Ligustrazine ameliorates acute kidney injury through downregulation of NOD2‑mediated inflammation

Jiang, Guosheng; Xin, Rui; Yuan, Wendan; Zhang, Lixia; Meng, Xiangjun; Sun, Wangnan; Han, Hongya; Hou, Yun; Wang, Lin; Du, Pengchao

doi:10.3892/ijmm.2020.4464

Cited by 11 publications

(7 citation statements)

References 43 publications

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“…For example, IL-6, MCP-1, and TNF-α levels were upregulated by H. pylori infection in GES-1 cells (20). In the present study, H. pylori infection enhanced the mRNA and protein expression levels of IL-6/8, MCP-1, and TNF-α in GES-1 cells, which were consistent with a previous study, showing that ligustrazine reduced inflammation in the kidney cells postischemia/reperfusion injury with decreased levels of IL-6, MCP-1, and TNF-α (21). Besides, our results also confirmed that ligustrazine attenuated H. pylori-induced increased levels of IL-6/8, MCP-1, and TNF-α in GES-1 cell line, suggest-ing its anti-inflammatory effect on H. pylori-induced gastritis.…”

Section: Discussionsupporting

confidence: 93%

Ligustrazine Attenuates Gastric Inflammation and Apoptosis in Helicobacter pylori-induced Gastric Epithelial Cells

Chen

Wang

et al. 2021

Jundishapur J Microbiol

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Background: Stomach disorders, including gastric cancer and gastritis, are associated with the pathogenic bacterium Helicobacter pylori. Enhanced inflammation is the characteristic of H. pylori-induced gastritis. Ligustrazine exerts anti-inflammatory properties in mouse asthma models and acute kidney injury. Objectives: To determine the role of ligustrazine in H. pylori-induced gastritis. Methods: Normal gastric epithelial cell line (GES-1) was cultured with H. pylori at a multiplicity of infection (MOI) of 100: 1 for 24 hours. GES-1 cell line under H. pylori condition was incubated with 100 or 200 μM ligustrazine for 24 hours. Cell viability and apoptosis were investigated by MTT and flow cytometry assays, respectively. Inflammation was assessed by determining the levels and mRNA expression of interleukins (IL)-6/8, tumor necrosis factor-α (TNF-α), and monocyte chemotactic protein 1 (MCP-1) using ELISA and qRT-PCR analysis, respectively. Results: Helicobacter pylori infection reduced the viability and promoted the apoptosis of GES-1 cell line, accompanied by the enhanced activities of caspases 3 and 9. However, ligustrazine reversed the H. pylori-induced infection decreased viability, while increased apoptosis and caspases 3/9 activities in GES-1 cell line. Moreover, ligustrazine attenuated H. pylori-induced secretions of pro-inflammatory factors, IL-6/8, TNF-α, and MCP-1, in GES-1 cell line. The protein expression of inhibitor of NF-κB (IκBα) was downregulated in GES-1 cell line after H. pylori infection, while the protein expression levels of p65 and phosphorylation of IκBα were upregulated by H. pylori infection. On the contrary, ligustrazine decreased H. pylori-induced protein expression of IκBα, whereas increased protein expression of p65 and phosphorylation of IκBα. Conclusions: Ligustrazine exerted protective effects on H. pylori-induced gastric epithelial cells through inhibition of gastric inflammation and apoptosis and inactivation of NF-κB pathway.

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Section: Discussionsupporting

confidence: 93%

Ligustrazine Attenuates Gastric Inflammation and Apoptosis in Helicobacter pylori-induced Gastric Epithelial Cells

Chen

Wang

et al. 2021

Jundishapur J Microbiol

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“…Chen et al found that TMP could reduce renal I/R damage by enhancing autophagy, indicated by increased LC3-II/I ratio and Beclin-1 in kidney tissue ( Chen et al, 2020 ). Another study found that TMP reduced inflammation in renal I/R injury and was related to the activation of autophagy ( Jiang et al, 2020 ). Interestingly, in a study on CI-AKI, we found that the mechanism by which TMP protected the kidney from contrast agent damage was partly related to the inhibition of autophagy ( Gong et al, 2019 ).…”

Section: Resultsmentioning

confidence: 99%

Tetramethylpyrazine: An Active Ingredient of Chinese Herbal Medicine With Therapeutic Potential in Acute Kidney Injury and Renal Fibrosis

Gong

2022

Front. Pharmacol.

