Limited Additional Release of Cardiac Troponin I and T in Isoproterenol-Treated Beagle Dogs with Cardiac Injury

Feng, Xiao; Taggart, Peter; Hall, L. Mark; Bryant, Stewart; Sansone, Joseph; Kemmerer, Matthias; Herlich, Judy; Lord, Peter G.

doi:10.1373/clinchem.2005.049643

Cited by 10 publications

(9 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In acute myocardial injury in dogs, cTnI increases within 4 hours of myocardial ischemia and necrosis, reaches peak concentrations in 8 hours, and remains persistently increased for more than 50 hours. 46 Echocardiographic abnormalities, which have not been described previously in dogs with ehrlichiosis, were detected in more than one-third of E. canis-infected dogs in this study. Left ventricular dilation and hypertrophy were associated with myocardial damage.…”

Section: Discussionsupporting

confidence: 53%

See 1 more Smart Citation

Serum Cardiac Troponin I Concentration in Dogs with Ehrlichiosis

Diniz

Morais

Breitschwerdt

et al. 2008

Veterinary Internal Medicne

View full text Add to dashboard Cite

Background: Ehrlichiosis is a multisystemic disease with the potential to cause cardiomyocyte injury in naturally infected dogs.Hypothesis: Myocardial injury occurs in dogs infected with Ehrlichia canis. Animals: One-hundred and ninety-four dogs from Brazil with clinical and laboratory abnormalities indicative of ehrlichiosis. Sixteen healthy dogs served as controls.Methods: Electrocardiogram, echocardiogram, noninvasive blood pressure measurement, and serum cardiac troponin I (cTnI) concentrations were evaluated. Serologic assays and PCR determined the exposure and infection status for E. canis, Anaplasma spp., Babesia canis vogeli, Bartonella spp., Borrelia burgdorferi, Dirofilaria immitis, Ehrlichia chaffeensis, Ehrlichia ewingii, Leishmania chagasi, and spotted-fever group Rickettsia. Dogs were assigned to groups according to PCR status: E. canis infected, infected with other vector-borne organisms, sick dogs lacking PCR evidence for infection, and healthy controls.Results: E. canis-infected dogs had higher serum cTnI concentrations than controls (median: 0.04 ng/dL; range 0.04-9.12 ng/dL; control median: 0.04 ng/dL; range: 0.04-0.10 ng/dL; P 5 .012), and acute E. canis infection was associated with myocardial injury (odds ratio [OR]: 2.67, confidence interval [CI] 95%: 1.12-6.40, P 5 .027). Severity of anemia was correlated with increased risk of cardiomyocyte damage (r 5 0.84, P o .001). Dogs with clinical signs of systemic inflammatory response syndrome (SIRS) were at higher risk for myocardial injury than were other sick dogs (OR: 2.55, CI 95%: 1.31-4.95, P 5 .005).Conclusions and Clinical Importance: Acute infection with E. canis is a risk factor for myocardial injury in naturally infected Brazilian dogs. Severity of anemia and SIRS might contribute to the pathophysiology of myocardial damage.

show abstract

Section: Discussionsupporting

confidence: 53%

“…The kinetics of cTnI in dogs may potentially explain these findings. In acute myocardial injury in dogs, cTnI increases within 4 hours of myocardial ischemia and necrosis, reaches peak concentrations in 8 hours, and remains persistently increased for more than 50 hours 46 …”

Section: Discussionmentioning

confidence: 99%

Serum Cardiac Troponin I Concentration in Dogs with Ehrlichiosis

Diniz

Morais

Breitschwerdt

et al. 2008

Veterinary Internal Medicne

View full text Add to dashboard Cite

show abstract

“…Measurement of cardiac troponin (cTn) concentration in serum or plasma to diagnose and monitor cardiac injury is an accepted practice in human and veterinary medicine (Adin et al 2006;Burgener et al 2006;Collinson, Boa, and Gaze 2001;Feng et al 2005;Gaze and Collinson 2005;Herman et al 2006;O'Brien 2006;O'Brien et al 2006). Although initially employed as the gold standard for the diagnosis of acute coronary syndrome and myocardial infarction in people (Antman et al 2000), quantitation of this intracellular globular protein released from injured cardiac myocytes has proven helpful in the diagnosis of cardiac injury due to a variety of causes (NACB Writing Group et al 2007;O'Brien 2006).…”

