Lignin synthesis mediated by CCoAOMT enzymes is required for the tolerance against excess Cu in Oryza sativa

Su, Nana; Ling, Fei; Xing, Aiming; Zhao, Haihao; Zhu, Yongwei; Wang, Ying; Deng, Xiaopeng; Wang, Chunfei; Xu, Xiaoxia; Hu, Zhubing; Chen, Jin; Shen, Zhenguo; Xia, Yan

doi:10.1016/j.envexpbot.2020.104059

Cited by 50 publications

(17 citation statements)

References 64 publications

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“…Apart from these proteins and metals, various secondary metabolites also act as metal chelators and are involved in the sequestration of HMs to vacuole to provide HM-stress tolerance ( Figure 2 ). Su et al (2020) investigated the role of lignin in HM-stress tolerance and found that two lignin-defective mutants ( ldm1 and ldm2 ) of rice showed dwarf shoots and roots due to higher levels of Cu accumulation in the roots and leaf sheaths tissues, suggesting that the promotion of lignin synthesis in plants may be an adaptive strategy for Cu adaptation [ 171 ]. Interestingly, a higher accumulation of phenolics and amino acids was found to be associated with Co and Cu stress tolerance.…”

Section: Sequestration and Compartmentalization: Plants’ Way To Alleviate The Hm Toxicitymentioning

confidence: 99%

A Comprehensive Review on the Heavy Metal Toxicity and Sequestration in Plants

et al. 2021

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Heavy metal (HM) toxicity has become a global concern in recent years and is imposing a severe threat to the environment and human health. In the case of plants, a higher concentration of HMs, above a threshold, adversely affects cellular metabolism because of the generation of reactive oxygen species (ROS) which target the key biological molecules. Moreover, some of the HMs such as mercury and arsenic, among others, can directly alter the protein/enzyme activities by targeting their –SH group to further impede the cellular metabolism. Particularly, inhibition of photosynthesis has been reported under HM toxicity because HMs trigger the degradation of chlorophyll molecules by enhancing the chlorophyllase activity and by replacing the central Mg ion in the porphyrin ring which affects overall plant growth and yield. Consequently, plants utilize various strategies to mitigate the negative impact of HM toxicity by limiting the uptake of these HMs and their sequestration into the vacuoles with the help of various molecules including proteins such as phytochelatins, metallothionein, compatible solutes, and secondary metabolites. In this comprehensive review, we provided insights towards a wider aspect of HM toxicity, ranging from their negative impact on plant growth to the mechanisms employed by the plants to alleviate the HM toxicity and presented the molecular mechanism of HMs toxicity and sequestration in plants.

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Section: Sequestration and Compartmentalization: Plants’ Way To Alleviate The Hm Toxicitymentioning

confidence: 99%

A Comprehensive Review on the Heavy Metal Toxicity and Sequestration in Plants

et al. 2021

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“…As a barrier for plant defense against both abiotic and biotic stresses, lignin also plays an important role in the resistance of plants to excess heavy metals. It is speculated that the toxicity of Cu could be alleviated by promoting lignin biosynthesis [ 17 ]. Furthermore, excess Cu increased laccase activity in rice, and the expression of Cu-induced OsLAC10 in Arabidopsis enhanced the tolerance of transgenic plants to Cu stress [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

Genome-Wide Identification of Switchgrass Laccases Involved in Lignin Biosynthesis and Heavy-Metal Responses

Zhao

et al. 2022

IJMS

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Plant laccase genes belong to a multigene family, play key roles in lignin polymerization, and participate in the resistance of plants to biotic and abiotic stresses. Switchgrass is an important resource for forage and bioenergy production, yet information about the switchgrass laccase gene family is scarce. Using bioinformatic approaches, a genome-wide analysis of the laccase multigene family in switchgrass was carried out in this study. In total, 49 laccase genes (PvLac1 to PvLac49) were identified; these can be divided into five subclades, and 20 of them were identified as targets of miR397. The tandem and segmental duplication of laccase genes on Chr05 and Chr08 contributed to the expansion of the laccase family. The laccase proteins shared conserved signature sequences but displayed relatively low sequence similarity, indicating the potential functional diversity of switchgrass laccases. Switchgrass laccases exhibited distinct tissue/organ expression patterns, revealing that some laccases might be involved in the lignification process during stem development. All five of the laccase isoforms selected from different subclades responded to heavy metal. The immediate response of lignin-related laccases, as well as the delayed response of low-abundance laccases, to heavy-metal treatment shed light on the multiple roles of laccase isoforms in response to heavy-metal stress.

