Limosilactobacillus (Lactobacillus) fermentum ALAL020, a Probiotic Candidate Bacterium, Produces a Cyclic Dipeptide That Suppresses the Periodontal Pathogens Porphyromonas gingivalis and Prevotella intermedia

Kawai, Tomomi; Ohshima, Tomoko; Tanaka, Takeshi; Ikawa, Satoshi; Tani, Atsushi; Inazumi, Naoya; Shin, Ryoichi; Itoh, Yukie; Meyer, Karen; Maeda, Nobuyo

doi:10.3389/fcimb.2022.804334

Cited by 8 publications

(6 citation statements)

References 37 publications

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“…In addition, some strains of bacteria isolated from the oral cavity have been produced commercially as probiotics, including Lactobacillus reuteri , Lactobacillus brevis and Streptococcus salivarius [ 25 , 43 ]. Kawai et al [ 44 ] suggested that Limosilactobacillus (Lactobacillus) fermentum ALAL020 may be a future probiotic candidate. This bacterium produces a cyclic dipeptide with antibacterial activity against Porphyromonas gingivalis and Prevotella intermedia [ 44 ].…”

Section: Microbiome In the Oral Healthmentioning

confidence: 99%

“…Kawai et al [ 44 ] suggested that Limosilactobacillus (Lactobacillus) fermentum ALAL020 may be a future probiotic candidate. This bacterium produces a cyclic dipeptide with antibacterial activity against Porphyromonas gingivalis and Prevotella intermedia [ 44 ]. Currently, the beneficial effects of synbiotics on health are of great interest to scientific research.…”

Section: Microbiome In the Oral Healthmentioning

confidence: 99%

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The Role of the Oral Microbiome in the Development of Diseases

Kozak

Pawlik

2023

IJMS

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Periodontal disease (PD) is a complex and infectious illness that begins with a disruption of bacterial homeostasis. This disease induces a host inflammatory response, leading to damage of the soft and connective tooth-supporting tissues. Moreover, in advanced cases, it can contribute to tooth loss. The aetiological factors of PDs have been widely researched, but the pathogenesis of PD has still not been totally clarified. There are a number of factors that have an effect on the aetiology and pathogenesis of PD. It is purported that microbiological, genetic susceptibility and lifestyle can determine the development and severity of the disease. The human body’s defence response to the accumulation of plaque and its enzymes is known to be a major factor for PD. The oral cavity is colonised by a characteristic and complex microbiota that grows as diverse biofilms on all mucosal and dental surfaces. The aim of this review was to provide the latest updates in the literature regarding still-existing problems with PD and to highlight the role of the oral microbiome in periodontal health and disease. Better awareness and knowledge of the causes of dysbiosis, environmental risk factors and periodontal therapy can reduce the growing worldwide prevalence of PDs. The promotion of good oral hygiene, limiting smoking, alcohol consumption and exposure to stress and comprehensive treatment to decrease the pathogenicity of oral biofilm can help reduce PD as well as other diseases. Evidence linking disorders of the oral microbiome to various systemic diseases has increased the understanding of the importance of the oral microbiome in regulating many processes in the human body and, thus, its impact on the development of many diseases.

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Section: Microbiome In the Oral Healthmentioning

confidence: 99%

Section: Microbiome In the Oral Healthmentioning

confidence: 99%

The Role of the Oral Microbiome in the Development of Diseases

Kozak

Pawlik

2023

IJMS

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“…38 In addition, a cyclic dipeptide synthesized by Lactobacillus fermentum ALAL020 was found to have a significant inhibitory effect on the growth and activity of P. gingivalis and Prevotella intermedia (P. intermedia). 39 Lactobacillus helveticus can enrich the expression of β-defensins in gingival epithelial cells, thereby inhibiting the inflammatory response induced by P. gingivalis. 40 The bipeptide bacteriocin PLNC8 αβ produced by Lactobacillus plantarum can also significantly inhibit P. gingivalis.…”

Section: Discussionmentioning

confidence: 99%

Efficacy of photodynamic therapy as an adjunct to scaling and root planing on clinical parameters and microbial composition in subgingival plaque of periodontitis patients: A split‐mouth randomized clinical trial

