Lignocellulolytic enzymes from Aspergillus allahabadii for efficient bioconversion of rice straw into fermentable sugars and biogas

Sharma, Gaurav; Kaur, Baljit; Raheja, Yashika; Agrawal, Dhruv; Basotra, Neha; Falco, Marcos Di; Tsang, Adrian; Chadha, Bhupinder Singh

doi:10.1016/j.biortech.2022.127507

Cited by 15 publications

(3 citation statements)

References 41 publications

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“…Genomics data has potential to provide clues to facilitate the cultivation of S. weigelae . Recent study has shown that CAZymes are crucial for the growth and development of wood-inhabiting fungi and for them to thrive in environments rich in carbohydrates, particularly lignocellulose and cellulose ( Sharma et al., 2022 ; He et al., 2023 ). In the S. weigelae genome, the main CAZymes were GHs, GTs, and AAs, while PLs, CEs, and CMBs were in the minority.…”

Section: Discussionmentioning

confidence: 99%

First genome assembly and annotation of Sanghuangporus weigelae uncovers its medicinal functions, metabolic pathways, and evolution

Jin,

Ma,

Wang

et al. 2024

Front. Cell. Infect. Microbiol.

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Sanghuangporus, also known as “Sanghuang” in China, is a well-known genus of traditional Chinese medicinal macrofungi. To make more effective use of Sanghuangporus resources, we completed the first genome assembly and annotation of a monokaryon strain of S. weigelae in the present study. A 33.96-Mb genome sequence was assembled as 13 contigs, leading to prediction of 9377 protein-coding genes. Phylogenetic and average nucleotide identity analyses indicated that the S. weigelae genome is closely related to those of other Sanghuangporus species in evolutionary tree, which clustered in one clade. Collinearity analysis revealed a high level of collinearity of S. weigelae with S. baumii, S. vaninii, and S. sanghuang. Biosynthesis pathways potentially involved in medicinal properties, including terpenoid and polysaccharide synthesis, were identified in S. weigelae, while polysaccharides were identified as the main medicinal metabolites in S. weigelae, with flavonoids more important in Sanghuangporus than other medicinal mushroom groups. Genes encoding 332 carbohydrate-active enzymes were identified in the S. weigelae genome, including major glycoside hydrolases and glycosyltransferases predicted, revealing the robust lignocellulose degradation capacity of S. weigelae. Further, 130 genes, clustered in seven classes were annotated to encode cytochromes P450 in the S. weigelae genome. Overall, our results reveal the remarkably medicinal capacity of S. weigelae and provide new insights that will inform the study of evolution and medicinal application of S. weigelae. The data are a reference resource for the formulation of scientific and rational ecological protection policies for Sanghuangporus species.

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Section: Discussionmentioning

confidence: 99%

First genome assembly and annotation of Sanghuangporus weigelae uncovers its medicinal functions, metabolic pathways, and evolution

Jin,

Ma,

Wang

et al. 2024

Front. Cell. Infect. Microbiol.

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“…CAZymes are important for the growth and development of wood-rotting mushrooms to thrive in environments rich in carbohydrates, especially lignocellulose and cellulose [ 18 , 56 , 57 , 58 ]. In the O. raphanipes genome, the GHs, GTs, and AAs were the main CAZymes, while PLs, CEs, and CMBs were in the minority.…”

Section: Discussionmentioning

confidence: 99%

Whole Genome Sequence of an Edible Mushroom Oudemansiella raphanipes (Changgengu)

