Limiting dilution analysis of proliferating and cytotoxic lymphocytes in the peripheral blood and tumours of oral cancer patients

Laad, Anupama D.; Kode, Jyoti; Chavan, Sangeeta S.; Rao, Renuka; Fakih, Abdul R.; Chiplunkar, Shubhada V.

doi:10.1016/0964-1955(96)00024-3

Cited by 14 publications

(7 citation statements)

References 17 publications

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“…By contrast, NK cells and antibody-dependent cellular cytotoxicity activities were within the normal range compared with healthy people in India where BQ chewing is popular 37 . Similar to LAAD et al 28 , the authors found an elevation of CD56+ and CD56+CD69+ NK cell populations in PBL during oral carcinogenesis. This implies that NK cell numbers and distribution gradually increased in response to tumour growth and metastasis during oral carcinogenesis possibly via adaptive immune responses.…”

Section: Effect Of Clinical Variables In Osccmentioning

confidence: 53%

Changes in peripheral blood lymphocyte phenotypes distribution in patients with oral cancer/oral leukoplakia in Taiwan

Lee¹,

Lin²,

Chen³

et al. 2010

International Journal of Oral and Maxillofacial Surgery

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Section: Effect Of Clinical Variables In Osccmentioning

confidence: 53%

Changes in peripheral blood lymphocyte phenotypes distribution in patients with oral cancer/oral leukoplakia in Taiwan

Lee¹,

Lin²,

Chen³

et al. 2010

International Journal of Oral and Maxillofacial Surgery

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“…There is substantial evidence indicating that immune responses, which should otherwise suppress or eliminate oral cancer cells, are inhibited by properties and functions of oral cancers. NK cells and cytotoxic T-cells, which play crucial effector functions in the host defense against neoplasia, are functionaly inactivated in oral cancers (Trinchieri, 1989;Jewett et al, , 1997Jewett et al, , 2003Jewett et al, , 2006aLaad et al, 1996). Regressing tumor grafts of oral origin contain significantly larger numbers of functional NK and T-cells than those associated with the primary tumors (Thomas et al, 1995), while patients with metastasis of head and neck cancers have low NK and T-cell activity (Schantz et al, 1986).…”

Section: Immune Responses In Head and Neck Cancersmentioning

confidence: 99%

Emerging Mechanisms of Immunosuppression in Oral Cancers

2006

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Mounting effective anti-tumor immune responses against tumors by both the innate and adaptive immune effectors is important for the clearance of tumors. However, accumulated evidence indicates that immune responses that should otherwise suppress or eliminate transformed cells are themselves suppressed by the function of tumor cells in a variety of cancer patients, including those with oral cancers. Signaling abnormalities, spontaneous apoptosis, and reduced proliferation and function of circulating natural killer cells (NK), T-cells, dendritic cells (DC), and tumor-infiltrating lymphocytes (TILs) have been documented previously in oral cancer patients. Several mechanisms have been proposed for the functional deficiencies of tumor-associated immune cells in oral cancer patients. Both soluble factors and contact-mediated immunosuppression by the tumor cells have been implicated in the inhibition of immune cell function and the progression of tumors. More recently, elevated levels and function of key transcription factors in tumor cells, particularly NFkappaB and STAT3, have been shown to mediate immune suppression in the tumor microenvironment. This review will focus on these emerging mechanisms of immunosuppression in oral cancers.

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“…(13) In addition, the proliferative response and cytotoxic activity of lymphocytes were attenuated in oral cancer patients. (14) Likewise, lymphocytes isolated from regional lymph nodes in head and neck cancer patients showed a diminished response of T-cell proliferation. (15) On the basis of these clinical studies showing a potential association between immune deregulation and the pathophysiology of areca-related oral diseases, it is speculated that AQ ingredients may possess immunomodulatory and proinflammatory activities.…”

mentioning

confidence: 99%

Areca-nut extract modulates antigen-specific immunity and augments inflammation in ovalbumin-sensitized mice

Lin

Wey

Jan

2010

Immunopharmacology and Immunotoxicology

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Areca-nut chewing has been linked to oral cancer and many other diseases, in which immune deterioration and tissue inflammation are plausibly involved. Recent studies reported that areca-nut extract (ANE) affected the functionality of lymphocytes and neutrophils in vitro. In the present study, we investigated the immunomodulatory effect of ANE in vivo. Ovalbumin (OVA)-sensitized mice were daily administered with ANE (5-50 mg/kg) for 10 doses by intraperitoneal injection from days 1 to 5 and from 8 to 12. The mice were systemically sensitized with OVA on day 3, and their footpads were challenged with OVA to induce delayed-type hypersensitivity (DTH) reactions on day 13. The serum level of OVA-specific IgM and IgG(1) was significantly attenuated by 5 and 25 mg/kg of ANE, whereas OVA-specific IgG(2a) was markedly enhanced by 50 mg/kg of ANE. The production of interferon (IFN)-γ by splenocytes reexposed to OVA in culture was markedly augmented by ANE (25 and 50 mg/kg). In addition, ANE (25 and 50 mg/kg) demonstrated an enhancing effect on DTH reactions, including the tissue swelling, the infiltration of CD3(+) and F4/80(+) cells, and the expression of IFN-γ and tumor necrosis factor (TNF)-α in the footpads challenged with OVA. The phagocytic activity and TNF-α production by the splenic CD11b(+) cells were also enhanced in ANE-treated groups. Taken together, these results demonstrated that ANE modulated antigen-specific immune responses and promoted inflammatory reactions in vivo, which may contribute to immune deregulation associated with areca-related diseases.

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Limiting dilution analysis of proliferating and cytotoxic lymphocytes in the peripheral blood and tumours of oral cancer patients

Cited by 14 publications

References 17 publications

Changes in peripheral blood lymphocyte phenotypes distribution in patients with oral cancer/oral leukoplakia in Taiwan

Changes in peripheral blood lymphocyte phenotypes distribution in patients with oral cancer/oral leukoplakia in Taiwan

Emerging Mechanisms of Immunosuppression in Oral Cancers

Areca-nut extract modulates antigen-specific immunity and augments inflammation in ovalbumin-sensitized mice

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