This paper compares the characteristics and properties of wood from trees grown on forest land with trees grown on former farmland. The first generation of the tree stand, which was artificially introduced on lands previously used for farming, was accepted as an ecosystem on the former farmland. A total of 36 trees from 12 areas were chosen for the comparisons, where six areas contained former farmland and six contained forest land. The compared tree stands differed from each other only in terms of the growth conditions, i.e., forest and former farmland soils. Selected properties and characteristics of the tree tissue, including density, bending strength, wood static compression, thickness of the cell wall, and lignin content in the dry mass, were subject to analysis. The conducted research found significant differences in the analyzed variables between the compared tree groups. The pines grown on former farmland soil were characterized by a generally poor technical wood quality, thinner tracheid walls, and lower lignin content.