Lignin properties and cell wall response to deconstruction by alkaline pretreatment and enzymatic hydrolysis in brown midrib sorghums

Singh, Sandip K.; K., Saulnier, Brian; Hodge, David B.

doi:10.1016/j.indcrop.2022.114566

Cited by 7 publications

(1 citation statement)

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“…Alkaline hydrolysis was used to determine the amount of p -coumarate contained within pretreated biomass and extracted lignin using the same approach as in our prior work . Gel permeation chromatography (GPC) was used to determine the weight- and number-average molar masses of extracted lignins according to previously published methods using a calibration curve constructed from Readycal polystyrene standards (Fluka Analytical) in the 250–70,000 Da range. Elemental analysis of select recovered lignins was performed by inductively coupled plasma-optical emission spectrometry (ICP-OES) with samples prepared according to AOAC 985.01.…”

Section: Methodsmentioning

confidence: 99%

Effect of Dilute Acid Pretreatment and Lignin Extraction Conditions on Lignin Properties and Suitability as a Phenol Replacement in Phenol-Formaldehyde Wood Adhesives

Siahkamari

Singh

et al. 2022

J. Agric. Food Chem.

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Corn stover was subjected to dilute sulfuric acid pretreatment to assess the impact of pretreatment conditions on lignin extractability, properties, and utility as a phenol replacement in wood phenol-formaldehyde (PF) adhesives. It was identified that both formic acid and NaOH could extract and recover 60−70% of the lignin remaining after pretreatment and enzymatic hydrolysis under the mildest pretreatment conditions while simultaneously achieving reasonable enzymatic hydrolysis yields (>60%). The availability of reaction sites for the incorporation of lignins into the PF polymer matrix (i.e., unsubstituted phenolic hydroxyl groups) was shown to be strongly impacted by the pretreatment time and the recovery. Finally, a lignin-based wood adhesive was formulated by replacing 100% of the phenol with formic-acid-extracted lignin, which exhibited a dry shear strength exceeding a conventional PF adhesive. These findings suggest that both pretreatment and lignin extraction conditions can be tailored to yield lignins with properties targeted for this co-product application.

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Section: Methodsmentioning

confidence: 99%