Lignin Synthesis, Affected by Sucrose in Lotus (Nelumbo nucifera) Seedlings, Was Involved in Regulation of Root Formation in the Arabidopsis thanliana

Cheng, Long; Chen, Zhao; Minrong, Zhao; Han, Yuan; Li, Shuyan

doi:10.3390/ijms23042250

Cited by 8 publications

(9 citation statements)

References 63 publications

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“…However, whether ethylene signal being involved in drought and salt adaptation is needed further analysis. In previous study, it is founnd that lignin synthesis has a great in uence on ARs development through sucrose pathway [22,23]). In this study, we found that overexpression of NnWOX1-1, NnWOX4-3 and NnWOX5-1 did not NnWOX1-1, NnWOX4-3 and NnWOX5-1 affecting root development of Arabidopsis was not resulted from synthesis of IAA, ABA and lignin.…”

Section: Discussionmentioning

confidence: 99%

“…Light and photosynthetic products (sucrose) is also found to have a great in uence on the formation of ARs [21,22]. It is reported that lignin is involved in lotus ARs formation, which is also regulated by sucrose, suggesting that the development of ARs also has a complex regulatory network in lotus [23]. In addtition, ABA and cytokinin are also found to be involved in ARs formation [11,24].…”

Section: Introductionmentioning

confidence: 95%

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The lotus NnWOX1-1、NnWOX4-3 and NnWOX5-1 promoted root formation and enhanced stresses tolerance in transgenic Arabidopsis thaliana

quan,

Shiting,

Chen

et al. 2023

Preprint

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Background: Adventitious root (AR) is an important organ in water and nutrition uptake in lotus due to degeneration of principle root. Wox is involved in regulating plant development and growth by affecting multiple genes expression in plant kingdom. In this study, three genes including NnWOX1-1、NnWOX4-3 and NnWOX5-1were isolated, and genes function was assessed in Arabidopsis plants. Results: The full-length of the NnWOX1-1、NnWOX4-3 and NnWOX5-1 were 1038 bp, 645 bp and 558 bp in nucleotide, and encoded 362, 345 and 185 amino acid residues respectively. Phylogenetic analysis showed that Nnwox1-1 and Nnwox4-3 was classified into one group, and Nnwox5-1 and Mnwox5 showed high genetic relationship. These three genes were all induced by sucrose and IAA, and had organ-speciﬁc expression characteristic. Aside from the improvement of root growth and salt tolerance, NnWOX1-1 and NnWOX4-3 could also promote stem development in transgenic Arabidopsis. A total of 751, 594 and 541genes including nineteen, nineteen and thirteen genes related with ethylene and IAA metabolism and response were enhanced expression in NnWOX1-1、NnWOX4-3 and NnWOX5-1 transgenic plants respectively. Further analysis showed that ethylene product rates was increased, and while IAA, POD and lignin content were no significantly changed. Exogenous application of ethephon promoted ARs formtion and dramaticly increased fresh weight and dry weight of lotus seedlings. Conclusions: NnWOX1-1、NnWOX4-3 and NnWOX5-1 was involved in root formation, stem development and stresses adaptation in transgenic Arabidopsis plants by affecting multiple genes transcriptation. Among which, the change of genes expression in ethylene metabolism and respondings probably exerted criticle role on development in Arabidopsisplant. In addition, ethylene might be an important factor to affect ARs formation in lotus seedlings.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 95%

The lotus NnWOX1-1、NnWOX4-3 and NnWOX5-1 promoted root formation and enhanced stresses tolerance in transgenic Arabidopsis thaliana

quan,

Shiting,

Chen

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

“…Plant root development is negatively correlated with lignin content, and excessive lignin accumulation inhibits the development and elongation of roots in the case of Arabidopsis and carrot. Treatment of plants with exogenous IBA resulted in decreased lignin accumulation and increased root length [ 52 ]. Lignin content not only affects root development, but also affects fruit size.…”