Zhu

Gao²,

Zhang

et al. 2023

JoF

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Oudemansiella raphanipes, considered as a well-known culinary edible mushroom with a high content of natural bioactive substances, is widely cultivated in China with the commercial name Changgengu. However, due to the lack of genomic data, molecular and genetic study on O. raphanipes is rare. To obtain a comprehensive overview of genetic characteristics and enhance the value of O. raphanipes, two mating-compatible monokaryons isolated from the dikaryon were applied for de novo genome sequencing and assembly using Nanopore and /or Illumina sequencing platforms. One of the monokaryons, O. raphanipes CGG-A-s1, was annotated with 21,308 protein-coding genes, of which 56 were predicted to be involved in the biosynthesis of secondary metabolites such as terpene, type I PKS, NRPS, and siderophore. Phylogenetic and comparative analysis of multiple fungi genomes revealed a close evolutionary relationship between O. raphanipes and Mucidula mucid based on single-copy orthologous protein genes. Significant collinearity was detected between O. raphanipes and Flammulina velutipes on the synteny of inter-species genomes. 664 CAZyme genes in CGG-A-s1 were identified with GHs and AAs families significantly elevated when compared with the other 25 sequenced fungi, indicating a strong wood degradation ability. Furthermore, the mating type locus analysis revealed that CGG-A-s1 and CGG-A-s2 were conserved in the gene organization of the mating A locus but various in that of the mating B locus. The genome resource of O. raphanipes will provide new insights into its development of genetic studies and commercial production of high-quality varieties.

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“…This will prove conducive to the performance of a catalyst and to the depolymerization of hemicellulose and cellulose into oligosaccharides or monosaccharides, which can be directly used as valuable chemical and fuel resources such as bioethnol, biogas, biohydrogen, and so forth [15][16][17][18][19]. The current pretreatment and degradation methods include physical methods (ball milling [20], microwaving [21], and ultrasonication [22]), physical and chemical methods (steam explosion [23], supercritical fluid extraction [24], and hydrothermal methods [25]), chemical methods (acids [26], alkaline chemicals [27], ionic liquids [28], organic solvents [29], deep eutectic [30] and metal salts [31]), and biological methods (microorganisms [32] and enzymes [33]). However, some limitations for certain pretreatment methods have been reported: the high cost of reagents in pretreatment with chemical ionic liquids, high investments for the equipment required in ultrasonication, steam explosion, and supercritical pretreatments, consumption of high levels of mechanical energy in the ball milling pretreatment, secondary pollution caused by pretreatments with chemical acids, alkali reagents, organic solvents, and metal salts, or demanding process controls during pretreatments with microorganisms and enzymes [34].…”

Section: Introductionmentioning

confidence: 99%

Comparative Study of Effective Pretreatments on the Structural Disruption and Hydrodepolymerization of Rice Straw

Yang

Liang

et al. 2023

Sustainability

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Rice straw (RS) is the most potentially renewable agricultural waste resource widely distributed in nature. Considering the complex recalcitrant structure and components of RS, three pretreatment methods, including high-temperature hydrothermal, medium-temperature microwave, and low-temperature cryocrushing pretreatment were performed. The components and structure of RS residues were examined and analyzed after the pretreatments. Pretreatment with hydrothermal yielded the lowest rice straw recovery (59.0%); after being pretreated at 180 °C for 10 min, the hemicellulose recovery was only 14.1%, and the removal efficiency of lignin was the largest (32.2%), which was 18.8% higher than that achieved from cryocrushing and microwave pretreatment, respectively. Pretreatment with cryocrushing yielded the highest recovery rates of rice straw (92.9%), hemicellulose and cellulose (88.8% and 90.4%, respectively). Results of scanning electron microscopy, X-ray diffraction, Fourier-transform infrared spectroscopy, and the analysis of specific surface area and apertures demonstrated that all three pretreatments could effectively disrupt the structure of RS and reduce its cellulose crystallinity. The three pretreatments were found to enhance the hydrodepolymerization of RS residues. Furthermore, cryocrushing pretreatment yielded the highest cellulose conversion rate (56.8%), and the yields of glucose, xylose, and arabinose were 29.6%, 56.2%, and 17.8%, respectively. Apart from the use of acids and enzymes, hydrodepolymerization of RS was among the few methods that can effectively degrade cellulose, presenting an ideal solution for the degradation of biomass.

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Lignocellulolytic enzymes from Aspergillus allahabadii for efficient bioconversion of rice straw into fermentable sugars and biogas

Cited by 15 publications

References 41 publications

First genome assembly and annotation of Sanghuangporus weigelae uncovers its medicinal functions, metabolic pathways, and evolution

First genome assembly and annotation of Sanghuangporus weigelae uncovers its medicinal functions, metabolic pathways, and evolution

Whole Genome Sequence of an Edible Mushroom Oudemansiella raphanipes (Changgengu)

Comparative Study of Effective Pretreatments on the Structural Disruption and Hydrodepolymerization of Rice Straw

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