Limited movement of Cucumber mosaic virus (CMV) in yellow passion flower in Brazil

Abstract

Symptoms of Cucumber mosaic virus (CMV) on yellow passion flower ( Passiflora edulis f. flavicarpa ) are characterized by bright yellow mottling on leaves, starting at random points on the vine and diminishing in intensity towards the tip, which becomes symptomless as it grows. To determine whether symptomless portions of vines are CMV-free or represent latent infection, leaves with and without symptoms were collected from infected vines in the field. Biological, serological (plate-trapped antigen enzyme-linked immunosorbent assay, PTA-ELISA), Western blot and dot-blot hybridization assays showed that portions of the vines without symptoms were CMV-free. Vegetatively propagated vines with symptoms showed remission of symptoms on newly developed leaves. One year later, no CMV was detected in the upper leaves of these plants. Mechanically inoculated passion flower seedlings behaved similarly; symptoms were shown by few leaves after inoculation. Afterwards, plants became symptomless and CMV was not detected in the upper leaves or root system, 40 or 85 days after inoculation. The mechanism responsible for remission of symptoms accompanied by CMV disappearance is not known.

Identification of a Cucumber mosaic virus Isolate from Passiflora edulis in Southern Italy and Validation of Subgroup Identification by In Silico Restriction Fragment Length Polymorphism

“…‘Woodiness’ is one of the most important viral diseases of passion fruit and can be elicited by two virus species, Passion fruit woodiness virus (PWV, genus Potyvirus ) and Cucumber mosaic virus (CMV, genus Cucumovirus ), which are often found in mixed infections (Singh, 2004). ‘Woodiness’ of P. edulis caused by CMV has been reported in several tropical countries where this species is cultivated, in some cases with a high incidence of the disease (Gioria et al., 2000, 2002). Nevertheless, the identification of CMV subgroup isolates affecting P. edulis cultivations has never been undertaken.…”

Incidence of Viral Diseases and Occurrence of Three Unreported Viruses in Yams in Korea

“…First-strand cDNA was (Gioria et al, 2002;Lee et al, 2004;Mumford and Seal, 1997;, and primers specific for two viruses (ChYNMV and JYMV) were designed based on published sequences from the National Center for Biotechnology Information (NCBI) ( Table 1). PCR conditions were adapted from previous reports, and conditions for detection of the two viruses with the newly developed primer sets were as follows: initial denaturation at 95°C for 10 min; 35 cycles at 95°C for 10 min, 55°C for 30 s, and 72°C for 1 min; and a final extension at 72°C for 5 min.…”

Caracterização da resistência da moranga (Cucurbita maxima) ´Exposição` ao Zucchini lethal chlorosis virus (ZLCV) e da não interferência de dois potyvirus na resistência das plantas

“…A resistência da moranga "Exposição" ao ZLCV foi pela primeira vez relatada por Giampan, Rezende e Silva (2007) A resistência da planta ao vírus expressa na forma de restrição ao movimento sistêmico de longa distância tem sido descrita para outros patossistemas (DUFOUR et al, 1989;GIORIA et al, 2002;MALLOR et al, 2003;MALLOR et al, 2006;SCHAAD;CARRINGTON, 1996). Resultado similar ao encontrado nesse trabalho foi observado por Goodrick, Kuhn e Hussey (1991) (NONO-WOMDIM et al, 1991).…”

“…Outra possibilidade seria ausência de algum tipo de sinal de reconhecimento do vírus, por exemplo, a capa protéica, envolvido no transporte vascular do patógeno, ou ainda uma falta de habilidade do vírus em modificar o tecido do hospedeiro para o transporte via plasmodesma. Gioria et al (2002) ao estudarem o movimento sistêmico limitado do CMV em hastes de maracujazeiro (Passiflora edulis f. flavicarpa) levantaram três hipóteses para tentar explicar o fenômeno: a primeira é a de que o vírus perde a habilidade de se replicar ou mover sistemicamente na planta; a segunda sugere um mecanismo de resistência da planta que é induzido com o progresso da infecção; e a terceira seria algum tipo de atividade inibitória na planta infectada que progressivamente prejudica a replicação do vírus e consequentemente a sua circulação na planta. Qualquer um desses mecanismos pode estar envolvido na resistência do movimento de longa distância do ZLCV em plantas de moranga "Exposição.…”