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As an increasing public health concern worldwide, acute kidney injury (AKI) is characterized by rapid deterioration of kidney function. Although continuous renal replacement therapy (CRRT) could be used to treat severe AKI, effective drug treatment methods for AKI are largely lacking. Tetramethylpyrazine (TMP) is an active ingredient of Chinese herb Ligusticum wallichii (Chuan Xiong) with antioxidant and anti-inflammatory functions. In recent years, more and more clinical and experimental studies suggest that TMP might effectively prevent AKI. The present article reviews the potential mechanisms of TMP against AKI. Through search and review, a total of 23 studies were finally included. Our results indicate that the undergoing mechanisms of TMP preventing AKI are mainly related to reducing oxidative stress injury, inhibiting inflammation, preventing apoptosis of intrinsic renal cells, and regulating autophagy. Meanwhile, given that AKI and chronic kidney disease (CKD) are very tightly linked by each other, and AKI is also an important inducement of CKD, we thus summarized the potential of TMP impeding the progression of CKD through anti-renal fibrosis.

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“…All other procedures were the same. In the I/R+TMP group, TMP hydrochloride was given immediately after reperfusion (40 mg/kg, 6-h interval) by intraperitoneal injection ( 23 ). At 24 h after the PBS cardiac perfusion, kidney tissues were taken; some tissues were stored at −80°C for protein and reverse transcription-quantitative (RT-q) PCR detection, while some tissues were fixed with 4% paraformaldehyde at 4°C for 24 h, embedded in paraffin, and sliced for morphological detection.…”

Section: Methodsmentioning

confidence: 99%

Tetramethylpyrazine alleviates acute kidney injury by inhibiting NLRP3/HIF‑1α and apoptosis

Sun

Wang

et al. 2020

Mol Med Rep

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The aim of the present study was to investigate the protective effect and underlying mechanism of tetramethylpyrazine (TMP) on renal ischemia reperfusion injury (RIRI) in rats, which refers to the injury caused by the restoration of blood supply and reperfusion of the kidney after a period of ischemia. Sprague-Dawley rats were randomly divided into a Sham group, renal ischemia-reperfusion (I/R) group and TMP group. TMP hydrochloride (40 mg/kg, 6 h intervals) was given via intraperitoneal injection immediately after reperfusion in the TMP group, after 24 h the kidney tissues were taken for follow-up experiments. Pathological changes in the kidney tissues were observed by periodic acid-Schiff staining. Renal function was assessed by measuring levels of serum creatinine and blood urea nitrogen, and inflammatory cytokines tumor necrosis factor (TNF)-α and interleukin (IL)-6. Renal cell apoptosis was detected by TUNEL-DAPI double staining, mRNA and protein changes were analyzed by reverse transcription-quantitative PCR and western blotting. Cell viability was measured using a CCK-8 assay. It was found that the renal tissues of the sham operation group were notably abnormal, and the renal tissues of the I/R group were damaged, while the renal tissues of the TMP group were less damaged compared with those of the I/R group. Compared with the I/R group, the serum creatinine and blood urea nitrogen levels in the TMP group were low (all P<0.05), levels of inflammatory cytokines TNF-α and IL-6 decreased, the apoptotic rate was low (all P<0.05), and the relative expression levels of nucleotide-oligomerization domain-like receptor 3 (NLRP3) protein and mRNA in renal tissues were low (all P<0.05). The expression levels of hypoxia-inducible factor 1-α and NLRP3 increased after oxygen and glucose deprivation (OGD), and reduced after treatment with OGD and TMP (all P<0.05). It was concluded that TMP can reduce renal injury and improve renal function in RIRI rats, and its mechanism may be related to the reduction of NLRP3 expression in renal tissues.

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Ligustrazine ameliorates acute kidney injury through downregulation of NOD2‑mediated inflammation

Cited by 11 publications

References 43 publications

Ligustrazine Attenuates Gastric Inflammation and Apoptosis in Helicobacter pylori-induced Gastric Epithelial Cells

Ligustrazine Attenuates Gastric Inflammation and Apoptosis in Helicobacter pylori-induced Gastric Epithelial Cells

Tetramethylpyrazine: An Active Ingredient of Chinese Herbal Medicine With Therapeutic Potential in Acute Kidney Injury and Renal Fibrosis

Tetramethylpyrazine alleviates acute kidney injury by inhibiting NLRP3/HIF‑1α and apoptosis

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