Ligustilide attenuates vascular inflammation and activates Nrf2/HO-1 induction and, NO synthesis in HUVECs

Choi, Eun Sik; Yoon, Jung Joo; Han, Byung Hyuk; Jeong, Da Hye; Lee, Yun Jung; Kang, Dae Gill; Lee, Ho Sub

doi:10.1016/j.phymed.2017.09.022

Cited by 87 publications

(51 citation statements)

References 40 publications

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“…The nuclear factor (erythroid‐derived 2)‐like 2 (Nrf2) plays a protective role against oxidative stress and inflammation. Nrf2 can promote the expression of antioxidant enzymes, such as superoxide dismutase (SOD) and catalase (CAT), which further scavenges external oxidative stress and regulates the redox balance . Nrf2 has been recognized as a major regulator of antioxidant responses, many natural compounds activate Nrf2 .…”

Section: Introductionmentioning

confidence: 99%

“…Nrf2 can promote the expression of antioxidant enzymes, such as superoxide dismutase (SOD) and catalase (CAT), which further scavenges external oxidative stress and regulates the redox balance. [9] Nrf2 has been recognized as a major regulator of antioxidant responses, many natural compounds activate Nrf2. [10] It also plays an important role in preventing the development of diabetes mellitus and the complications.…”

Section: Introductionmentioning

confidence: 99%

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Protective Effects of Anthocyanins from Coreopsis tinctoria against Oxidative Stress Induced by Hydrogen Peroxide in MIN6 Cells

Liu

Tian

et al. 2020

Chemistry & Biodiversity

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Anthocyanins (AC) from Coreopsis tinctoria possesses strong antioxidant properties, while the effects of AC on cells damage induced by reactive oxygen species (ROS) in diabetes mellitus diseases progression have not been reported. The present study was carried out to evaluate the protective property of AC against cellular oxidative stress with an experimental model, H2O2‐exposed MIN6 cells. AC could reverse the decrease of cell viability induced by H2O2 and efficiently suppressed cellular ROS production and cell apoptosis. In addition, Real‐time PCR and Western blot analyses indicated that AC could protect MIN6 cells against oxidative injury through increasing the translocation of Nrf2 into nuclear, decreasing the phosphorylation level of p38 and up‐regulating the protein expression of antioxidant enzyme (SOD1, SOD2 and CAT). Thus, this study provides evidence to support the beneficial effect of AC in inhibiting MIN6 cells from H2O2‐induced oxidative injury.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Protective Effects of Anthocyanins from Coreopsis tinctoria against Oxidative Stress Induced by Hydrogen Peroxide in MIN6 Cells

Liu

Tian

et al. 2020

Chemistry & Biodiversity

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“…The main approach against apoptosis is to prevent caspase-3 F I G U R E 1 Treatment with ligustilide (LIG) protects chondrocytes from sodium nitroprusside (SNP)-induced apoptosis. 11 LIG shows various pharmacological effects, including neuroprotective, 12 vasodilatating, 13 antitumor, 14 anti-apoptotic, 15 anti-inflammatory, 16 and antioxidant effects. B, Chondrocytes were treated with LIG in a dose-dependent manner.…”

Section: Introductionmentioning

confidence: 99%

“…10 Ligustilide (LIG; structure shown in Figure 1A) is the main lipophilic component of the Umbelliferae family, including Radix angelicae sinensis and Ligusticum chuanxiong. 11 LIG shows various pharmacological effects, including neuroprotective, 12 vasodilatating, 13 antitumor, 14 anti-apoptotic, 15 anti-inflammatory, 16 and antioxidant effects. 17 LIG, as an effective chemotherapeutic agent, attenuates IL-1β-induced inflammation and promotes extracellular matrix synthesis in chondrocytes by suppressing NF-κB activation via the PI3K/AKT pathway.…”

Section: Introductionmentioning

confidence: 99%

Ligustilide attenuates nitric oxide‐induced apoptosis in rat chondrocytes and cartilage degradation via inhibiting JNK and p38 MAPK pathways

