Lignocellulosic Biorefinery for Value-Added Products: The Emerging Bioeconomy

Karp, Susan Grace; Sydney, Eduardo Bittencourt; Woiciechowski, Adenise Lorenci; Letti, Luiz Alberto Júnior; Carvalho, Júlio César de; Torres, Luis Alberto Zevallos; Kumlehn, Gabriel Sprotte; Candeo, Esteffany de Souza; Soccol, Carlos Ricardo

doi:10.1016/b978-0-12-821878-5.00002-7

Cited by 5 publications

(3 citation statements)

References 88 publications

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“…In this context, lignocellulosic biomass continues to attract global interest for different characteristics, such as its abundant availability, renewability, and carbon-neutral nature. Hence, it is becoming an indispensable feedstock for the synthesis of high-value products such as biofuel, platform chemicals, or biomaterials [ 1 , 2 , 3 , 4 , 5 ]. Lignocellulose is found in secondary plant cell walls.…”

Section: Introductionmentioning

confidence: 99%

Characterization of Cellulosic Pulps Isolated from Two Widespread Agricultural Wastes: Cotton and Sunflower Stalks

La Rubia,

Jurado-Contreras,

Navas-Martos

et al. 2024

Polymers

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Globally, huge amounts of cotton and sunflower stalks are generated annually. These wastes are being underutilized since they are mostly burned in the fields. So, in this work, we proposed a three-step method consisting of acid pre-treatment, alkaline hydrolysis, and bleaching for the extraction of cellulose pulps. These pulps were characterized to assess their morpho-structural and thermal properties. The design of experiments and response surface methodology were used for the optimization of the acid pre-treatment in order to achieve maximum removal of non-cellulosic compounds and obtain pulps enriched in cellulose. For cotton stalks, optimal conditions were identified as a reaction time of 190 min, a reaction temperature of 96.2 °C, and an acid (nitric acid) concentration of 6.3%. For sunflower stalks, the optimized time, temperature, and acid concentration were 130 min, 73.8 °C, and 8.7%, respectively. The pulps obtained after bleaching contained more than 90% cellulose. However, special care must be taken during the process, especially in the acid pre-treatment, as it causes the solubilization of a great amount of material. The characterization revealed that the extraction process led to cellulose pulps with around 69–70% crystallinity and thermal stability in the range of 340–350 °C, ready to be used for their conversion into derivatives for industrial applications.

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Section: Introductionmentioning

confidence: 99%

Characterization of Cellulosic Pulps Isolated from Two Widespread Agricultural Wastes: Cotton and Sunflower Stalks

La Rubia,

Jurado-Contreras,

Navas-Martos

et al. 2024

Polymers

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“…However, using increasing amounts of glucose to replace fossil-based plastics with bio-based polymers raises ethical questions due to the potential impact on food security (Mülhaupt, 2013;Walker and Rothman, 2020;Rosenboom et al, 2022). Therefore, it is of interest to explore alternative carbon sources for microbial production processes, such as lignocellulosic waste streams or plastic waste (Wierckx et al, 2015;Wendisch et al, 2016;Karp et al, 2021). Other highly promising carbon sources are C1 molecules, i.e., molecules containing only one carbon atom.…”

Section: Introductionmentioning

confidence: 99%

Engineering the methylotrophic yeast Ogataea polymorpha for lactate production from methanol

Wefelmeier,

Schmitz,

Haut

et al. 2023

Front. Bioeng. Biotechnol.

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Introduction: Lactate has gained increasing attention as a platform chemical, particularly for the production of the bioplastic poly-lactic acid (PLA). While current microbial lactate production processes primarily rely on the use of sugars as carbon sources, it is possible to envision a future where lactate can be produced from sustainable, non-food substrates. Methanol could be such a potential substrate, as it can be produced by (electro)chemical hydrogenation from CO2.Methods: In this study, the use of the methylotrophic yeast Ogataea polymorpha as a host organism for lactate production from methanol was explored. To enable lactate production in Ogataea polymorpha, four different lactate dehydrogenases were expressed under the control of the methanol-inducible MOX promoter. The L-lactate dehydrogenase of Lactobacillus helveticus performed well in the yeast, and the lactate production of this engineered strain could additionally be improved by conducting methanol fed-batch experiments in shake flasks. Further, the impact of different nitrogen sources and the resulting pH levels on production was examined more closely. In order to increase methanol assimilation of the lactate-producing strain, an adaptive laboratory evolution experiment was performed.Results and Discussion: The growth rate of the lactate-producing strain on methanol was increased by 55%, while at the same time lactate production was preserved. The highest lactate titer of 3.8 g/L in this study was obtained by cultivating this evolved strain in a methanol fed-batch experiment in shake flasks with urea as nitrogen source. This study provides a proof of principle that Ogataea polymorpha is a suitable host organism for the production of lactate using methanol as carbon source. In addition, it offers guidance for the engineering of methylotrophic organisms that produce platform chemicals from CO2-derived substrates. With reduced land use, this technology will promote the development of a sustainable industrial biotechnology in the future.

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“…In the search for innovative solutions to return wastes and by-products to the production cycle, the bioeconomy often relies on bioprocesses involving cells or their constituents. Biorefineries replace fossil carbon with renewable carbon from various lignocellulosic biomass resources to produce a range of bio-based products (Tišma et al, 2021a;Karp et al, 2021). The trend towards innovative biotechnological solutions for the valorization of lignocellulosic side-streams in the food and beverage industry is evident also from six projects funded by Bio-based Industries Joint Undertaking in 2020 (Bio-based Industries Joint Undertaking, 2020) 1 : VAMOS, HYPERBIOCOAT, IFERMENTER, VEHICLE, CAFIPLA, FIRST2RUN, and BIOSUPPACK, the latter considering also BSG.…”

Section: Introductionmentioning

confidence: 99%

The Potential of Brewer’s Spent Grain in the Circular Bioeconomy: State of the Art and Future Perspectives

Zeko-Pivač

Tišma

Žnidaršič–Plazl

et al. 2022

Front. Bioeng. Biotechnol.

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Brewer’s spent grain (BSG) accounts for approximately 85% of the total mass of solid by-products in the brewing industry and represents an important secondary raw material of future biorefineries. Currently, the main application of BSG is limited to the feed and food industry. There is a strong need to develop sustainable pretreatment and fractionation processes to obtain BSG hydrolysates that enable efficient biotransformation into biofuels, biomaterials, or biochemicals. This paper aims to provide a comprehensive insight into the availability of BSG, chemical properties, and current and potential applications juxtaposed with the existing and emerging markets of the pyramid of bio-based products in the context of sustainable and circular bioeconomy. An economic evaluation of BSG for the production of highly valuable products is presented in the context of sustainable and circular bioeconomy targeting the market of Central and Eastern European countries (BIOEAST region).

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Lignocellulosic Biorefinery for Value-Added Products: The Emerging Bioeconomy

Cited by 5 publications

References 88 publications

Characterization of Cellulosic Pulps Isolated from Two Widespread Agricultural Wastes: Cotton and Sunflower Stalks

Characterization of Cellulosic Pulps Isolated from Two Widespread Agricultural Wastes: Cotton and Sunflower Stalks

Engineering the methylotrophic yeast Ogataea polymorpha for lactate production from methanol

The Potential of Brewer’s Spent Grain in the Circular Bioeconomy: State of the Art and Future Perspectives

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