Lignin content and peroxidase activity in soybean seed coat susceptible and resistant to mechanical damage

Abstract

Key words: ge netic vari abil ity, lignothioglycolic acid, pro tein-free lignin, soy bean cultivars Ab stractMe chan i cal dam age is one of the causes of great loss in the qual ity of soy bean seeds dur ing har vest and pro cess ing. Consid er able in ter est ex ists in the lignin since its de po si tion in the seed coat tis sue pro vides me chan i cal re sis tance and pro tects the cell against mi cro or gan isms. In ad di tion, peroxidases might be in volved in the ox i da tion of cinnamyl al co hols prior to their poly mer iza tion dur ing lignin for ma tion. Thus, the aim of the pres ent work was to an a lyze the lignin con tents and peroxidases ac tiv i ties of six Bra zil ian soy bean cultivars (Savana, Paranagoiana, FT-10, Santa Rosa, Doko and Paraná) and their re la tion ships with the me chan i cal dam age. Re sults showed that the lignin con tent and peroxidase ac tiv ity in the seed coat sig nif i cantly dif fered among the soy bean cultivars. Cultivars Doko and Paraná had the high est con tents of lignin and peroxidases ac tiv i ties while the other cultivars had low est lignin con tents and en zyme ac tiv i ties. Lignin con tent and peroxidase ac tiv ity may be rea son able in di ca tors of re sis tance to me chan i cal dam age in soy bean seeds.

Physical and chemical properties of soybean seeds determine their susceptibility to mechanical damage

“…Seed elasticity plays an important role in the first stage of crushing, and its viscosity -in the subsequent stages (Dobrzański, Stępniewski, 2013). The occurrence of damage also depends on the size and shape of seeds as well as seed coat thickness and chemical composition (Capeleti et al, 2005;Rybiński et al, 2013). Karaj and Müller (2010) and Dobrzański and Stępniewski (2013) demonstrated that seed resistance to mechanical damage significantly decreases together with an increase in seed weight.…”

Glyphosate affects lignin content and amino acid production in glyphosate-resistant soybean

“…As the main structural component of secondarily thickened plant cell walls, lignin contributes to the compression strength of stems to provide mechanical support (Boudet 2000;Boudet et al 2003) and to the efficient transport of water and solutes over long distances within the vascular system (Bernards et al 2000;Donaldson 2001). Lignin in seed coat tissues provides resistance against mechanical damage and protects the cell from microbial attack (Capeleti et al 2005).…”

Isolation of a cinnamoyl CoA reductase gene involved in formation of stone cells in pear (Pyrus pyrifolia)

“…Lignin derives from the dehydrogenative polymerization of three different hydroxycinnamyl alcohols, p-coumaryl alcohol, conferyl alcohol and sinapyl alcohol (Ma 2007), which was believed to play an important role in cell wall thickening and stress response (Kuc 1997;Solecka 1997;Kaczkowski 2003;Capeleti et al 2005). So far, it is generally accepted that cinnamoyl CoA reductase (CCR, EC 1.2.1.44) catalyzes the first specific step reaction in lignin monomers synthesis, converting cinnamoyl CoA esters to their corresponding cinnamaldehydes.…”