Limited fibrinogenolysis in stored whole blood and in fresh frozen plasma

Klaubert, W.; Schulte-Derne, O; Gollwitzer, Rotraut; Mempel, W; Wilmanns, W.

doi:10.1016/0049-3848(88)90173-9

Cited by 3 publications

(6 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Previous studies of the effect of storage on fibrinogen level have presented disparate results. For example, Nilsson et al 22 reported no change in fibrinogen level and fibrinolytic activity during the storage period, whereas Klaubert et al 23 reported a clear increase in fibrinolytic activity and subsequent production of the fibrinogen α‐chain fragment F‐CB3, concomitant with a decrease in fibrinogen level. Fragmentation of the fibrinogen molecule (as indicated by the appearance of F‐CB3), in addition to the decrease in its level, might reduce its aggregating capacity.…”

Section: Resultsmentioning

confidence: 99%

Alteration of red cell aggregability and shape during blood storage

Hovav¹,

Yedgar

Manny³

et al. 1999

Transfusion

162

125

View full text Add to dashboard Cite

show abstract

Section: Resultsmentioning

confidence: 99%

Alteration of red cell aggregability and shape during blood storage

Hovav¹,

Yedgar

Manny³

et al. 1999

Transfusion

162

125

View full text Add to dashboard Cite

show abstract

“…Weitz et al [ 15] reported that leukocyte-elastase-mediated fibrinogenolysis occurs in the presence of phy siological concentrations of antiproteinase. Klaubert et al [16] suggested that liberation of leukocyte elas tase increases in whole blood during storage and that limited fibrinogenolysis takes place and still clottable fibrinogen degradation fragments are observed early.…”

Section: Discussionmentioning

confidence: 99%

Role of D Dimer in Patients with Elevated Fibrinogen Degradation Products in Serum: Further Study in Chronic Myelogenous Leukemia

Saitô¹,

Asakura²,

Jokaji³

et al. 1990

Acta Haematol

View full text Add to dashboard Cite

We quantitatively assayed the levels of cross-linked fibrin degradation products (D dimer) in plasma at 73 points in time in 32 patients with elevated fibrin/fibrinogen degradation products (FDP) in serum. The assay of FDP was performed on serum samples prepared in test tubes containing 5 U/ml thrombin (final concentration) by a method based on latex agglutination using antifibrinogen antibodies, and the levels of D dimer in plasma were determined by a newly developed latex immunoassay using monoclonal antibodies which do not cross-react with fibrinogen. 5 patients with chronic myelogenous leukemia (CML) had highly elevated FDP levels with normal levels of D dimer. Plasma samples from such patients with CML were treated with various concentrations of thrombin (2–10 U/ml) and after the removal of fibrin clots the levels of FDP in supernatants were assayed by the FDP assay procedure described above. The levels of FDP were normalized when plasma were treated with 10 U/ml thrombin. In 2 patients with CML who had elevated levels of FDP in serum, it was impossible to remove fibrinogen completely by addition of 5 U/ml thrombin. However, FDP levels in the sera treated with 5 U/ml thrombin were almost normal in normal controls and patients with other diseases than CML. From these results it is concluded that residual fibrinogen reacted in the assay procedure as markedly increased FDP in supernatants and elevated FDP levels in serum reflected fibrinogen-related materials, which may not completely polymerize in the presence of lower concentrations of thrombin in some patients with CML. The assay of D dimer in plasma using monoclonal antibodies is recommended in cases of CML to rule out disseminated intravascular coagulation.

show abstract

“…If the oxidized form of CoQ10 in the electron transport chain structure is suppressed by antioxidants, this may provide a protection mechanism for the mitochondria. Under normal conditions, blood can be safely stored for about a month at +4 0 C and longer period of stored blood can not be used (1). This situation causes an additional financial burden due to the disposal of the blood bags in which the stored blood is preserved.…”

Section: Introductionmentioning

confidence: 99%

“…Consequently, the formed free radicals cause lipid, protein and DNA oxidation, which are all structural membrane components, cellular elements, core proteins and nucleic acids in stored blood (2,4). Therefore, immediately some changes begin to occur in the stored blood cells starting from the initial time of blood storage (1). When there are at least three double bonds in lipid fatty acids, malondialdehyde (MDA) occur as end product in the result of lipid peroxidation (5).…”

Section: Introductionmentioning

confidence: 99%