Lignocellulosic Biomass to Fungal Oils: A Radical Bioconversion Toward Establishing a Prospective Resource

Diwan, Batul; Gupta, Pratima

doi:10.1007/978-3-030-14846-1_14

Cited by 4 publications

(5 citation statements)

References 97 publications

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“…Filamentous fungi belonging to the Cunninghamella genus and related genera play an important role in developing sustainable biorefinery processes thanks to their ability to utilize a broad range of renewable feedstock, and waste materials (e.g. corn gluten, corn steep, orange peel and tomato waste hydrolysate) and convert them into SCOs containing significant quantities of GLA (Diwan and Gupta, 2019; Donot et al, 2014; Fakas et al, 2008; Gema et al, 2002).…”

Section: Resultsmentioning

confidence: 99%

“…molasses, peels, fruit marc and seeds and fruit pulp) (Sadh et al, 2018). Among residues, lignocellulosic biomass is the most abundant residue worldwide, and although it can be considered as a promising cost-effective fermentation feedstock (Diwan and Gupta, 2019; Valdés et al, 2020b, 2020a), is treated as waste in many countries (Arevalo-Gallegos et al, 2017). The uncontrollable disposal of agro-industrial residues, especially those produced during industrial processing, to the landfills without any treatment, can cause environmental pollution on the surrounded areas and harmful effects on human and animal health (Ghinea et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

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Bioconversion of pomegranate residues into biofuels and bioactive lipids

Dourou¹,

Economou

Aggeli

et al. 2021

Preprint

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Pomegranate residues (PRs) (i.e. the solid residues remaining after juice extraction), generated currently in abundance in Greece, contain a variety of carbon sources and therefore can be regarded as a potential feedstock for chemical and biotechnological processes rather than as waste materials. In the current project, the polysaccharides contained in PRs were extracted and hydrolyzed in a one-step process without the use of chemical reagents and the resulting broth was used as substrate in biotechnological applications, including ethanol and single cell oil (SCO) production. The yeasts Meyerozyma guilliermondii, Scheffersomyces coipomoensis, Sugiyamaella paludigena and especially Saccharomyces cerevisiae, were able to efficiently convert PR derived reducing sugars into bioethanol. Ethanol production under anaerobic conditions ranged from 3.6 to 12.5 g/L. In addition, the oleaginous yeasts Lipomyces lipofer and Yarrowia lipolytica as well as M. guilliermondii, S. coipomoensis and S. paludigena were tested for their ability to accumulate lipids suitable as feedstock for biodiesel production. Lipids were accumulated at concentrations up to 18% and were rich in palmitic acid (C16:0) and oleic acid (C18:1). Finally, the oleaginous fungus Cunnichamella echinulata was cultivated on PR based solid substrates for γ-linolenic acid (GLA) production. The fermented bio-products (i.e. fermented substrate plus fungal mycelia) contained up to 4.8 mg GLA/g of dry weight. Phenolic removal (up to 30%) was achieved by several of the above mentioned microorganisms, including C. echinulata, L. lipofer, M. guilliermondii, S. paludigena and Y. lipolytica. We conclude that PRs can be used as a raw material for microbial growth, ethanol and SCO production, which is of economic and environmental importance.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Bioconversion of pomegranate residues into biofuels and bioactive lipids

Dourou¹,

Economou

Aggeli

et al. 2021

Preprint

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“…The studied species of filamentous fungi, namely Mucor circinelloides, Umbelopsis vinacea, Absidia glauca, Lichtheimia corymbifera, Cunninghamella blakesleeana, and Amylomyces rouxii, are considered as either model organisms (Mucor circinelloides) or oleaginous filamentous fungi of high industrial potential for production of microbial oils [43]. All species were grown under nitrogen-limitation to facilitate accumulation of lipids in the biomass.…”

Section: Fungal Strainsmentioning

confidence: 99%

Evaluation and optimisation of direct transesterification methods for the assessment of lipid accumulation in oleaginous filamentous fungi

et al. 2021

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Background Oleaginous filamentous fungi can accumulate large amount of cellular lipids and potentially serve as a major source of oleochemicals for food, feed, chemical, pharmaceutical, and transport industries. Transesterification of microbial oils is an essential step in microbial lipid production at both laboratory and industrial scale. Direct transesterification can considerably reduce costs, increase sample throughput and improve lipid yields (in particular fatty acid methyl esters, FAMEs). There is a need for the assessment of the direct transesterification methods on a biomass of filamentous fungi due to their unique properties, specifically resilient cell wall and wide range of lipid content and composition. In this study we have evaluated and optimised three common direct transesterification methods and assessed their suitability for processing of fungal biomass. Results The methods, based on hydrochloric acid (Lewis method), sulphuric acid (Wahlen method), and acetyl chloride (Lepage method), were evaluated on six different strains of Mucoromycota fungi by using different internal standards for gas chromatography measurements. Moreover, Fourier transform infrared (FTIR) spectroscopy was used for the detection of residual lipids in the biomass after the transesterification reaction/extraction, while transesterification efficiency was evaluated by nuclear magnetic resonance spectroscopy. The results show that the majority of lipids, in particular triglycerides, were extracted for all methods, though several methods had substandard transesterification yields. Lewis method, optimised with respect to solvent to co-solvent ratio and reaction time, as well as Lepage method, offer precise estimate of FAME-based lipids in fungal biomass. Conclusions The results show that Lepage and Lewis methods are suitable for lipid analysis of oleaginous filamentous fungi. The significant difference in lipid yields results, obtained by optimised and standard Lewis methods, indicates that some of the previously reported lipid yields for oleaginous filamentous fungi must be corrected upwards. The study demonstrates value of biomass monitoring by FTIR, importance of optimal solvent to co-solvent ratio, as well as careful selection and implementation of internal standards for gas chromatography.

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“…Filamentous fungi belonging to the Cunninghamella genus and related genera play an important role in developing sustainable biorefinery processes thanks to their ability to utilize a broad range of renewable feedstock, and waste materials (e.g. corn gluten, corn steep, orange peel and tomato waste hydrolysate) and convert them into SCOs containing significant quantities of GLA (Diwan and Gupta, 2019;Donot et al, 2014;Fakas et al, 2008;Gema et al, 2002).…”

Section: Microbial Growth On Pr Based Mediamentioning

confidence: 99%

“…molasses, peels, fruit marc and seeds and fruit pulp) (Sadh et al, 2018). Among residues, lignocellulosic biomass is the most abundant residue worldwide, and although it can be considered as a promising cost-effective fermentation feedstock (Diwan and Gupta, 2019;Valdés et al, 2020bValdés et al, , 2020a, is treated as waste in many countries (Arevalo-Gallegos et al, 2017). The uncontrollable disposal of agro-industrial residues, especially those produced during industrial processing, to the landfills without any treatment, can cause environmental pollution on the surrounded areas and harmful effects on human and animal health (Ghinea et al, 2019).…”

mentioning

confidence: 99%