Limit of warm ischemia time before cryopreservation in rat tracheal isografts

Nakanishi, Ryoichi; Umesue, Masayoshi; Hashimoto, Mitsunori; Muranaka, Hiroyuki; Hachida, M; Yasumoto, Kosei

doi:10.1016/s0003-4975(00)01705-7

Cited by 9 publications

(11 citation statements)

References 10 publications

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“…Based on freezing temperature and liquid nitrogen storage, cryopreserved tracheal graft protocols can be divided into two types: a) tracheal grafts frozen at -60° to -140°C and stored in liquid nitrogen until their use in surgical repair (7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19)(20), and b) tracheal grafts cryopreserved within a more homogeneous range of temperature (-80°/-85°C), without liquid nitrogen storage (3,(21)(22)(23)(24).…”

Section: Introductionmentioning

confidence: 99%

“…Most investigators agree that cryopreservation has an immunomodulating effect on tracheal allografts since it decreases antigenicity (5)(6)(7)(12)(13)(14)(25)(26)(27)(28). This is attributed to the depletion of class II leukocyte antigen expression due to the exfoliation of tracheal epithelium produced during the freezing and defrosting process (3,5,6,11,28,29).…”

Section: Introductionmentioning

confidence: 99%

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Tracheal cryopreservation: caspase-3 immunoreactivity in tracheal epithelium and in mixed glands

Sotres-Vega¹,

Baltazares-Lipp²,

Villalba-Caloca³

et al. 2009

Braz J Med Biol Res

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Cryopreservation has an immunomodulating effect on tracheal tissue as a result of class II antigen depletion due to epithelium exfoliation. However, not all epithelium is detached. We evaluated the role of apoptosis in the remaining epithelium of 30 cryopreserved tracheal grafts. Caspase-3 immunoreactivity of tracheal epithelium was studied in canine tracheal segments cryopreserved with F12K medium, with or without subsequent storage in liquid nitrogen at -196°C for 15 days. Loss of structural integrity of tracheal mixed glands was observed in all cryopreserved tracheal segments. Caspase-3 immunoreactivity in tracheal mucosa and in mixed glands was significantly decreased, in contrast to the control group and to cryopreserved tracheal segments in which it remained high, due to the effect of storage in liquid nitrogen (P < 0.05, ANOVA and Tukey test). We conclude that apoptosis can be triggered in epithelial cells during tracheal graft harvesting even prior to cryopreservation, and although the epithelial caspase-3 immunoreactivity is reduced in tracheal cryopreservation, this could be explained by increased cell death. Apoptosis cannot be stopped during tracheal cryopreservation

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Tracheal cryopreservation: caspase-3 immunoreactivity in tracheal epithelium and in mixed glands

Sotres-Vega¹,

Baltazares-Lipp²,

Villalba-Caloca³

et al. 2009

Braz J Med Biol Res

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show abstract

“…Cryopreserved tracheal allografts have been investigated for cell viability 5 and warm ischemia time 6 in animal models, and successful allotransplantation is reported in mature primates. 7 However, no experimental study regarding the growth of cryopreserved allografts has been reported.…”

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confidence: 99%

Transplantation of the cryopreserved tracheal allograft in growing rabbits

Tanaka

Murakami

Okita

2003

Journal of Pediatric Surgery

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“…We calculated the epithelial score of the graft according to the following formula: epithelial score ϭ an occupation rate of Grade 0 multiplied by 0 ϩ an occupation rate of Grade 1 multiplied by 1 ϩ an occupation rate of Grade 2 multiplied by 2 ϩ an occupation rate of Grade 3 multiplied by 3, after the ratio of occupation of a morphologically different epithelium was estimated in a cross section of the graft. 9,15,16 Cartilage viability. We calculated the ratio of chondrocytes possessing viable nuclei in the cartilage of each transplant after carefully counting the number of all chondrocytes in each high-power field on a microscopic slide.…”

Section: Morphologic Assessmentmentioning

confidence: 99%

“…A viable chondrocyte nucleus was regarded as richly stained and possessing a clear nuclear membrane. 9, 16 We then semiquantitatively graded cartilage dislocation from 0 to 3, with 0 indicating the absence of any abnormalities, 1 indicating mild changes (incidence of cartilage dislocation Ͻ30% of a microscopic visual field), 2 indicating moderate changes (30% Ͻ incidence of dislocation Ͻ 70%), and 3 indicating severe changes (incidence of dislocation Ͼ70%). 17,18 Cartilage dislocation is caused by proliferating granulation or fibrosis and has a close relationship to luminal rigidity of the graft.…”

Section: Morphologic Assessmentmentioning

confidence: 99%