Lignin biodegradation and ligninolytic enzyme studies during biopulping of Acacia mangium wood chips by tropical white rot fungi

Liew, C. Y.; Husaini, Ahmad; Hussain, Hasnain; Muid, Sepiah; Liew, Kang Chiang; Roslan, Hairul Azman

doi:10.1007/s11274-010-0598-x

Cited by 50 publications

(22 citation statements)

References 23 publications

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“…as well as Phellinus sp. revealed the mechanism of lignin degradation and its association among ligninolytic enzymes activities formed by fungal cultures (Liew et al 2011). The enzymatic treatment of wood pulp with Aspergillus fumigates laccase mediator system showed rougher surface indicating the process of peeling with more exterior fibrillation (Vivekanand et al 2008).…”

Section: Sem Analysismentioning

confidence: 97%

Optimization of laccase production and its application in delignification of biomass

Bagewadi

2017

Int J Recycl Org Waste Agricult

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Purpose Current research focuses on the biological delignification of biomass by microbial laccase which is an environmentally friendly process. Methods Various statistical approaches were designed for optimization of laccase production like Plackett-Burman design as well as response surface methodology (RSM). A laccase mediator system was designed for the delignification of saw dust which was molecularly characterized by high-performance liquid chromatography (HPLC), Fourier transformation infrared spectroscopy (FTIR) and scanning electron microscopy (SEM). Results The present study reveals wheat bran is a potential substrate for the production of laccase (63 U/g and 9.6 mg/ g protein) under solid-state fermentation by Trichoderma harzianum strain HZN10. Statistical optimization by RSM using central composite design (CCD) revealed that wheat bran contributed maximally to the overall laccase production followed by yeast extract. Laccase production under optimized conditions yielded 510 U/g with 8.09-fold increase. HPLC peaks representing 4-hydroxy-3-methoxybenzoic (vanillic) acid and 4-hydroxy-3,5-dimethoxybenzoic (syringic) acid showed drastic reduction in laccasetreated saw dust sample indicating the elimination of toxic inhibitors, thereby signifying the detoxification of sample.The laccase-treated saw dust showed 1.6-fold increase in reducing sugars after enzymatic (cellulase) hydrolysis. The FTIR analysis revealed the structural alterations occurring during the delignification process. SEM of biologically treated saw dust revealed the morphological alterations during the delignification process targeting the fiber cell walls rich in lignin. Conclusion The delignification of saw dust was effective by laccase mediator system and was evidenced by HPLC, FTIR and SEM analysis. Hence, laccase can be a powerful tool in biomass to biofuel conversions.

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Section: Sem Analysismentioning

confidence: 97%

Optimization of laccase production and its application in delignification of biomass

Bagewadi

2017

Int J Recycl Org Waste Agricult

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“…Keunggulan tersebut antara lain dapat mengolah berbagai jenis kayu baik sejenis maupun campuran, selain itu waktu pemasakan yang relatif pendek, menghasilkan rendemen dan kualitas pulp yang tinggi (Singh, Sulaiman, Hashim, Rupani, & Peng, 2010). Namun disamping keunggulan tersebut, proses pembuatan pulp ini juga memiliki kelemahan, yaitu menghasilkan gas berbau berupa hidrogen sulfida (H2S) dan metil merkaptan (CH3SH) serta warna pulp yang gelap (Liew et al, 2011). Hasil yang demikian ini menyebabkan tingginya kebutuhan bahan pemutih dan bahan organik terklorinasi dari limbah proses pemutihan pulp yang tidak dapat didaur ulang.…”

