LIM homeodomain transcription factor Isl1 directs normal pyloric development by targeting Gata3

Li, Yushan; Pan, Jirong; Wei, Chao; Chen, Juan; Liu, Ying; Liu, Jiali; Zhang, Xiaoxin; Evans, Sylvia M.; Cui, Yan; Cui, Sheng

doi:10.1186/1741-7007-12-25

Cited by 20 publications

(25 citation statements)

References 45 publications

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“…Adult pig ovaries were embedded in paraffin and cut into 5‐μm sections. IFA of ovary sections was performed using methods described previously (Li et al, ). Antibodies against NRDR (1:100, Abcam, CA) were added to the sections and incubated at 4°C for 12 hr.…”

Section: Methodsmentioning

confidence: 99%

NRDR inhibits estradiol synthesis and is associated with changes in reproductive traits in pigs

Liu

Yang

et al. 2018

Molecular Reproduction Devel

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Cumulus cells secreting steroid hormones have important functions in oocyte development. Several members of the short‐chain dehydrogenase/reductase (SDR) family are critical to the biosynthesis of steroid hormones. NADPH‐dependent retinol dehydrogenase/reductase ( NRDR ), a member of the SDR superfamily, is overexpressed in pig breeds that also show high levels of androstenone. However, the potential functions and regulatory mechanisms of NRDR in pig ovaries have not been reported to date. The present study demonstrated that NRDR is highly expressed in pig ovaries and is specifically located in cumulus granulosa cells. Functional studies showed that NRDR inhibition increased estradiol synthesis. Both pregnant mare serum gonadotropin and human chorionic gonadotropin downregulated the expression of NRDR in pig cumulus granulosa cells. When the relationship between reproductive traits and single‐nucleotide polymorphisms (SNPs) of the NRDR gene was examined, we found that two SNPs affected reproductive traits. SNP rs701332503 was significantly associated with a decrease in the total number of piglets born during multiparity, and rs326982309 was significantly associated with an increase in the average birth weight during primiparity. Thus, NRDR has an important role in steroid hormone biosynthesis in cumulus granulosa cells, and NRDR SNPs are associated with changes in porcine reproduction traits.

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Section: Methodsmentioning

confidence: 99%

NRDR inhibits estradiol synthesis and is associated with changes in reproductive traits in pigs

Liu

Yang

et al. 2018

Molecular Reproduction Devel

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“…HRP-conjugated streptavidin (1:200; 016-030-084, Jackson) was used for incubating at RT. The diaminobenzidine (DAB) was added for color rendering the color development degree is controlled under the microscope, the appearance of brown staining was considered a positive reaction [41] Immuno uorescence When GCs grew on the glass slide and fuse to 95-100%, they are removed from the incubator and xed in 4% paraformaldehyde for 25 min. The cells were washed with PBS for 10 minutes and permeabilized with 0.1% Triton X-100 (Sigma), and then blocked using 10% normal goat serum in TBS for 1 h at room temperature.…”

Section: Immunohistochemistrymentioning

confidence: 99%

RAF1 as Downstream Molecule Mediates the FSH Signaling Pathway to Stimulate E2 Synthesis and Secretion in Mouse Ovarian Granulosa Cells

Luo

Liu

Guo

et al. 2020

Preprint

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Background: V-raf-leukemia viral oncogene 1 (RAF1) kinase is the key factor in extracellular signal regulated pathway, which transmits signals to the downstream extracellular regulated protein kinases (ERK). Regulatory function of RAF1 has been proved to mediate steroid hormone synthesis, which played an essential physiological function in reproduction and development. Whether RAF1 takes part in the signaling events of gonadotropic hormones follicle-stimulating hormone (FSH) in ovarian is unknown.Results: We found that RAF1 as downstream molecule mediates the FSH signaling pathway to stimulate estradiol (E2) synthesis and secretion in mouse ovarian granulosa cells (GCs). The expression of RAF1 is induced by FSH and the production of E2 is increased in the serum and primary ovarian GCs supernatant, the process of which is blocked by treating with RAF1 inhibitor (N-(2-Methyl-5'-morpholino-6'-((tetrahydro-2H-pyran-4-yl)oxy)-[3,3'-bipyridin]-5-yl)-3(trifluoromethyl) benzamide, RAF709). Inhibition of RAF1 activity by RAF709 decreased ERK phosphorylation, and suppressed the expression of cytochrome P450 family 19 subfamily a member 1 (CYP19A1) which is a major rate-limiting enzyme to participate in the last step of E2 biosynthesis. Conclusion: Our results suggest that RAF1 play a pivotal mediating roles toward E2 production in FSH signaling pathway by inducing the phosphorylation of ERK and promoting the process of estradiol synthesis. RAF1 may be a potential and effective factor to regulate the function of the female mouse reproductive system.

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“…Protein samples extracted from the cultured cells were subjected to 12% SDS-PAGE and immunoblotted as described previously (Li et al 2014). For SP1 analysis, the membranes were incubated with rabbit anti-SP1 antibodies (1:200; BOSTER, Wuhan, China) and mouse anti-GAPDH antibodies (1:10,000; Ambion) overnight at 4°C.…”

Section: Western Blotmentioning

confidence: 99%

miR-375 mediates CRH signaling pathway in inhibiting E2 synthesis in porcine ovary

Yu¹,

Li²,

Wang³

et al. 2017

Reproduction

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The corticotropin-releasing hormone (CRH) signaling system is involved in numbers of stress-related physiological and pathological responses，including its inhibiting effects on estradiol (E2) synthesis and follicular development in the ovary. In addition, there are reports that microRNAs (miRNAs) can control the function of animal reproductive system. The aim of present study was to investigate the functions of miR-375 and the relationship between miR-375 and CRH signaling molecules in the porcine ovary. First, our common PCR results show that miR-375 and the CRH receptor 1 (CRHR1) are expressed in porcine ovary, whereas CRH receptor 2 (CRHR2) is not detected. We further have located the cell types of miR-375 and CRHR1 by in situ hybridization (ISH), and the results show that miR-375 is located only in the granulosa cells, whereas CRHR1 is positive in all of granulosa cells and oocytes, inferring that miR-375 and CRHR1 are co-localized in granulosa cells. Second, we show that overexpression of miR-375 in cultured granulosa cells suppresses the E2 production, while miR-375 knockdown demonstrates the opposite result. Besides, our in vitro results demonstrate that miR-375 mediates the signaling pathway of CRH inhibiting E2 synthesis. Finally, our data show that the action of miR-375 is accomplished by directly binding to the 3'UTR of specificity protein1 (SP1) mRNA to decrease the SP1 protein level. Thus, we conclude that miR-375 is a key factor in regulating E2 synthesis by mediating the CRH signaling pathway.

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LIM homeodomain transcription factor Isl1 directs normal pyloric development by targeting Gata3

Cited by 20 publications

References 45 publications

NRDR inhibits estradiol synthesis and is associated with changes in reproductive traits in pigs

NRDR inhibits estradiol synthesis and is associated with changes in reproductive traits in pigs

RAF1 as Downstream Molecule Mediates the FSH Signaling Pathway to Stimulate E2 Synthesis and Secretion in Mouse Ovarian Granulosa Cells

miR-375 mediates CRH signaling pathway in inhibiting E2 synthesis in porcine ovary

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