Section: Discussionmentioning

confidence: 99%

CcNAC1 by Transcriptome Analysis Is Involved in Sudan Grass Secondary Cell Wall Formation as a Positive Regulator

Huang

Chen

Liu

et al. 2023

IJMS

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Sudan grass is a high-quality forage of sorghum. The degree of lignification of Sudan grass is the main factor affecting its digestibility in ruminants such as cattle and sheep. Almost all lignocellulose in Sudan grass is stored in the secondary cell wall, but the mechanism and synthesis of the secondary cell wall in Sudan grass is still unclear. In order to study the mechanism of secondary cell wall synthesis in Sudan grass, we used an in vitro induction system of Sudan grass secondary cell wall. Through transcriptome sequencing, it was found that the NAC transcription factor CcNAC1 gene was related to the synthesis of the Sudan grass secondary cell wall. This study further generated CcNAC1 overexpression lines of Arabidopsis to study CcNAC1 gene function in secondary cell wall synthesis. It was shown that the overexpression of the CcNAC1 gene can significantly increase lignin content in Arabidopsis lines. Through subcellular localization analysis, CcNAC1 genes could be expressed in the nucleus of a plant. In addition, we used yeast two-hybrid screening to find 26 proteins interacting with CcNAC1. GO and KEGG analysis showed that CcNAC1 relates to the metabolic pathways and biosynthesis of secondary metabolites. In summary, the synthesis of secondary cell wall of Sudan grass can be regulated by CcNAC1.

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“…8). Indeed, it has been reported that lignin deposition and overaccumulation negatively affects root growth (Cheng et al ., 2022). According to previous studies, auxin participates in the regulation of cell wall modifications, including lignin deposition (Majda & Robert, 2018).…”

Section: Discussionmentioning

confidence: 99%

Disruption of the amino acid transporter CsAAP2 inhibits auxin‐mediated root development in cucumber

Yao

Nie

et al. 2023

New Phytologist

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Amino acid transporters are the principal mediators of organic nitrogen distribution within plants and are essential for plant growth and development. Despite this importance, relatively few amino acid transporter genes have been explored and elucidated in cucumber (Cucumis sativus).Here, a total of 86 amino acid transporter genes were identified in the cucumber genome. We further identified Amino Acid Permease (AAP) subfamily members that exhibited distinct expression patterns in different tissues.We found that the CsAAP2 as a candidate gene encoding a functional amino acid transporter is highly expressed in cucumber root vascular cells. CsAAP2 knockout lines exhibited arrested development of root meristem, which then caused the delayed initiation of lateral root and the inhibition of root elongation. What is more, the shoot growth of aap2 mutants was strongly retarded due to defects in cucumber root development.Moreover, aap2 mutants exhibited higher concentrations of amino acids and lignin in roots. We found that the mutant roots had a stronger ability to acidize medium. Furthermore, in the aap2 mutants, polar auxin transport was disrupted in the root tip, leading to high auxin levels in roots. Interestingly, slightly alkaline media rescued their severely reduced root growth by stimulating auxin pathway.

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Lignin Synthesis, Affected by Sucrose in Lotus (Nelumbo nucifera) Seedlings, Was Involved in Regulation of Root Formation in the Arabidopsis thanliana

Cited by 8 publications

References 63 publications

The lotus NnWOX1-1、NnWOX4-3 and NnWOX5-1 promoted root formation and enhanced stresses tolerance in transgenic Arabidopsis thaliana

The lotus NnWOX1-1、NnWOX4-3 and NnWOX5-1 promoted root formation and enhanced stresses tolerance in transgenic Arabidopsis thaliana

CcNAC1 by Transcriptome Analysis Is Involved in Sudan Grass Secondary Cell Wall Formation as a Positive Regulator

Disruption of the amino acid transporter CsAAP2 inhibits auxin‐mediated root development in cucumber

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