Limited effects of combined dietary copper deficiency/iron overload on oxidative stress parameters in rat liver and plasma

Cockell, Kevin A.; Wotherspoon, Andrew T.L.; Belonje, B.; Fritz, Melissa; Madère, René; Hidiroglou, Nick; Plouffe, Louise J.; Ratnayake, W. M. N.; Kubow, Stan

doi:10.1016/j.jnutbio.2005.04.003

Cited by 22 publications

(15 citation statements)

References 42 publications

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“…Total plasma GSH was determined by HPLC using a manual adaptation of the automated NaBH 4 reduction and monobromobimane derivatization procedures described previously [31,32]. Liver cytosolic and plasma protein carbonyls were determined by slot-blot immunoassay using reagents from an OxyBlot Protein Oxidation Detection Kit (Intergen, NY, USA) as previously described [33]. Lipid peroxide concentrations in liver homogenates and plasma were determined using a commercially available kit (LPO-CC Lipid Peroxides, Kamiya Biomedical, Seattle, WA, USA).…”

Section: Methodsmentioning

confidence: 99%

Sparing effects of selenium and ascorbic acid on vitamin C and E in guinea pig tissues

Bertinato

Hidiroglou

Peace

et al. 2007

Nutr J

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Section: Methodsmentioning

confidence: 99%

Sparing effects of selenium and ascorbic acid on vitamin C and E in guinea pig tissues

Bertinato

Hidiroglou

Peace

et al. 2007

Nutr J

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“…Protein carbonyls were measured in plasma by a dot-blot immunoassay, following dinitrophenyl hydrazine reaction of protein carbonyl groups, using commercially available primary antibody to the resulting dinitrophenyl (DNP) derivatives [primary rabbit anti-DNP and horseradish peroxidase (HRP)-conjugated secondary goat anti-rabbit], followed by ECLϩ and chemiluminescence detection (20). Plasma samples used for analysis of protein carbonyls were selected from those infants whose urine had the highest and lowest urinary isoprostane values to determine whether carbonyl content reflected isoprostane values and if further analysis should be done on the entire urine sample collection.…”

Section: Subjectsmentioning

confidence: 99%

Evidence of Oxidative Stress in Relation to Feeding Type During Early Life in Premature Infants

et al. 2011

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Morbidity in the premature (PT) infant may reflect difficult adaptation to oxygen. We hypothesized that feeding including formula feeding (F) and feeding mother's milk (HM) with added fortifier would affect redox status. Therefore, 65 PT infants (birth weight: 1146 Ϯ 261 g; GA: 29 Ϯ 2.5 wk; mean Ϯ SD) were followed biweekly, once oral feeds were introduced. Feeding groups: F (Ͼ75% total feeds) and HM (Ͼ75% total feeds) were further subdivided according to human milk fortifier (HMF) content of 0 -19, 20 -49, and Ն50%. Oxidative stress was quantified by F2-isoprostanes (F2-IsoPs) in urine, protein carbonyls, and oxygen radical absorbance capacity (ORAC) in plasma.

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“…Oxysterol determination of hepatic tissues was performed by GC-MS as described previously 59. Briefly, 19-hydroxycholesterol was added to the samples as an internal standard before lipid extraction.…”

Section: Assaysmentioning

confidence: 99%

Antioxidant Supplements Improve Profiles of Hepatic Oxysterols and Plasma Lipids in Butter-fed Hamsters

Poirier

Cockell

Ratnayake

et al. 2010

Nutr Metab Insights

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Hypercholesterolemic diets are associated with oxidative stress that may contribute to hypercholesterolemia by adversely affecting enzymatically-generated oxysterols involved in cholesterol homeostasis. An experiment was conducted to examine whether the cholesterol-lowering effects of the antioxidants selenium and α-tocopherol were related to hepatic oxysterol concentrations. Four groups of male Syrian hamsters (n = 7–8) were fed high cholesterol and saturated fat (0.46% cholesterol, 14.3% fat) hypercholesterolemic semi-purified diets: 1) Control; 2) Control + α-tocopherol (67 IU all-racemic-α-tocopheryl-acetate/kg diet); 3) Control + selenium (3.4 mg selenate/kg diet); and 4) Control + α-tocopherol + selenium. Antioxidant supplementation was associated with lowered plasma cholesterol concentrations, decreased tissue lipid peroxidation and higher hepatic oxysterol concentrations. A second experiment examined the effect of graded selenium doses (0.15, 0.85, 1.7 and 3.4 mg selenate/kg diet) on mRNA expression of the oxysterol-generating enzyme, hepatic 27-hydroxylase (CYP27A1, EC 1.14.13.15), in hamsters (n = 8–9) fed the hypercholesterolemic diets. Supplementation of selenium at 3.4 mg selenate/kg diet was not associated with increased hepatic 27-hydroxylase mRNA. In conclusion, the cholesterol lowering effects of selenium and α-tocopherol were associated with increased hepatic enzymatically generated oxysterol concentrations, which appears to be mediated via improved antioxidant status rather than increased enzymatic production.

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Limited effects of combined dietary copper deficiency/iron overload on oxidative stress parameters in rat liver and plasma

Cited by 22 publications

References 42 publications

Sparing effects of selenium and ascorbic acid on vitamin C and E in guinea pig tissues

Sparing effects of selenium and ascorbic acid on vitamin C and E in guinea pig tissues

Evidence of Oxidative Stress in Relation to Feeding Type During Early Life in Premature Infants

Antioxidant Supplements Improve Profiles of Hepatic Oxysterols and Plasma Lipids in Butter-fed Hamsters

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