Lignin reduction in sugarcane by performing CRISPR/Cas9 site-direct mutation of SoLIM transcription factor

Laksana, Chanakan; Sophiphun, Onsulang; Chanprame, Sontichai

doi:10.1016/j.plantsci.2024.111987

Cited by 15 publications

(4 citation statements)

References 81 publications

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“…Considering this fact, and the complex and polyploid nature of the sugarcane genome, precise mutation using CRISPR/Cas9 technology could serve as a valuable tool for reducing lignin content. The utilization of a multigene expression/suppression strategy resulted in the hyper-accumulation of triacylglycerol (TAG) and total fa y acids (FA) in the leaves, as two-fold higher than observed in unmodified energy cane [155,156]. The strongest link between TAG accumulation, ZmDGAT1 expression, and SDP1 repression was also observed.…”

Section: Genome Editing For Sugarcane Improvementmentioning

confidence: 98%

Genetic Engineering for Enhancing Sugarcane Tolerance to Biotic and Abiotic Stresses

Kumar,

Wang,

et al. 2024

Plants

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Sugarcane, a vital cash crop, contributes significantly to the world’s sugar supply and raw materials for biofuel production, playing a significant role in the global sugar industry. However, sustainable productivity is severely hampered by biotic and abiotic stressors. Genetic engineering has been used to transfer useful genes into sugarcane plants to improve desirable traits and has emerged as a basic and applied research method to maintain growth and productivity under different adverse environmental conditions. However, the use of transgenic approaches remains contentious and requires rigorous experimental methods to address biosafety challenges. Clustered regularly interspaced short palindromic repeat (CRISPR) mediated genome editing technology is growing rapidly and may revolutionize sugarcane production. This review aims to explore innovative genetic engineering techniques and their successful application in developing sugarcane cultivars with enhanced resistance to biotic and abiotic stresses to produce superior sugarcane cultivars.

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Section: Genome Editing For Sugarcane Improvementmentioning

confidence: 98%

Genetic Engineering for Enhancing Sugarcane Tolerance to Biotic and Abiotic Stresses

Kumar,

Wang,

et al. 2024

Plants

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“…Recently, genome editing especially CRISPR is emerging as a revolutionary technology in life sciences, which can realize targeted editing and modification to improve crop varieties according to breeding needs (Liu et al, 2022;Nerkar et al, 2022;Adeel and Jones, 2024). With the goal of high yield, high sugar and low fiber, a technical route for genome editing has now been established in sugarcane (Jung and Altpeter, 2016;Rahman et al, 2019;Laksana et al, 2024). The rapid development of high-throughput genomics technologies has brought life sciences into the era of big data, and omics-based interdisciplinarity is accelerating precision crop breeding (Bohra et al, 2020;Shen et al, 2022).…”

Section: Breeding 20mentioning

confidence: 99%

Sugarcane breeding: a fantastic past and promising future driven by technology and methods

Lu,

Liu,

et al. 2024

Front. Plant Sci.

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Sugarcane is the most important sugar and energy crop in the world. During sugarcane breeding, technology is the requirement and methods are the means. As we know, seed is the cornerstone of the development of the sugarcane industry. Over the past century, with the advancement of technology and the expansion of methods, sugarcane breeding has continued to improve, and sugarcane production has realized a leaping growth, providing a large amount of essential sugar and clean energy for the long-term mankind development, especially in the face of the future threats of world population explosion, reduction of available arable land, and various biotic and abiotic stresses. Moreover, due to narrow genetic foundation, serious varietal degradation, lack of breakthrough varieties, as well as long breeding cycle and low probability of gene polymerization, it is particularly important to realize the leapfrog development of sugarcane breeding by seizing the opportunity for the emerging Breeding 4.0, and making full use of modern biotechnology including but not limited to whole genome selection, transgene, gene editing, and synthetic biology, combined with information technology such as remote sensing and deep learning. In view of this, we focus on sugarcane breeding from the perspective of technology and methods, reviewing the main history, pointing out the current status and challenges, and providing a reasonable outlook on the prospects of smart breeding.

