Lignin-degrading enzyme production was enhanced by the novel transcription factor Ptf6 in synergistic microbial co-culture

Zhang, Qi; Wang, Qiong; Chen, Haixiu; Chen, Lei; Wang, Feng; Gu, Zhenghua; Shi, Guiyang; Liu, Liming; Ding, Zhongyang

doi:10.1016/j.micres.2023.127575

Microbiological Research

2024

DOI: 10.1016/j.micres.2023.127575

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Lignin-degrading enzyme production was enhanced by the novel transcription factor Ptf6 in synergistic microbial co-culture

Qi Zhang,

Qiong Wang,

Haixiu Chen

et al.

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2024

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Cited by 2 publications

References 46 publications

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Enzymatic Degrading Chlorophenol Wastewater by Mixed Strains of Immobilized White Rot Fungi†

Liu,

Lou,

Shao

et al. 2024

Preprint

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To address chlorophenol wastewater pollution, immobilized mixed white rot fungi (WRF) strain microsphere was designed as a solid degradation agent, usinglignin peroxidase(LiP),manganese peroxidase(MnP), andlaccase(Lac) to degradating the wastewater. Considering the diverse physical and chemical properties of the fungal sphere, the immobilization agent formula is optimized and comprehensive environmental factor design response surface analysis are implemented to determine the delivery conditions. Consequently, the 2,4-DCP treatment rate and extracellular enzyme activity for a 1:1 encapsulation ofT. versicolorandP. sajor-cajusignificantly outperform those of individual strains. Using polyvinyl alcohol (PVA), sodium alginate (SA), and biochar as carriers, with sodium dihydrogen phosphate solution as crosslinker and SiO2/zeolite as additives, immobilizing mixed bacteria yielded a high-quality solid agent. This achieved a 99.33% 2,4-DCP degradation rate over 96 hours, with optimal dosage, pH, and initial 2,4-DCP concentration at 11.5 g/L, 5.5, and 40 mg/L. The degradation of 2,4-DCP by WRF selectively removes adjacent chlorine atoms to produce 4-CP, enhancing the dechlorination efficiency.Graphical Abstract

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Enzymatic Degrading Chlorophenol Wastewater by Mixed Strains of Immobilized White Rot Fungi†

Liu,

Lou,

Shao

et al. 2024

Preprint

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Semi-automated monitoring of longitudinal microbial metabolic dynamics: A study case for lignin degradation

Guimarães Ferreira,

Arini,

da Rosa-Garzon

et al. 2024

Preprint

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This study introduces a low-cost, open-source, semi-automated bioreactor system, developed using Arduino and Raspberry Pi, for longitudinal microbial culture studies. The system protocol includes detailed instructions for custom-designed components, specifications for easily purchasable parts, and open-source code for a web-based control interface. It was validated for sterility and tested in a case study involving the cultivation of Phanerochaete chrysosporium and Trichoderma reesei to assess their metabolic dynamics, both in isolation and co-culture, for lignin degradation. Using mass spectrometry coupled with gas chromatography, several lignin degradation intermediates were annotated, including 4-hydroxybenzoic acid, vanillic acid, and ferulic acid, along with their temporal variation profiles over 25 days. The results highlighted potential synergy between the two fungal species in lignin breakdown, while also demonstrating the bioreactor's flexibility and suitability for diverse biotechnological applications, particularly in the field of bio-degradation and waste valorization.

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Lignin-degrading enzyme production was enhanced by the novel transcription factor Ptf6 in synergistic microbial co-culture

Cited by 2 publications

References 46 publications

Enzymatic Degrading Chlorophenol Wastewater by Mixed Strains of Immobilized White Rot Fungi†

Enzymatic Degrading Chlorophenol Wastewater by Mixed Strains of Immobilized White Rot Fungi†

Semi-automated monitoring of longitudinal microbial metabolic dynamics: A study case for lignin degradation

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