Limosilactobacillus reuteri inhibits the acid tolerance response in oral bacteria

Boisen, Gabriella; Prgomet, Zdenka; Enggren, Gabriela; Dahl, Hanna; Mkadmi, Cindy; Davies, Julia R.

doi:10.1016/j.bioflm.2023.100136

Cited by 6 publications

(3 citation statements)

References 47 publications

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“…Parallel session 3, “Heterogeneity and metabolic adaptations at the special biofilm microenvironment” was chaired by Thomas Bjarnsholt (Costerton Biofilm Center, Copenhagen, Denmark) and Susanne Häussler, and this special issue includes several papers related to this session, including that of Cornejo et al [ 6 ], which focuses on the effect of sex steroid hormones on the ecology of in vitro oral biofilms. Similarly, the research presented by Davis et al [ 7 ] shows that plaque acid-tolerance was increased in individuals with active caries compared to caries-free individuals. In addition to specific findings, the study proves the possible role of the probiotic strain Limosilactobacillus reuteri in caries prevention by inhibiting the development of an acid-tolerant biofilm microbiota.…”

mentioning

confidence: 60%

Eurobiofilms 2022: A translational perspective of biofilm-related persistent infections

Macià,

Borghi,

Oliver

2024

Biofilm

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confidence: 60%

Eurobiofilms 2022: A translational perspective of biofilm-related persistent infections

Macià,

Borghi,

Oliver

2024

Biofilm

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“…Lately, there has been a growing interest in enhancing a healthy oral microbiome and preventing diseases by utilizing probiotic bacteria. 15 In general, several lactobacilli strains have been proposed as adjuncts to "good clinical practice" for managing various oral health issues such as childhood caries, gingivitis, periodontal disease, candidiasis, and halitosis. Furthermore, these beneficial bacteria might have the potential in enhancing the healing process of surgical wounds by influencing the immune response systemically and through the up-regulation of the neuropeptide hormone oxytocin.…”

Section: Discussionmentioning

confidence: 99%

Limosilactobacillus reuteri DSM 17938 Inhibition of Biofilm Formation by Prevotella intermedia and Fusobacterium nucleatum Across Salivary pH: An In Vitro Study

Ananda,

Julia,

Bachtiar

2024

Eur J Dent

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Objectives This study aims to investigate Limosilactobacillus reuteri DSM 17938's antibiofilm effects on Prevotella intermedia and Fusobacterium nucleatum, common causes of alveolar osteitis. It seeks topical alternatives to prevent this condition posttooth extraction. The secondary objective is to assess these effects under different pH conditions (pH 4.5 and pH 7), mimicking oral cavity saliva pH dynamics. Materials and Methods Ethical approval was secured for the saliva collection process involving five healthy adult participants who had undergone wisdom tooth extraction. Saliva samples were diligently collected on the 7th day post-surgery. The unstimulated saliva underwent a series of treatments, including the addition of phenylmethylsulfonyl fluoride (PMSF), pH adjustments, centrifugation, and filtration. The pH levels were re-measured, and subsequent adjustments were made to achieve pH values of 4.5 or 7. Limosilactobacillus reuteri DSM 17938, with a concentration of 1×108 colony-forming units (CFU) per 5 drops, was utilized in the study. Biofilm testing involved incubating saliva samples with varying pH (4.5 or 7) alongside bacterial suspensions (Prevotella intermedia, Fusobacterium nucleatum, or a mixed species). The Interlac suspension was introduced, and plates were anaerobically incubated for 24 hours. Biofilm results were obtained using a spectrometer. The test is conducted in triplicate. Statistical Analysis To scrutinize the impact of pH on biofilm development, the acquired data underwent a two-way ANOVA test in SPSS as part of the statistical analysis. A significance level of p<0.05 was used to determine statistical significance. Results Limosilactobacillus reuteri DSM 17938 significantly reduced biofilm formation across bacterial strains (p = 0.000). Statistical analysis indicated a significant impact of pH on biofilm development (p = 0.000) compared to no saliva samples, with higher formation observed under acidic conditions (pH 4.5). However, the pH levels of 4.5 and 7 did not result in significantly different bacterial biofilm formation (p = 0.529). Conclusion This research highlights Limosilactobacillus reuteri DSM 17938's potency in inhibiting biofilm formation of Prevotella intermedia and Fusobacterium nucleatum. Salivary pH variations significantly influence biofilm development, emphasizing the need to consider pH when assessing probiotic effectiveness. Despite limitations in saliva sample sterilization, this study provides valuable insights into alternative approaches for preventing alveolar osteitis. Further research should explore clinical applications and refine sterilization methods for more accurate results.

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“…The undamaged cells continue giving off a green signal, and in this way the viability of bacterial cells as a function of membrane integrity can be monitored. To verify that cells that give off red signals are dead or dying and cannot repair and regrow, a previous study in our department compared the ratios of Live/Dead cells from the viability assay of another Gram positive oral bacteria (Limosilactobacillus reuteri) with culture of samples on agar and calculation of CFU (96). The ratio was found to be consistent, and the BacLight TM LIVE/DEAD® was thereby shown to be a reliable tool for assessing the ratio of viable bacterial cells in a population.…”

Section: Biofilm Viability Assaymentioning

confidence: 99%

Responses to External Cues in Oral Bacteria

Robertsson

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This thesis investigates responses to external cues in oral bacteria on a molecular level. Paper I maps Ser/Thr/Tyr phosphorylated proteins in relation to the general proteome in an oral commensal streptococcus (Streptococcus gordonii DL1). The identified phosphoproteins were involved in various bacterial processes, several associated to dysbiosis and development of biofilm-induced disease. Comparison against phosphoproteomes of other bacteria showed many similarities. This is of interest for the identification of shared phosphorylation profiles. Paper II studies differences between the S. gordonii DL1 general proteomes in planktonic and biofilm growth phases, and the regulatory effects of salivary mucin MUC5B on protein expression in the biofilm cells. Regulations in protein expression between the different growth conditions provides insights in bacterial mechanisms for adaptation to the biofilm lifestyle. Paper III examines the regulatory roles of salivary MUC5B on biofilm attachment and metabolic output in two clinical isolates of oral commensals, S. gordonii CW and Actinomyces naeslundii CW. S. gordonii facilitated adhesion of A. naeslundii to MUC5B during early attachment. Both bacteria were also able to utilize MUC5B as a sole nutrient source during early biofilm formation, individually and synergistically in a dual species biofilm. The specific responses elicited by MUC5B in paper II-III seem to promote commensal colonization while down-regulating dysbiosis-related biofilm activities. Microbiological studies are often focused on dysbiosis and development of disease. However, mechanisms that promote eubiosis are equally important to understand how health can be maintained. Findings associated with responses to external cues in oral bacteria may contribute to future development of novel preventative strategies and identification of predictive biomarkers for oral health.

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Limosilactobacillus reuteri inhibits the acid tolerance response in oral bacteria

Cited by 6 publications

References 47 publications

Eurobiofilms 2022: A translational perspective of biofilm-related persistent infections

Eurobiofilms 2022: A translational perspective of biofilm-related persistent infections

Limosilactobacillus reuteri DSM 17938 Inhibition of Biofilm Formation by Prevotella intermedia and Fusobacterium nucleatum Across Salivary pH: An In Vitro Study

Responses to External Cues in Oral Bacteria

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