Lignin binding to pancreatic lipase and its influence on enzymatic activity

Zhang, Juan; Xiao, Lin; Yang, Yucai; Wang, Zhaoxia; Li, Genxi

doi:10.1016/j.foodchem.2013.10.067

Cited by 51 publications

(30 citation statements)

References 35 publications

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“…The authors attributed the active effect of lignin to its rough and relatively well-developed surface, where numerous cavities ensure optimal distribution and stable contact between starch and the enzyme. Researchers observed that lignin-immobilized enzymes were characterized by higher catalytic efficiency and storage stability than their free counterparts [51,58,59]. The observations made in the earlier studies [25,26] and the results of the current microbiological and biochemical analyses lead to a similar conclusion.…”

Section: Bacterial Count and Enzymatic Activitymentioning

confidence: 71%

“…So far, other researchers have mainly focused on assessing the activation and stabilization of specific enzymes by lignin. For example, lignin was proved to activate α-amylase and lipase [58], where lipase could be immobilized on cellulose/lignin beads [59] or on the chitin/lignin composite [23]. Gong et al (2017) used lignin extracted from bamboo shoot shells (BBS) to activate and α-amylase immobilization [54].…”

Section: Process Performancementioning

confidence: 99%

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The Use of Lignin as a Microbial Carrier in the Co-Digestion of Cheese and Wafer Waste

et al. 2019

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The aim of the article was to present the effects of lignin grafted with polyvinylpyrrolidone (PVP) as a microbial carrier in anaerobic co-digestion (AcoD) of cheese (CE) and wafer waste (WF). Individual samples of waste cheese and wafers were also tested. The PVP modifier was used to improve the adhesive properties of the carrier surface. Lignin is a natural biopolymer which exhibits all the properties of a good carrier, including nontoxicity, biocompatibility, porosity, and thermal stability. Moreover, the analysis of the zeta potential of lignin and lignin combined with PVP showed their high electrokinetic stability within a wide pH range, that is, 4-11. The AcoD process was conducted under mesophilic conditions in a laboratory by means of anaerobic batch reactors. Monitoring with two standard parameters: pH and the VFA/TA ratio (volatile fatty acids-to-total alkalinity ratio) proved that the process was stable in all the samples tested. The high share of N-NH 4 + in TKN (total Kjeldahl nitrogen), which exceeded 90% for WF+CE and CE at the last phases of the process, proved the effective conversion of nitrogen forms. The microbiological analyses showed that eubacteria proliferated intensively and the dehydrogenase activity increased in the samples containing the carrier, especially in the system with two co-substrates (WF+CE/lignin) and in the waste cheese sample (CE/lignin). The biogas production increased from 1102.00 m 3 Mg −1 VS (volatile solids) to 1257.38 m 3 Mg −1 VS in the WF+CE/lignin sample, and from 881.26 m 3 Mg −1 VS to 989.65 m 3 Mg −1 VS in the CE/lignin sample. The research results showed that the cell immobilization on lignin had very positive effect on the anaerobic digestion process.

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Section: Bacterial Count and Enzymatic Activitymentioning

confidence: 71%

Section: Process Performancementioning

confidence: 99%

The Use of Lignin as a Microbial Carrier in the Co-Digestion of Cheese and Wafer Waste

et al. 2019

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“…Study on the binding interaction between active compounds and enzymes helps to understand the biochemical process of the active compounds [25]. Fluorescence quenching is a classical method to address such issue and has been widely used for probing the structure-affinity relationship in bindings of drugs to proteins [26,27].…”

Section: Introductionmentioning

confidence: 99%

Screening of lipase inhibitors from Scutellaria baicalensis extract using lipase immobilized on magnetic nanoparticles and study on the inhibitory mechanism

et al. 2016

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Scutellaria baicalensis is a traditional Chinese medicinal plant possessing a wide variety of biological activities. In this work, lipase immobilized on magnetic nanoparticles (LMNPs) was used as solid phase extract absorbent for screening of lipase inhibitors from this plant. Three flavonoids were found to bind to LMNPs and were identified as baicalin, wogonin, and oroxylin A by liquid chromatography-mass spectrometry (HPLC-MS). Their IC 50 values were determined to be 229.22 ± 12.67, 153.71 ± 9.21, and 56.07 ± 4.90 μM, respectively. Fluorescence spectroscopy and molecular docking were used to probe the interactions between these flavonoids and lipase. All the flavonoids quenched the fluorescence of lipase statically by forming new complexes, implying their affinities with the enzyme. The thermodynamic analysis suggested that van der Waals force and hydrogen bond were the main forces between wogonin and lipase, while hydrophobic force was the main force for the other two flavonoids. The results from a molecular docking study further revealed that all of them could insert into the pocket of lipase binding to a couple of amino acid residues.

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“…Pancreatic lipase is considered the principal lipolytic enzyme which plays an essential role in the efficient digestion of lipids and is responsible for the hydrolysis of about 70% of total ingested fats [53].…”

Section: Discussionmentioning

confidence: 99%

Investigation of the antiobesity and antioxidant properties of wild Plumbago europaea and Plumbago auriculata from North Palestine

Jaradat

Zaid

Hussein

2016

Chem. Biol. Technol. Agric.

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Background: Historically, plants have proven their value as a source of phytochemicals with therapeutic potentials and recently they play an important role in the discovery of novel drugs. Objectives:The current study aimed to investigate new antiobesity drugs from Plumbago europaea and Plumbago auriculata through the inhibition of the adsorption of dietary lipids. In vitro porcine pancreatic lipase inhibitory tests were conducted with weight reduction property as well as this study aimed to explore the antioxidant potential and to estimate total phenolic, flavonoid, and tannin contents in both of the studied species.Methods: Antioxidant capacity was evaluated using ferric reducing antioxidant power and DPPH assays, and porcine pancreatic lipase inhibitory tests were conducted using the UV spectrophotometric method, while total flavonoid, phenol, and tannin contents were estimated using standard phytochemical analytical methods.Results: Antioxidant potential and total flavonoid, phenol, and tannin contents of P. europaea were significantly higher than those of P. auriculata, and both of the studied species have potential antiobesity activity in comparison with orlistat. Conclusion:Results of the present study show that both of the studied Plumbago species consist of bioactive compounds that can act as lipase inhibitors and therefore can be useful for the development of functional foods against obesity. It can also be used as a source of lead compounds for designing new antiobesity drugs. Further isolation, identification, and characterization of phyto-active compounds responsible for antiobesity action are required to evaluate the full therapeutic potentials of these plants.

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Lignin binding to pancreatic lipase and its influence on enzymatic activity

Cited by 51 publications

References 35 publications

The Use of Lignin as a Microbial Carrier in the Co-Digestion of Cheese and Wafer Waste

The Use of Lignin as a Microbial Carrier in the Co-Digestion of Cheese and Wafer Waste

Screening of lipase inhibitors from Scutellaria baicalensis extract using lipase immobilized on magnetic nanoparticles and study on the inhibitory mechanism

Investigation of the antiobesity and antioxidant properties of wild Plumbago europaea and Plumbago auriculata from North Palestine

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