Limitations of Heterologous Reagents in the Elucidation of Blood Group H(0) Specific Structures

Cross Reactive Human Blood-Group H(O) Specific Polysaccharide from Sassafras albidum and Characterization of Its Hapten*

“…Such a structure is present in L-fucose, 3-Omethyl-D-galactose, 3-O-methyl-D-fucose, and 2-0methyl-a-L-fucose. 2-0-Methyl-L-fucose is the bloodgroup H(O) specific component in the polysaccharide of Taxus cuspidata (Springer et al, 1956) and 3-Omethyl-D-fucose when present in terminal position is responsible for the blood-group H(O) activity of various cardiac glycosides and the antibiotic chartreusin (Springer and Williamson, 1963). We have been unable to determine the linkage of 3-O-methyl-D-galactose in the Sassafras macromolecule as a, 0, or both, since none of the enzymes tested released this sugar and we have found previously as also in this study that a-and /3-glycosides of O-methyl ethers of monosaccharides in the d series of fucose can both possess high and essentially equal activities (Springer et al, 1964a).…”

Immunochemical Studies on Blood Groups. XXXI. Destruction of Blood Group A Activity by an Enzyme from Clostridium tertium Which Deacetylates N-Acetylgalactosamine in Intact Blood Group Substances*

“…That the enzyme-treated A erythrocytes consistently from the serous fluid. Interpretation must be guarded because of the heterologous nature of the anti-H (O) reagents (Watkins and Morgan, 1962;Iseki and Furukawa, 1962;Springer and Williamson, 1963).…”

Synthesis and Immunochemistry of Fucose Methyl Ethers and Their Methylglycosides*

“…Final concentrations of inhibitor are obtained by dividing the given values by 3. Table II lists the effectiveness in the hemagglutination-inhibition assays of the active l-and D-fucose methyl ethers and their methylglycosides as measured with eel serum and Lotus agglutinins. For comparison L-fucose, its methylglycopyranosides, and previously described blood-group active macromolecules (Springer and Williamson, 1963) are also included. It can be readily seen that a number of methyl ethers were as active or even more so than L-fucose when determined with eel serum.…”