Bioconversion
of renewable H
2
and waste CO
2
using methanogenic
archaea is a promising technology for obtaining
high-purity CH
4
, which can serve as an alternative for
natural gas. This process is known as ex situ biogas upgrading. This
work highlights the pathway toward the bioconversion of renewable
H
2
and CO
2
into high-purity biomethane by exploiting
highly accessible agro-municipal residues: cow manure (CM) and the
organic fraction of solid municipal waste (OFSMW), which used to be
called “waste materials”. More specifically, an ex situ
thermophilic (55 °C) biogas upgrading process was conducted by
CM and OFSMW codigestion at different mass proportions: 100:0, 80:20,
70:30, 60:40, and 50:50. Maximum CH
4
concentrations of
92–97 vol % and biogas volumetric production rates of 4954–6605
NmL/L.d were obtained from a batch reactor of 3 L working volume.
Feedstock characterization, pH monitoring, and the carbon-to-nitrogen
ratio were critical parameters to evaluate during biogas upgrading
experiments. In this work, the usefulness of agro-municipal substrates
is highlighted by producing high-purity biomethane—an energetic
chemical to facilitate renewable energy conversion, which supports
various end-use applications. This process therefore provides a solution
to renewable energy storage challenges and future sustainable and
green energy supply.