Section: Pendahuluanunclassified

Physical Damages of Wood Fiber in Acacia Mangium due to Biopulping Treatment

Yahya¹,

Mucharromah²,

Devi³

et al. 2016

Molekul

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ABSTRAKBiopulping adalah pemberian jamur pada serpih untuk mengurangi komponen kimia material yang tidak diinginkan dalam pembuatan pulp. Penelitian pendahuluan menunjukkan bahwa biopulping terhadap kayu mangium berupa pemberian jamur Phanerochaeta chrysosporium dapat mendegradasi lignin dan meningkatkan persentase kadar holoselulosa dan α-selulosa. Selain komponen kimia, dimensi serat juga sangat menentukan kualitas kertas yang dihasilkan. Pertanyaannya adalah bagaimana keutuhan dan dimensi serat dari kayu yang telah diberi jamur tersebut. Penelitian ini bertujuan mengetahui dampak pemberian jamur P. chrysosporium terhadap keutuhan dan dimensi serat. Kegiatan riset dimulai dengan memperbanyak biakan murni P. chrysosporium selama 14 hari di dalam media pertumbuhan, kemudian diinokulasikan ke serpih kayu pada konsentrasi 5% dengan waktu inkubasi 0, 15 dan 30 hari. Serpih hasil inokulasi dibuat stik berukuran 1 mm x 1 mm x 20 mm, dan kemudian dimaserasi dengan franklin solution pada suhu 60 o C selama 48 jam. Pengamatan kerusakan dilakukan terhadap 40 serat untuk setiap perlakuan menggunakan mikroskop pada pembesaran 400x. Dilakukan juga pengukuran dimensi serat hasil inokulasi. Diperoleh hasil bahwa serpih batang mangium yang telah diinokulasi menunjukkan persentase kerusakan serat 0%. Serat-serat hasil inokulasi memiliki panjang yang terkategorikan sedang dan dinding yang tergolong tipis. Kata kunci : biopulping, dimensi serat, keutuhan serat, mangium ABSTRACTBiopulping is fungal pretreatment of wood chips to reduce unused chemical composition of material in pulping. Preliminary study showed that pretreatment of Phanerochaeta chrysosporium to Acacia mangium Willd can reduce lignin and improve holocellulose and cellulose content of the material. Fiber dimension recognized as other important factor for paper properties. The question is how the integrity and dimensions of the wood fiber that has been pretreated with the fungus. The objectives of present study were to know effect of pretreatment of P. chrysosporium to the integrity and dimensions of the fiber. The P. chrysosporium was cultured for 14 days in growth medium, and inoculated to wood chips 5% (w/v) and incubated for 0, 15 and 30 days. The inoculated wood chips were chipped into 1 mm x 1 mm x 20 mm and macerated using franklin solution at 60 o C for 48 hours. Forty fibers from each incubated time were analized their physical damages using a light microscope at a 400 magnification. The inoculated fibers were measured theirs dimensions. The physical damage percentage of fibers pretreated using P. chrysosporium was 0%. Length and wall thickness of the pretreated fibers were can be categorized as middle class and thin fibers, respectively.

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“…WSLig-RF broth was vacuum filtered to separate the mycelia, and the remnant was washed using 500 mL distilled water, followed by drying at 70°C for 24 h. Then, the extent of WSLig-RF depolymerisation was calculated by determining the lost weight as a percentage of the initial weight (Liew et al, 2011).…”

Section: Determination Of the Extent Of Wslig-rf Depolymerisation By mentioning

confidence: 99%

Production of short-chain fatty acids from the biodegradation of wheat straw lignin by Aspergillus fumigatus

Baltierra-Trejo

Sanchez–Yáñez

Buenrostro-Delgado

et al. 2015

Bioresource Technology

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Lignin biodegradation and ligninolytic enzyme studies during biopulping of Acacia mangium wood chips by tropical white rot fungi

Cited by 50 publications

References 23 publications

Optimization of laccase production and its application in delignification of biomass

Optimization of laccase production and its application in delignification of biomass

Physical Damages of Wood Fiber in Acacia Mangium due to Biopulping Treatment

Production of short-chain fatty acids from the biodegradation of wheat straw lignin by Aspergillus fumigatus

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