Section: Introductionmentioning

confidence: 99%

A Comparison of Mortality and Cardiac Biomarker Response between Three Outbred Stocks of Sprague Dawley Rats Treated with Isoproterenol

et al. 2011

View full text Add to dashboard Cite

The authors compared the mortality and cardiac biomarker responses in three outbred stocks of Sprague Dawley rats (CD/IGS, Sasco, Harlan) treated with isoproterenol hydrochloride. Cardiac injury was confirmed by histologic evaluation, and increases in cardiac troponin I concentration in serum were measured by two methods. CD/IGS rats had a higher incidence and earlier mortality compared with Sasco or Harlan rats. Harlan rats had lower severity scores for cardiomyocyte degeneration/necrosis compared with the other stocks. Post-isoproterenol treatment cardiac troponin I concentrations were greater in CD/IGS and Sasco rats compared with Harlan rats. Concentrations of cardiac troponin T followed a similar pattern to that of cardiac troponin I in rats treated with isoproterenol. Myosin, light chain 3 concentrations increased in all rats treated with isoproterenol, but there was no difference between the three stocks in the magnitude or pattern of the dose response. Increases in fatty acid binding protein 3 concentrations were detected in only the highest dose group at the earliest timepoint postdose for all three stocks of rats. Results of these studies illustrate the need for investigators to recognize the potential differences in response between stocks of Sprague Dawley rats treated with cardiotoxicants or novel chemical entities.

show abstract

“…Blood cardiac troponin-I (cTnI) concentration has been validated as a biomarker for cardiotoxicity in numerous animal models, including rat, dog, and monkey (Adin et al 2006;Burgener et al 2006;Feng et al 2005;Gaze and Collinson 2005;Herman et al 2006;O'Brien 2006;O'Brien et al 2006). Currently a wide variety of immunoassays and platforms are available to measure human cTnI concentrations; however, little has been done to validate these systems with regard to sensitivity across species using an internationally recognized standard (Adin et al 2006;Peetz et al 2006;Storti et al 2006).…”

Section: Introductionmentioning

confidence: 99%

Ultrasensitive Cross-species Measurement of Cardiac Troponin-I Using the Erenna Immunoassay System

et al. 2008

View full text Add to dashboard Cite

Serum cardiac troponin-I (cTnI) has been validated as a biomarker for cardiotoxicity in numerous animal models; however, owing to sensitivity issues cTnI concentrations in healthy, resting animals used in toxicology studies have not been established. Serum from healthy and isoproterenol hydrochloride (iso)-treated rats, dogs, and monkeys were assayed using the Erenna system. The Erenna cTnI assay provided sensitivity < 1 ng/L across human, rat, dog, and monkey cTnI. Linear responses (R 2 = 0.99) were observed for all species. Precision studies yielded interassay CVs of curve fit quantification from 2% to 4% between 1.6 and 5000 ng/L, and 23% at 0.78 ng/L. Strong correlation (R 2 = 0.99) was obtained between Erenna and Beckman Access cTnI. Concentrations of cTnI in healthy animals ranged from 1 to 9 ng/L. In longitudinal studies of iso-treated animals, the concentrations of cTnI in the control vehicle-treated groups were 10-20 ng/L for rats (N = 10) and predose values of 2-3 ng/L for dogs (N = 3). Measured with the Erenna assay system, cTnI was quantifiable at all time intervals tested in all animals treated with iso. The Erenna system provides sensitive measurement of cTnI in rats, dogs, and monkeys, makes it possible to determine small changes from normal concentrations, and provides cTnI values from small volumes of serum.

show abstract

Limited Additional Release of Cardiac Troponin I and T in Isoproterenol-Treated Beagle Dogs with Cardiac Injury

Cited by 10 publications

References 9 publications

Serum Cardiac Troponin I Concentration in Dogs with Ehrlichiosis

Serum Cardiac Troponin I Concentration in Dogs with Ehrlichiosis

A Comparison of Mortality and Cardiac Biomarker Response between Three Outbred Stocks of Sprague Dawley Rats Treated with Isoproterenol

Ultrasensitive Cross-species Measurement of Cardiac Troponin-I Using the Erenna Immunoassay System

Contact Info

Product

Resources

About