Limited genotypic and geographic variability of 16-O-methylated diterpene content in Coffea arabica green beans

Genetic‐environment interactions and climatic variables effect on bean physical characteristics and chemical composition of Coffea arabica

“…Fatty acid methyl esters were purified and detected using an Agilent 7890 gas chromatograph equipped with a DB‐23 column (60 cm × 0.25 mm × 0.25 μm; Agilent Technologies, Wilmington, DE, USA) and flame ionization. Fatty acids were identified by comparing their retention time with commercial fatty acid standards (Sigma‐Aldrich) and quantified using ChemStation (Agilent) to calculate the peak surfaces as previously described 43,44 …”

“…Ultra‐performance liquid chromatography–tandem mass spectrometry (UPLC–MS/MS) was performed with an Agilent 1290 UPLC interfaced to a SCIEX Triple Quad™ 4500, using a chromatography column Waters ACQUITY HSS T3 C18 (2.1 mm × 100 mm, particle size 1.8 μm) maintained at 30 °C with a flow of 500 μL min −1 . A two solvent system was used: solvent A, 0.1% formic acid in H 2 O; solvent B, acetonitrile; with an injection volume of 10 μL and a linear gradient as follows: 0 min 40% A; 6.5 min 40% A; 9.5 min 20% A; 10 min 40% A. Cafestol and kawheol were quantified by integration of corresponding peaks, respectively, at 5.82 ppm, 6.14 ppm, and 3.10 ppm, using cafestol acetate as reference compound for response factor determination as previously reported 43 …”

“…Fatty acid methyl esters were purified and detected using an Agilent 7890 gas chromatograph equipped with a DB-23 column (60 cm x 0.25 mm x 0.25 µm, Agilent Technologies, Wilmington, DE and flame ionization. Fatty acids were identified by comparing their retention time with commercial fatty acid standards (Sigma-Aldrich) and quantified using ChemStation (Agilent) to calculate the peak surfaces as previously described 43,44 . Volatile compounds were extracted using a CAR/PDMS (carboxen/polydimethylsiloxane, 75 µm) SPME fibre (Superlco Co., Bellefonter, PA, USA) and quantified by gas chromatography-mass spectroscopy (GC-MS) using a 6890A GC connected to a 5975B MS (Agilent, Palo Alto, USA) equipped with a 60 m ZB-WAX plus capillary column (film thickness: 0.25 µm; internal diameter: 0.25 mm; Phenomenex, Bologna, Italy).…”

Benchtop (60 MHz) proton NMR spectroscopy for quantification of 16-O-methylcafestol in lipophilic extracts of ground roast coffee

“… The spectrum of the 0 mg/ml sample also has a small peak at 3.16 ppm and a non-zero peak integral, consistent with the presence of 16-OMC at a low level. It is known that Arabica coffee beans can contain small but non-negligible quantities of this compound [8] , [9] , [10] , [11] , [12] . The 16-OMC analytical standard contains the freeform compound, whereas the native form in coffee is mostly esterified.…”

Physicochemical characteristics and bioactive compound profiles of Arabica Kalosi Enrekang with different postharvest processing

“…Among the beans, Arabica is deemed to be the most superior bean regarding organoleptic profiles, which make it more precious in global market. Arabica coffee brew is characterized with odoriferous and sweet-smelling, mild, and low acidity taste, as well as able to exert miscellaneous aroma (Portaluri et al, 2020). In this regard, South Sulawesi becomes the main producer of Arabica coffee in Indonesia.…”