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“…Previous studies indicated that ligni cation might be an important step of root growth reduction in plants exposed to abiotic stresses but that cell-wall ligni cation in response to extreme environment provided structural rigidity and durability to plant tissues (Cesarino, 2019). Moreover, the increase of lignin production and the up-regulation of related gene expression in plant were associated with heavy metal tolerance (Su et al 2020). Several enzymes, such as phenylalanine ammonia-lyase (PAL), cinnamyl alcohol dehydrogenase (CAD) and 4coumarate:CoA ligase (4CL), play important roles in the synthesis of lignin through the synthesis pathway of phenylpropane secondary substances (Sajad et al 2020).…”

Section: Introductionmentioning

confidence: 99%

WITHDRAWN: Lignin metabolism involved in zinc-alleviating effects on iron-induced phytotoxicity in wheat roots

Zhang

et al. 2022

Preprint

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In order to reveal the mechanisms of Fe-inhibited plant root growth and Zn-alleviating effects on iron (Fe) toxicity, wheat cultivars Xihan 4 seedlings were used to investigate the relationship between root growth and synthetic metabolism of lignin in response to 300 µM Fe stress alone or in combination with 50 or 250 µM zinc (Zn). Fe-alone-exposed roots exhibited significant increase of cell wall lignin content and the stimulation of phenylalanine ammonia-lyase (PAL), 4-coumarate:CoA ligase (4CL), cinnamyl alcohol dehydrogenase (CAD), laccase (LAC), cell wall bound guaiacol peroxidase activity (CW-bound GPX) and conifers alcohol peroxidase (CAPX) activities as well as the up-regulation of TaPAL, Ta4CL, TaCAD and TaLAC expression. In comparison with Fe stress alone, the presence of Zn effectively alleviated the changes of these parameters in Fe-treated roots. And also, the results of lignin staining in the root tissue were consistent with those of lignin detection in wheat seedlings under different treatments. Further study showed the increase of apoplastic hydrogen peroxide (H2O2) content in conjunction with the stimulation of CW-bound nicotinamide adenine dinucleotide (NADH) oxidase, plasma membrane (PM) nicotinamide adenine dinucleotide phosphate (NADPH) oxidase and apoplastic superoxide dismutase (SOD) in Fe-alone-stressed roots compared with untreated ones, but Fe + Zn treatment partially reversed the changes of these parameters in comparison with Fe exposure alone. Furthermore, the application of exogenous H2O2 not only enhanced the amount of cell wall lignin in Zn + Fe-treated roots but also significantly blocked Zn-alleviating effect on Fe-induced inhibition of root growth. In conclusion, the inhibition of root growth was associated with the increased accumulation of cell wall lignin in response to Fe exposure alone, which was due to the stimulation of lignin metabolizing enzymes and the up-regulation of related enzyme gene expression and the increase of apoplastic H2O2 generation in wheat roots under Fe exposure alone. It was also suggested that Zn-alleviating effect on Fe-induced inhibition of root growth might be related to the reduction of lignin content of root cell wall resulting from the decrease of apoplastic H2O2 content when wheat seedlings were exposed to Fe + Zn treatment.

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Lignin synthesis mediated by CCoAOMT enzymes is required for the tolerance against excess Cu in Oryza sativa

Cited by 50 publications

References 64 publications

A Comprehensive Review on the Heavy Metal Toxicity and Sequestration in Plants

A Comprehensive Review on the Heavy Metal Toxicity and Sequestration in Plants

Genome-Wide Identification of Switchgrass Laccases Involved in Lignin Biosynthesis and Heavy-Metal Responses

WITHDRAWN: Lignin metabolism involved in zinc-alleviating effects on iron-induced phytotoxicity in wheat roots

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