Lignin-protein complex catalyzed by peroxidase

Whitmore, F. W.

doi:10.1016/0304-4211(78)90102-5

Cited by 81 publications

(25 citation statements)

References 5 publications

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“…Benhamou et al (1991) consider that the major epitopes recognized by the HRGP 2b antiserum are on the polypeptide moiety of the molecule. The accumulation of HRGPs within deposits and cell walls prior to the detection of phenolic compounds at these sites is consistent with their proposed role in providing nucleation sites for lignin-like polymer deposition (Whitmore, 1978). HRGPs may also interact ionically with other wall components.…”

Section: Discussionsupporting

confidence: 56%

Hrp Mutant of Pseudomonas syringae pv phaseolicola Induces Cell Wall Alterations but Not Membrane Damage Leading to the Hypersensitive Reaction in Lettuce

1995

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Both wild-type (521 -WT) and hrpD-621-533) strains of Pseudomonas syringae pv phaseolicola induced the formation of large paramural papillae in lettuce (Lacfuca sativa) mesophyll cells adjacent to bacterial colonies. Localized alterations to the plant cell wall included deposition of hydroxyproline-rich glycoproteins, phenolics, and callose, and were associated with proliferation of the endoplasmic reticulum and multivesicular bodies. Tissue collapse during the hypersensitive reaction caused by S21 -WT was associated with electrolyte leakage and rapid accumulation of the phytoalexin lettucenin A, both of which followed membrane damage indicated by the failure of mesophyll cells to plasmolyze. A few cells lost the ability to plasmolyze after inoculation with S21-533, and low levels of lettucenin A were recorded, but neither leakage of electrolytes nor tissue collapse were detected. Dysfunction of the plasma membrane in cells adjacent to colonies of S21-WT led to extensive vacuolation of the cytoplasm, organelle disruption, and cytoplasmic collapse-changes unlike those occurring in cells undergoing apoptosis. Strain S21-533 remained viable within symptomless tissue, whereas cells of S21-WT were killed as a consequence of the hypersensitive reaction. Our observations emphasize the subtle coordination of the plant's response occurring at the subcellular Ievel.

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Section: Discussionsupporting

confidence: 56%

Hrp Mutant of Pseudomonas syringae pv phaseolicola Induces Cell Wall Alterations but Not Membrane Damage Leading to the Hypersensitive Reaction in Lettuce

1995

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“…HRGPs are the major structural proteins of plant cell walls (26)(27)(28). The accumulation of HRGPs occurs in response to wounding (29,30) or infection (17)(18)(19)(31)(32)(33) and is correlated with the expression of increased disease resistance (31)(32)(33)(34)(35)(36). Consistent with these results, rapid accumulation of HRGP mRNAs occurs in response to fungal infection (33).…”

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confidence: 69%

Plant defense genes are regulated by ethylene

Ecker

Davis

1987

Proc. Natl. Acad. Sci. U.S.A.

326

174

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One of the earliest detectable events during plant-pathogen interaction is a rapid increase in ethylene biosynthesis. This gaseous plant stress hormone may be a signal for plants to activate defense mechanisms against invading pathogens such as bacteria, fungi, and viruses. The effect of ethylene on four plant genes involved in three separate plant defense response pathways was examined; these included (i and ii) genes that encode L-phenylalanine ammonia-lyase (EC 4.3.1.5) and 4-coumarate:CoA ligase [4-coumarate:CoA ligase (AMP-forming), EC 6.2.1.12], enzymes of the phenylpropanoid pathway, (iii) the gene encoding chalcone synthase, an enzyme of the flavonoid glycoside pathway, and (iv) the genes encoding hydroxyproline-rich glycoprotein, a major protein component(s) of plant cell walls. Blot hybridization analysis of mRNA from ethylene-treated carrot roots reveals marked increases in the levels of phenylalanine ammonia-lyase mRNA, 4-coumarate CoA ligase mRNA, chalcone synthase mRNA, and certain hydroxyproline-rich glycoprotein transcripts. The effect of ethylene on hydroxyproline-rich glycoprotein mRNA accumulation was different from that of wounding. Ethylene induces two hydroxyproline-rich glycoprotein mRNAs (1.8 and 4.0 kilobases), whereas wounding of carrot root leads to accumulation of an additional hydroxyproline-rich mRNA (1.5 kilobases). These results indicate that at least two distinct signals, ethylene and a wound signal, can affect the expression of plant defense-response genes.

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“…That peroxidase may function as a cross-linking agent is supported by evidence that it catalyzes IDT and diferulate crosslinking (7,14), and binding between lignin and protein (30) and that high levels of the enzyme are not found in the regions of highest growth (17). The fact that we found shoot inversion and Ethrel treatments both to enhance peroxidase activity and to inhibit elongation in the Pharbitis stem are consistent with this hypothesis.…”

Section: Discussionsupporting

confidence: 79%

Shoot Inversion Inhibition of Stem Elongation in Pharbitis nil

Prasad

Cline²

1987

Plant Physiol.

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Inversion of the upper shoot of Pharbitis nil results in the inhibition of elongation in the inverted stem. The objective of the present study was to determine how shoot inversion-induced gravity stress inhibited elongation and to elucidate the possible role of ethylene-induced glycoprotein and lignin in this process. Determinations of hydroxyproline, peroxidase, phenylalanine ammonia-lyase (PAL), phenol, and lignin content/activity were carried out by appropriate spectrophotometric methods. It was found that inversion and Ethrel treatments of upright shoots caused significant increases in hydroxyproline content, peroxidase, and PAL activity in 12 hours and in phenol and lignin contents in 24 hours. All of these increases except for that of cytoplasmic peroxidase activity were partially reversed by AgNO3, the ethylene action inhibitor. It is concluded that possible cross-linking associated with the accumulation of ethyleneinduced hydroxyproline-rich glycoprotein and lignin may be responsible for the later stages of cessation of elongation in the inverted Pharbitis shoot.Shoot inversion-induced release of apical dominance in Pharbitis nil is preceeded by inhibition of elongation of the inverted stem (6,21). This inhibition is apparently due largely to gravity stress-induced ethylene production in the terminal 10 cm of the inverted shoot (20; Fig. 1). There is evidence to suggest that ethylene inhibits elongation by altering cell wall mechanical properties via modification of the orientation of cellular microfibrils, cell wall pectic fraction, or of cell-wall cross-linking ( 12).There have been a number of studies relating accumulation of Hp2 in the cell wall to cessation of elongation (5,24,25 (NPK,20:20:20)

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Lignin-protein complex catalyzed by peroxidase

Cited by 81 publications

References 5 publications

Hrp Mutant of Pseudomonas syringae pv phaseolicola Induces Cell Wall Alterations but Not Membrane Damage Leading to the Hypersensitive Reaction in Lettuce

Hrp Mutant of Pseudomonas syringae pv phaseolicola Induces Cell Wall Alterations but Not Membrane Damage Leading to the Hypersensitive Reaction in Lettuce

Plant defense genes are regulated by ethylene

Shoot Inversion Inhibition of Stem Elongation in Pharbitis nil

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