Lignin-Degrading Enzymes of the Commercial Button Mushroom, Agaricus bisporus

Abstract

Agaricus bisporus, grown under standard composting conditions, was evaluated for its ability to produce lignin-degrading peroxidases, which have been shown to have an integral role in lignin degradation by wood-rotting fungi. The activity of manganese peroxidase was monitored throughout the production cycle of the fungus, from the time of colonization of the compost through the development of fruit bodies. Characterization of the enzyme was done with a crude compost extract. Manganese peroxidase was found to have a pl of 3.5 and a pH optimum of 5.4 to 5.5, with maximal activity during the initial stages of fruiting (pin stage). The activity declined considerably with fruit body maturation (first break). This apparent developmentally regulated pattern parallels that observed for laccase activity and for degradation of radiolabeled lignin and synthetic lignins by A. bisporus. Lignin peroxidase activity was not detected in the compost extracts. The correlation between the activities of manganese peroxidase and laccase and the degradation of lignin in A. bisporus suggests significant roles for these two enzymes in lignin degradation by this fungus.

Veratryl alcohol stimulates fruiting body formation in the oyster mushroom, Pleurotus ostreatus

“…The conclusions drawn from this observation were that laccases and, veratryl alcohol too, played a role in the physiological development of the basidiocarp. A role for laccases during fruit body formation has also been observed in A. bisporus [18].…”

Resource allocation ability of wild isolates of Agaricus bisporus on conventional mushroom compost

“…during the vegetative phase, appeared to be dependent on a moderate production of enzyme activities degrading lignocellulose and microbial products. The extracellular enzymes assayed in the present work are all known to be produced by commercial strains of A. bisporus in compost or in liquid media [25,26], but enzyme production in compost by newly isolated wild strains has not been reported before. A contribution to the definition of the adaptation to mushroom compost is presented here.…”

Production of Ligninolytic Enzymes from Penicillium Sp. and Its Efficiency to Decolourise Textile Dyes

“…The enzyme activity was expressed in IU where one unit of Lignin Peroxidase corresponded to the amount of enzyme that oxidized one micromole of veratryl alcohol per min [33]. Manganese Peroxidase activity was quantified using a reaction mixture containing crude enzyme 1mM guaiacol, 10mM citrate phosphate buffer (pH 5.5), 1mM MnSO4, and 50 µM H 2 O 2 in a total volume of 1 ml [34]. The reaction was monitored by measuring the increase in absorbance of the reaction product at 465 nm.…”