Limitations of PCR‐based molecular markers to identify male‐sterile and maintainer plants from Indian onion (<i>Allium cepa</i> L.) populations

Khar, Anil; Saini, Neeru

doi:10.1111/pbr.12373

Cited by 25 publications

(22 citation statements)

References 26 publications

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“…Despite the advance in the cytoplasmic identification, the identification of the 'B' line was dependent on manual backcrosses in the field. Identification of the cytoplasm type was performed by Ragassi et al (2010) and Santos et al (2012), in predominantly Brazilian germplasm, and by Khar and Saini (2016) in Indian germplasm. Huo et al (2015) and Kim et al (2015) reported the development of markers for identification of the male-sterility restorer nuclear locus (Ms) making it possible to fully identify 'A' and 'B' lines assisted by markers.…”

Section: Introductionmentioning

confidence: 99%

Fertility restoration locus and cytoplasm types in onion

Ferreira¹,

Santos²,

Oliveira³

2017

gmr

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The objective of this study was the identification of the cytoplasmic types and the genotyping for the fertility restoration nuclear locus (Ms) in 59 onion accessions, aiming at the selection of 'A' and 'B' lines essential for the obtainment of hybrids. Three markers were used to identify the cytoplasm 5' cob, orfA501, and orf725, and two were used for the Ms locus (AcSKP1 and AcPMS1). The two types of male-sterile cytoplasm ('S' and 'T'), as well as fertile cytoplasm ('N'), and the Ms and ms alleles in both homozygosity and heterozygosity were detected in the 59 genotypes evaluated in the experiment. The frequencies of the 5' cob/orfA501 and orf725 markers, as well as of the markers AcSKP1 and AcPMS1, were close in the onion accessions evaluated in this study. In the Brazilian germplasm, the frequencies of the 'N', 'S', and 'T' cytoplasm were approximately 0.47, 0.28, and 0.25, respectively, whereas the allele frequencies of Ms and ms were 0.52 and 0.48, respectively. The accessions Régia, EHCEB 20146, EHCEB 201427, Alvorada, Serrana, Crioula Mercosul, EHCEB 20142, BRS 367, Rainha, Juporanga, and Alfa SF C-XI have potential for the identification of 'A' and 'B' lines, since they presented mixtures of cytoplasm and different allele frequencies for Ms. All the plants of the accessions EHCEB 20142040/EHCEB 20141040, EHCEB 20142028/EHCEB 20141028, and EHCEB 20112006/EHCEB 20111006 were in the Nmsms and Smsms conditions, and have the potential for 'B' and 'A' lines, respectively, for the CMS-S system. All the plants of the accessions EHCEB 20142027/EHCEB 20141027, EHCEB 20102019/EHCEB 20101019, and Alfa SF 'B'/Alfa SF 'A' were in Nmsms and Tmsms conditions, and have the potential for 'B' and 'A' lines, respectively, for the CMS-T system.

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Section: Introductionmentioning

confidence: 99%

Fertility restoration locus and cytoplasm types in onion

Ferreira¹,

Santos²,

Oliveira³

2017

gmr

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“…The accD cytoplasmic marker together with the AcPms1 nuclear marker correctly predicted male sterility across all three populations, although not necessarily male fertility restoration. Although Khar and Saini (2016) observed that these markers were not completely accurate in germplasm from the Indian subcontinent, we can recommend their use for marker-aided selection of maintainer lines for onion populations of European or North American origins (Havey and von Kohn, 2017) toward the development of male sterile inbred lines for the production of onion hybrids. Means followed by the same letter in parentheses in columns and rows were not significantly different using Tukey's honestly significant difference test at P = 0.05.…”

Section: Resultsmentioning

confidence: 92%

Variable Penetrance among Different Sources of the Male Fertility Restoration Allele of Onion

HsiangI¹,

Havey²

2020

horts

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Hybrid onion (Allium cepa) seed is produced using cytoplasmic male sterility (CMS). For the most commonly used source of onion CMS, male fertile plants possess male sterile (S) cytoplasm and dominant allele(s) at one nuclear male fertility locus (Ms). Because male fertility restoration is not necessary for bulb production, it is desirable to purge dominant alleles at Ms from populations and breeding lines to facilitate the development of male sterile inbreds for hybrid production. In this research, we used molecular markers to establish the cytoplasms and genotypes at Ms in progenies from testcrosses of male sterile lines with plants from three populations [B2354, Ailsa Craig (AC), and Sapporo-Ki (Ski)] possessing the dominant Ms allele. We scored male fertility of testcross progenies by visual examination of flowers and acetocarmine staining of pollen. Different sources of the dominant Ms allele showed significantly different amounts of male fertility restoration and proportions of stainable pollen, complicating visual selection against the dominant Ms allele. For AC and Ski, molecular markers correctly predicted male sterility vs. male fertility of progenies in the greenhouse and field. However, for B2354, male fertility restoration was less clear and especially difficult to score under field conditions, consistent with reduced penetrance of male fertility restoration for this source of the dominant Ms allele. These results will be of interest to onion breeders selecting S-cytoplasmic male sterile lines for hybrid onion development.

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“…), but their validation is needed before applying them in MAS of maintainer lines (Saini et al. , Khar and Saini ).…”

Section: Pollination Control Methods In Different Vegetable Cropsmentioning

confidence: 99%

“…Several molecular markers have been developed to identify the different cytoplasm types, thus reducing time and labour (Havey 1995, Sato 1998, Engelke et al 2003, Cho et al 2006, Kohn et al 2013. Likewise, many attempts have been made to develop molecular markers tightly linked to the Ms locus (G€ okc ße et al 2002, Bang et al 2011, Huo et al 2012, Yang et al 2013, Havey 2013, Huo et al 2015, Kim et al 2015, but their validation is needed before applying them in MAS of maintainer lines (Saini et al 2015, Khar andSaini 2016).…”

Section: Amaryllidaceaementioning

confidence: 99%

The use of genetic, manual and chemical methods to control pollination in vegetable hybrid seed production: a review

Colombo¹,

Galmarini

2017

Plant Breeding

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Production of hybrid varieties of vegetable crops is currently a desired breeding goal, due to their remarkable agronomic performance and to the possibility of intellectual property protection. However, efficient hybrid production requires a careful pollination control to guarantee the hybrid nature of F 1 seed. Several technologies ranging from manual emasculation to genetic transformation are used to inhibit pollen production in mother plants. In this review, we examine the principles underlying strategies like genetically determined systems (genic male sterility, cytoplasmic-genic male sterility, self-incompatibility) and other methods (manual emasculation, chemical-hybridizing agents) in different species, considering the benefits and drawbacks of their adoption. Finally, we present the current state of the art for vegetable hybrid seed production.

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Limitations of PCR‐based molecular markers to identify male‐sterile and maintainer plants from Indian onion (Allium cepa L.) populations

Cited by 25 publications

References 26 publications

Fertility restoration locus and cytoplasm types in onion

Fertility restoration locus and cytoplasm types in onion

Variable Penetrance among Different Sources of the Male Fertility Restoration Allele of Onion

The use of genetic, manual and chemical methods to control pollination in vegetable hybrid seed production: a review

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