SummaryFor the purpose of overcoming interspecific hybrid sterility in Lilium, the following methods were attempted: 1) Further use of embryo culture in the cross of F1 and 2) Use of amphidiploids induced from F1. When F1 hybrids were used as female parents, embryos were developed in 0-3.4% of their ovules. A total of 134 seedlings from nine backcross (or the similar cross) combinations were obtained through embryo culture.A seedling from the cross of F1 as male parent flowered and proved to be a triploid.Amphidiploids could be used as pollen donors and eight B1 plants were produced from the cross of the amphidiploid of L. Ion giflorum x L. henryi.Three new interspecific hybrids in Lilium produced by embryo culture were introduced.