Limits of rickettsial infectivity

Ormsbee, Richard A.; Peacock, Marius G.; Gerloff, Robert K.; Tallent, George; Wike, David A.

doi:10.1128/iai.19.1.239-245.1978

Cited by 86 publications

(40 citation statements)

References 21 publications

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“…The results obtained for milk samples from 21 cows proved that the system could detect coxiellae in naturally infected specimens. This is important as C. burnetii is a strictly intracellular agent (16). Findings obtained with artificially contaminated samples containing only extracellular C. burnetii particles cannot simply be transferred to clinical samples.…”

Section: Discussionmentioning

confidence: 99%

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PCR Detection ofCoxiella burnetiifrom Different Clinical Specimens, Especially Bovine Milk, on the Basis of DNA Preparation with a Silica Matrix

Lorenz

Jäger

Willems

et al. 1998

Appl Environ Microbiol

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For PCR detection of Coxiella burnetii in various clinical specimens we developed a sample preparation method in which silica binding of DNA was used. This method was found to be fast, easily performed with large numbers of samples, and equally sensitive for all of the specimens tested (livers, spleens, placentas, heart valves, milk, blood). The DNA preparation method described here can also be used as an initial step in any PCR-based examination of specimens. The procedure was tested with more than 600 milk samples, which were taken from 21 cows that were seropositive for C. burnetii and reportedly had fertility problems (and therefore were suspected of shedding the agent through milk intermittently or continuously). Of the 21 cows tested, 6 were shedding C. burnetii through milk. Altogether, C. burnetii DNA was detected in 6% of the samples. There was no correlation between the shedding pattern and the serological results.

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Section: Discussionmentioning

confidence: 99%

“…Whereas animals in general show no clinical signs of infection except occasional abortions and other problems with reproduction, C. burnetii can cause serious illness in humans. This agent is very resistant to environmental influences, and even a single infective particle can initiate an infection in the animal model (16).…”

mentioning

confidence: 99%

PCR Detection ofCoxiella burnetiifrom Different Clinical Specimens, Especially Bovine Milk, on the Basis of DNA Preparation with a Silica Matrix

Lorenz

Jäger

Willems

et al. 1998

Appl Environ Microbiol

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“…Embryonated chicken egg yolk sacs have been widely used in the past (39), but they are now being replaced by cell culture systems. Inoculation into guinea pigs has also been widely used (66,103). The mouse is the species of choice for the isolation of R. akari, Rickettsia australis, and especially O. tsutsugamushi.…”

Section: Isolation Of Rickettsiaementioning

confidence: 99%

Laboratory diagnosis of rickettsioses: current approaches to diagnosis of old and new rickettsial diseases

1997

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“…By way of comparison, earlier studies in Hartley guinea pigs (Ormsbee et al, 1978) showed that only two viable C. burnetii (phase 1) cells would produce a fever, a similar sensitivity to the SCID mouse spleen assay.…”

Section: Discussionmentioning

confidence: 97%

Detecting and measuring small numbers of viableCoxiella burnetii

Lockhart¹,

Islam

Graves

et al. 2012

FEMS Immunol Med Microbiol

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Coxiella burnetii is an acidophilic, intracellular bacterium that causes the human disease Q fever. In some studies, it is important to distinguish between viable and nonviable C. burnetii. We compared four methods for detecting and measuring viable C. burnetii in biological samples as follows: growth in two different cell culture lines, infection of severe combined immunodeficient (SCID) mice (leading to death) and infection of SCID mice with detection of C. burnetii in their spleen (after euthanasia at day 50 postinfection). Two isolates of C. burnetii were used ('Henzerling' and 'Arandale'). Our in-house qPCR assay for C. burnetii DNA was used as a control. SCID mouse inoculation was more sensitive than cell culture. The assay that detected C. burnetii in SCID mouse spleens was slightly more sensitive than SCID mice deaths alone. Approximately one viable C. burnetii cell could be detected by this method, making it suitable for determining the viability of C. burnetii in a sample.

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Limits of rickettsial infectivity

Cited by 86 publications

References 21 publications

PCR Detection ofCoxiella burnetiifrom Different Clinical Specimens, Especially Bovine Milk, on the Basis of DNA Preparation with a Silica Matrix

PCR Detection ofCoxiella burnetiifrom Different Clinical Specimens, Especially Bovine Milk, on the Basis of DNA Preparation with a Silica Matrix

Laboratory diagnosis of rickettsioses: current approaches to diagnosis of old and new rickettsial diseases

Detecting and measuring small numbers of viableCoxiella burnetii

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