Nie,

Huang,

Peng

et al. 2024

Journal of Periodontology

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BackgroundThe aim of this study was to assess the efficacy of photodynamic therapy (PDT) as an adjunct to scaling and root planing (SRP) on clinical parameters and microbial composition in subgingival plaque of periodontitis patients.MethodsSeventeen patients were included in this split‐mouth randomized clinical trial. Sites with probing pocket depth (PPD) ≥5 mm in combination with bleeding on probing in different quadrants were randomized into the control group, the group with a single PDT application right after SRP, and the group with three repeated PDT applications 1 week after SRP. The subgingival plaque was collected for 16S rRNA gene sequencing at baseline, Week 2, and Week 8.ResultsSeventeen patients with 60 sites completed this 8‐week follow‐up, and 157 subgingival plaques were successfully analyzed by sequencing. Significant improvements were observed in two primary outcomes: PPD at Week 8 and subgingival microbial composition. Compared to the control group, the repeated‐PDT group showed a notable improvement in PPD, substantial alterations in the microbial profile, including a reduction in α‐diversity and anaerobic bacteria, and an increase in aerobic bacteria at Week 2. Secondary outcomes, such as clinical attachment level and sulcus bleeding index, also showed improvement at Week 8. Furthermore, both the single‐ and repeated‐PDT groups exhibited a decrease in periodontopathogens and an increase in beneficial bacteria compared with baseline.ConclusionPDT promotes changes in the microbial composition of periodontitis patients’ subgingival plaque in a direction favorable to periodontal health, and repeated PDT is a promising adjunctive therapy for periodontal treatment.

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“…Nisin has also been found to be effective against A. actinomycetemcomitans, P. intermedia, P. gingivalis, F. nucleatum, and Treponema denticola 40 . Other bacteriocin-like peptides have also been found to be effective against periodontal pathobionts 41,42 .…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial potential of known and novel probiotics on in vitro periodontitis biofilms

Teughels

Holm

Carvalho

et al. 2022

Preprint

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Several oral diseases are characterized by a shift within the oral microbiome towards a pathogenic, dysbiotic composition. Broad spectrum antimicrobials are often part of their treatment. However, with the rising antibiotic resistance, alternatives are increasingly desirable. Alternatively, supplying beneficial species through probiotics is increasingly showing beneficial results, however, these probiotics are rarely evaluated comparatively. In this study, the in vitro effects of three known- and three novel lactobacillus strains, together with four novel Streptococcus salivarius strains were comparatively evaluated for antagonistic effects on proximal agar growth, antimicrobial properties of probiotic supernatant and the probiotic's effects on in vitro periodontal biofilms. Strain specific effects were observed as differences between genera and differences in efficacy within genera. While some of the lactobacillus candidates were able to reduce the periodontal pathobiont A. actinomycetemcomitans, the S. salivarius strains were not. However, the S. salivarius strains were more effective against P. intermedia, P. gingivalis and F. nucleatum. Most of the lactobacillus strains also negatively affected the prevalence commensal species within the biofilms, while this was lower for S. salivarius strains. Both within lactobacilli and streptococci some strains showed significantly more inhibition of the pathobionts, indicating the importance of proper strain selection. Additionally, some species showed reductions in non-target species, which can result in unexpected and unexplored effects on the whole microbiome.

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Limosilactobacillus (Lactobacillus) fermentum ALAL020, a Probiotic Candidate Bacterium, Produces a Cyclic Dipeptide That Suppresses the Periodontal Pathogens Porphyromonas gingivalis and Prevotella intermedia

Cited by 8 publications

References 37 publications

The Role of the Oral Microbiome in the Development of Diseases

The Role of the Oral Microbiome in the Development of Diseases

Efficacy of photodynamic therapy as an adjunct to scaling and root planing on clinical parameters and microbial composition in subgingival plaque of periodontitis patients: A split‐mouth randomized clinical trial

Antimicrobial potential of known and novel probiotics on in vitro periodontitis biofilms

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