Limited contribution of hatchery-produced individuals to the sustainment of wild marble trout (Salmo marmoratus Cuvier, 1829) in an Alpine basin

Righi, Tommaso; Fasola, Emanuele; Iaia, Mattia; Stefani, Fabrizio; Volta, Pietro

doi:10.1016/j.scitotenv.2023.164555

Cited by 4 publications

(3 citation statements)

References 111 publications

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“…The mitochondrial control region (CR) was PCR amplified using the primers 28RIBa (Sušnik et al, 2001) and HN20 (Bernatchez & Danzmann, 1993), following procedures described in Bernatchez and Danzmann (1993). Single-strand conformation polymorphisms (SSCP) (Orita et al, 1989) were analysed following the method reported in Righi et al (2023). Sanger sequencing of the CR ($1 Kbs) was performed, using the same primers of amplification, on a subsample for each different SSCP detected profile on an Applied Biosystems ABI 3730XL DNA by a service facility (BMR-Genomic, Padua).…”

Section: Mitochondrial Dnamentioning

confidence: 99%

Population genetics, demography and conservation of Mediterranean brown trout from Sardinia

Splendiani,

Righi,

Fioravanti

et al. 2024

Aquatic Conservation

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Brown trout is a species complex (Salmo trutta complex, L., 1758) including both widespread invasive (non‐native hatchery strains) lineages and endangered local‐endemic lineages, among which is the Sardinian trout, the only native salmonid present in Sardinia. Multiple stressors (e.g. the spread of stocked brown trout of Atlantic origin, habitat alteration and climate change) combine to seriously threaten the persistence of wild native populations. In this study, the origin, population genetics and demography of wild Sardinian brown trout populations were extensively investigated. A total of 274 trout individuals collected from 12 hydro‐geographical basins were analysed using both mitochondrial (control region) and nuclear (LDH‐C1* locus and 10 microsatellites) markers. Although stocking activities have altered the native genetic makeup of some populations in the study area, several (almost) uncontaminated populations showing strong genetic structure were detected. Eroded intra‐population diversity, as well as small effective population size, sometimes associated with a bottleneck signal was also found. The genetic characteristics of Sardinian trout populations described in this study are probably due, at least partly, to the peculiarity of local environmental conditions at the margin of the ecological niche for salmonids. Based on the results of this study, the need for urgent measures of conservation aimed to ensure the near future viability of the last wild Sardinian trout populations was discussed.

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Section: Mitochondrial Dnamentioning

confidence: 99%

Population genetics, demography and conservation of Mediterranean brown trout from Sardinia

Splendiani,

Righi,

Fioravanti

et al. 2024

Aquatic Conservation

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“…The DA lineage inhabits the northern, eastern, and central parts of the Balkan Peninsula, i.e., the Black Sea basin, and the AD lineage inhabits the northwestern and southwestern regions, including numerous lotic and some lentic habitats in the continental part of the Adriatic and Ionian Seas' basins (Georgiev, 2003). The MA lineage is found close to the dispersal range of brown trout of the Danubian and Adriatic lineages, in the narrowest area in the Western Balkans, inhabiting the Adriatic drainages in Bosnia and Herzegovina and Montenegro (Snoj et al, 2010;Mrdak eta al., 2012;Škraba Jurlina et al, 2020;Righi et al, 2023). The status of the MA lineage, which is still often identified as marble trout, has not yet been resolved in Croatia (Ćaleta et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

“…The loss of these unique populations could lead to a significant reduction in genetic variability within the species (Laikre et al, 1999). Stocking became a common practice to increase and support wild stocks of many fish species, including brown trout (Righi et al, 2023). In the beginning of the 20th century, it was pointed out that supportive breeding programs could also pose a threat to gene-level biodiversity, by releasing cultured individuals into the wild, with the aim of increasing the size of natural populations (Laikre et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Loss of native brown trout diversity in streams of the continental Croatia

Kanjuh,

Marić,

Škraba Jurlina

et al. 2024

Front. Environ. Sci.

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Introduction: The genetic diversity of brown trout in the Western Balkans has been disrupted by the introduction of non-native Atlantic phylogenetic lineages and non-native haplotypes of the Danubian phylogenetic lineage. The Western Balkans is characterized by the greatest phenotypic and genotypic diversity of trout populations, and a large part of the internal territory belongs to the Black Sea basin, where the Danubian Da1 haplotype is native. Artificial propagation of non-native lineages in the Western Balkans has a long history, and these populations are often the only available material for stocking rivers attractive for fishing.Material and Methods: Fifteen populations in the Danube basin of the continental Croatia were analysed. The analysis of eight microsatellite loci was performed to determine the structure of brown trout populations, as well as the degree of introgression of non-native genetic material into the native.Results and Disscusion: The results of this study showed significant genetic similarity among brown trout populations, confirming a long history of introduction with non-native genetic material. The main reason was uncontrolled stocking with inadequate material, which is available in fish farms and consists mainly of brown trout of the Atlantic phylogenetic lineage. The results of this study also indicated stocking with brown trout of the non-native haplotypes of the Danubian phylogenetic lineage. The potential breeding origin of brown trout carrying the Danubian Da2 mtDNA haplotype and ways of its introduction into rivers have yet to be investigated. For the survival of the unique gene pool of brown trout in Croatian rivers, it is of fundamental importance to know the structure of wild and farmed populations with the aim of proposing and implementing conservation measures.

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Population-genetics analysis of the brown trout broodstock in the “Panjica” hatchery (Serbia) and its conservation applications

Veličković,

Snoj,

Bravničar

et al. 2024

Knowl. Manag. Aquat. Ecosyst.

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Artificial propagation and stocking of brown trout is a standard practice in recreational fishery management. In recent decades, the importance of maintaining intraspecific diversity and protecting locally adapted lineages has been recognized for the species' long-term survival. The first step in selecting donors for stocking involves distinguishing native trout from non-native and introgressed individuals. The established method for discerning Atlantic hatchery strains from the wild populations involves genetic screening of individual diagnostic SNPs and microsatellite assignment tests. This study, using Serbia's Panjica hatchery as an example, illustrates the proper conduct of routine genetic screening for identifying suitable donors for supportive stocking. The broodstock and reference populations were screened using mtDNA control region, LDH nuclear gene, and 12 microsatellite loci to assess the origin, diversity, and inbreeding levels. The analysis revealed only moderate contamination with Atlantic trout and showed the regional origin of the Danubian genes – over 50% of the broodstock was composed of non-introgressed Danubian individuals tracing their origin to the Zapadna Morava River system. Additionally, the study highlighted a considerable discordance between LDH locus and microsatellites in identifying introgressed individuals, raising concerns about the sole reliance on LDH locus for the identification of Atlantic genetic origin in nuclear DNA.

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Limited contribution of hatchery-produced individuals to the sustainment of wild marble trout (Salmo marmoratus Cuvier, 1829) in an Alpine basin

Cited by 4 publications

References 111 publications

Population genetics, demography and conservation of Mediterranean brown trout from Sardinia

Population genetics, demography and conservation of Mediterranean brown trout from Sardinia

Loss of native brown trout diversity in streams of the continental Croatia

Population-genetics analysis of the brown trout broodstock in the “Panjica” hatchery (Serbia) and its conservation applications

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