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“…Genome editing approaches, such as CRISPR/Cas9, have revolutionized the field of plant biotechnology through genome engineering, facilitating important trait-driven improvement in major crop species ( Chen et al., 2020 ; Wolabu et al., 2020a , 2020b ; Bottero et al., 2021 ; Zheng et al., 2021 ; Bao et al., 2022 ; Kumar et al., 2022 ; Nerkar et al., 2022 ; Singer et al., 2022 ; Wolabu et al., 2023 ). CIRSPR/Cas9 has been employed to manipulate lignin content in various plant species to improve the efficiency of lignocellulose processing and enhance forage quality, digestibility, and saccharification efficiency ( Park et al., 2017 ; Jang et al., 2021 ; Afifi et al., 2022 ; Roy et al., 2023 ; Wolabu et al., 2023 ; Laksana et al., 2024 ). A recent study that altered flowering time via multiplex-CRISPR/Cas9 edition of a florigen in alfalfa generated promising lines with improved biomass yield and quality ( Wolabu et al., 2023 ).…”

Section: Introductionmentioning

confidence: 99%

“…Edited lines exhibited varying degrees of loss-of-function mutations in the target genes with biallelic loss-of-function editing of all target genes ( Sulis et al., 2023 ). Sugarcane lignin content reduction (up to 51% with high S/G ratio) was achieved using the CRISPR/Cas9 genome editing system by targeting SoLIM transcription factors that are involved in the lignin biosynthesis pathway ( Laksana et al., 2024 ). In this regard, modern genome editing approaches offer great opportunities to reduce lignin content and/or to alter composition to improve product quality without compromising plant growth and resilience.…”

Section: Introductionmentioning

confidence: 99%

Mutating alfalfa COUMARATE 3-HYDROXYLASE using multiplex CRISPR/Cas9 leads to reduced lignin deposition and improved forage quality

Wolabu,

Mahmood,

Chen

et al. 2024

Front. Plant Sci.

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Alfalfa (Medicago sativa L.) forage quality is adversely affected by lignin deposition in cell walls at advanced maturity stages. Reducing lignin content through RNA interference or antisense approaches has been shown to improve alfalfa forage quality and digestibility. We employed a multiplex CRISPR/Cas9-mediated gene-editing system to reduce lignin content and alter lignin composition in alfalfa by targeting the COUMARATE 3-HYDROXYLASE (MsC3H) gene, which encodes a key enzyme in lignin biosynthesis. Four guide RNAs (gRNAs) targeting the first exon of MsC3H were designed and clustered into a tRNA-gRNA polycistronic system and introduced into tetraploid alfalfa via Agrobacterium-mediated transformation. Out of 130 transgenic lines, at least 73 lines were confirmed to contain gene-editing events in one or more alleles of MsC3H. Fifty-five lines were selected for lignin content/composition analysis. Amongst these lines, three independent tetra-allelic homozygous lines (Msc3h-013, Msc3h-121, and Msc3h-158) with different mutation events in MsC3H were characterized in detail. Homozygous mutation of MsC3H in these three lines significantly reduced the lignin content and altered lignin composition in stems. Moreover, these lines had significantly lower levels of acid detergent fiber and neutral detergent fiber as well as higher levels of total digestible nutrients, relative feed values, and in vitro true dry matter digestibility. Taken together, these results showed that CRISPR/Cas9-mediated editing of MsC3H successfully reduced shoot lignin content, improved digestibility, and nutritional values without sacrificing plant growth and biomass yield. These lines could be used in alfalfa breeding programs to generate elite transgene-free alfalfa cultivars with reduced lignin and improved forage quality.

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Lignin reduction in sugarcane by performing CRISPR/Cas9 site-direct mutation of SoLIM transcription factor

Cited by 15 publications

References 81 publications

Genetic Engineering for Enhancing Sugarcane Tolerance to Biotic and Abiotic Stresses

Genetic Engineering for Enhancing Sugarcane Tolerance to Biotic and Abiotic Stresses

Sugarcane breeding: a fantastic past and promising future driven by technology and methods

Mutating alfalfa COUMARATE 3-HYDROXYLASE using multiplex CRISPR/Cas9 leads to reduced lignin deposition and improved forage quality

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