, as well as a decrease in Eubacterium and Schaedierella. Importantly, the mechanism of action of the RBM supplementation may be related to the restoration of NO, rebalancing of the RAS, a reduction in oxidative stress, and alterations to the gut microbiota. Moreover, RBM supplementation shows promise for the purposes of improving CKD outcomes and hypertension. As such, further translation to human studies is warranted.

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“…Both genera Ligilactobacillus and Tyzzerella belong to the phylum Firmicutes, although the impact of these taxa on the host health is different. Several stains of Ligilactobacillus sp belonging to the Lactobacillaceae family demonstrate probiotic properties [ 99 , 100 , 101 , 102 ]. In contrast, representatives of the genus Tyzzerella might be pathogenic [ 103 , 104 ] or associated with a high inflammatory background [ 105 , 106 ].…”

Section: Discussionmentioning

confidence: 99%

Gut Microbiota Alterations in Trace Amine-Associated Receptor 9 (TAAR9) Knockout Rats

et al. 2022

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Trace amine-associated receptors (TAAR1-TAAR9) are a family of G-protein-coupled monoaminergic receptors which might have great pharmacological potential. It has now been well established that TAAR1 plays an important role in the central nervous system. Interestingly, deletion of TAAR9 in rats leads to alterations in the periphery. Previously, we found that knockout of TAAR9 in rats (TAAR9-KO rats) decreased low-density lipoprotein cholesterol levels in the blood. TAAR9 was also identified in intestinal tissues, and it is known that it responds to polyamines. To elucidate the role of TAAR9 in the intestinal epithelium, we analyzed TAAR9-co-expressed gene clusters in public data for cecum samples. As identified by gene ontology enrichment analysis, in the intestine, TAAR9 is co-expressed with genes involved in intestinal mucosa homeostasis and function, including cell organization, differentiation, and death. Additionally, TAAR9 was co-expressed with genes implicated in dopamine signaling, which may suggest a role for this receptor in the regulation of peripheral dopaminergic transmission. To further investigate how TAAR9 might be involved in colonic mucosal homeostasis, we analyzed the fecal microbiome composition in TAAR9-KO rats and their wild-type littermates. We identified a significant difference in the number of observed taxa between the microbiome of TAAR9-KO and wild-type rats. In TAAR9-KO rats, the gut microbial community became more variable compared with the wild-type rats. Furthermore, it was found that the family Saccharimonadaceae, which is one of the top 10 most abundant families in TAAR9-KO rat feces, is almost completely absent in wild-type animal fecal samples. Taken together, these data indicate a role of TAAR9 in intestinal function.

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Ligilactobacillus salivarius functionalities, applications, and manufacturing challenges

Cited by 53 publications

References 174 publications

Effect of protective agents on the storage stability of freeze-dried Ligilactobacillus salivarius CECT5713

Effect of protective agents on the storage stability of freeze-dried Ligilactobacillus salivarius CECT5713

Dietary Resveratrol Butyrate Monoester Supplement Improves Hypertension and Kidney Dysfunction in a Young Rat Chronic Kidney Disease Model

Gut Microbiota Alterations in Trace Amine-Associated Receptor 9 (TAAR9) Knockout Rats

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