The development of a resilient and circular bio-based economy is of paramount importance, notably in the EU, where current climate policies and evolving regulations strongly demand more sustainable practices, impacting monitoring and reporting, as well as the deployment of novel valorization routes for byproducts and waste streams. In this context, with the aim of assessing the current state of the European bio-based economy, a comprehensive analysis based on socio-environmental, socioeconomic, and technical indicators was carried out on major sectors, namely textiles, woodworking, pulp and paper, bio-based chemicals and materials, liquid biofuels, and bio-based electricity. Each sector was evaluated with respect to its main biological raw materials, and a methodology is proposed to link their geographical origin (inside or outside the EU), import shares, and internal production with socio-environmental impacts, based on official databases and indexes. Socioeconomic data (turnover and employment) and technical data (average bio-based content within the main products of the sector) were also considered for the analyses, allowing a multi-angle comparison between sectors and the identification of barriers and opportunities for future developments. Finally, a quantitative and qualitative overview of non-hazardous biogenic waste streams generated in the EU is presented, and opportunities for their valorization and reintegration into the EU bio-based economy are discussed. As a result of this analysis, beyond enabling the assessment of each sector within the bio-based economy, along with the assignment of values for comparison, the implementation of this evaluation facilitated the identification of improvement pathways, which were consolidated into a set of proposals.