Zhou

Jiang

et al. 2019

J Cellular Molecular Medi

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Ligustilide ( LIG ) is the main lipophilic component of the Umbelliferae family of pharmaceutical plants, including Radix angelicae sinensis and Ligusticum chuanxiong. LIG shows various pharmacological properties associated with anti‐inflammation and anti‐apoptosis in several kinds of cell lines. However, the therapeutic effects of LIG on chondrocyte apoptosis remain unknown. In this study, we investigated whether LIG had an anti‐apoptotic activity in sodium nitroprusside ( SNP )‐stimulated chondrocyte apoptosis and could delay cartilage degeneration in a surgically induced rat OA model, and elucidated the potential mechanisms. In vitro studies revealed that LIG significantly suppressed chondrocyte apoptosis and cytoskeletal remodelling, which maintained the nuclear morphology and increased the mitochondrial membrane potential. In terms of SNP , LIG treatment considerably reduced the expression levels of cleaved caspase‐3, Bax and inducible nitric oxide synthase and increased the expression level of Bcl‐2 in a dose‐dependent manner. The LIG ‐treated groups presented a significantly suppressed expression of activating transcription factor 2 and phosphorylation of Jun N‐terminal kinase ( JNK ) and p38 mitogen‐activated protein kinase ( MAPK ). The inhibitory effect of LIG was enhanced by the p38 MAPK inhibitor SB 203580 or the JNK inhibitor SP 600125 and offset by the agonist anisomycin. In vivo studies demonstrated that LIG attenuated osteoarthritic cartilage destruction by inhibiting the cartilage chondrocyte apoptosis and suppressing the phosphorylation levels of activating transcription factor 2, JNK and p38 MAPK . This result was confirmed by histological analyses, micro‐ CT , TUNEL assay and immunohistochemical analyses. Collectively, our studies indicated that LIG protected chondrocytes against SNP ‐induced apoptosis and delayed articular cartilage degeneration by suppressing JNK and p38 MAPK pathways.

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“…At present, there is still no effective Western medicine for treatment. CX, a popular Chinese medicinal herb, has multiple beneficial therapeutic properties including antioxidation (Peng et al, ), anti‐inflammation (Choi et al, ; Zhu, Zhao, Wang, Gao, & Zhang, ) and neuroprotection (Kuang et al, ) and cardiovascular effects (Hu et al, ). It is a commonly used drug for invigorating blood circulation and eliminating stasis.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous quantification of ligustilide, dl‐3‐n‐butylphthalide and senkyunolide A in rat plasma by GC–MS and its application to comparative pharmacokinetic studies of Rhizoma Chuanxiong extract alone and Baizhi Chuanxiong Decoction

Wang

Yan

Jiang

et al. 2019

Biomedical Chromatography

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The herb couple has special clinical significance in reducing the toxicity and increasing the efficacy of drugs. The combination of Radix Angelicae Dahuricae (Baizhi, BZ) andRhizoma Chuanxiong (ChuanXiong, CX) is a traditional herb couple. The combination performs better than the CX extract alone in the treatment of migraine and has been used for thousands of years. However, the specific compatibility mechanisms are still unclear. Ligustilide, DL-3-n-butylphthalide and senkyunolide A are the major active ingredients in CX and BZ-CX decoction. However, a comprehensive study of the pharmacokinetics of CX has not been carried out. A gas chromatography-mass spectroscopy (GC-MS) method with high selectivity, sensitivity and accuracy was developed. An SH-Rxi-5Sil (30 m × 0.25 mm i.d., and 0.25 μm film thickness) column was employed in the GC separation. Selectivity, linearity, precision, accuracy, recovery, matrix effect and stability were used to validate the current GC-MS method. Using the validated method, this is the first time to study on the comparative pharmacokinetics of ligustilide, DL-3-n-butylphthalide and senkyunolide A from CX alone and BZ-CX decoction in rat plasma. The pharmacokinetic parameters (C max , T max , T 1/2 , AUC 0-t , AUC 0-∞ and CLz/ F ) of all of the detected ingredients showed significant differences between the two groups (P < 0.05). The results are helpful for further investigation of the compatibility mechanism of BZ-CX decoction.

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Ligustilide attenuates vascular inflammation and activates Nrf2/HO-1 induction and, NO synthesis in HUVECs

Cited by 87 publications

References 40 publications

Protective Effects of Anthocyanins from Coreopsis tinctoria against Oxidative Stress Induced by Hydrogen Peroxide in MIN6 Cells

Protective Effects of Anthocyanins from Coreopsis tinctoria against Oxidative Stress Induced by Hydrogen Peroxide in MIN6 Cells

Ligustilide attenuates nitric oxide‐induced apoptosis in rat chondrocytes and cartilage degradation via inhibiting JNK and p38 MAPK pathways

Simultaneous quantification of ligustilide, dl‐3‐n‐butylphthalide and senkyunolide A in rat plasma by GC–MS and its application to comparative pharmacokinetic studies of Rhizoma Chuanxiong extract alone and Baizhi Chuanxiong Decoction

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