Linalool loaded on glutathione-modified gold nanoparticles: a drug delivery system for a successful antimicrobial therapy

Abstract

In the present study, antimicrobial activity of Linalool loaded on Glutathione-modified Gold nanoparticles prepared by novel method was investigated. The aim of this study is to evaluate the antimicrobial activity of Linalool-gold nanoparticles (LIN-GNPs) against Gram's positive bacteria Staphylococcus aureus, Gram's negative bacteria Escherichia coli, and against Leishmania tropica. Gold nanoparticles were synthesized using the chemical method. Colour change, UV-Vis spectrum, FTIR and SEM confirmed the characterization of gold nanoparticles and LIN-GNPs. The antibacterial study was including agar well diffusion method, MIC, MBC. The mode of action was determined by cellular material release assay, SEM and AO/EtBr for ROS detection. Anti-parasitic activity was evaluated using MTT assay. FTIR spectral analysis investigated that Linalool was loaded on gold nanoparticles. SEM showed that the Gold nanoparticles and LIN-GNPs were generally found to be spherical in shape and the size was ranged 5-11 nm for GNPs and 15-20 nm for LIN-GNPs. The results of antibacterial activity demonstrated that Linalool alone had low activity against gram-positive and gram-negative bacteria. While the results showed that gram-positive bacteria were more effective by LIN-GNPs. LIN-GNPs acted on the bacterial cell membrane, resulting in loss of integrity and increased permeability of cell wall and stimulated ROS production that leads to damage of bacterial nucleic acid. The anti-parasitic activity results indicated the high activity of LIN-GNPs on L. tropica compared with Linalool and Gold nanoparticles. These results proved that LIN-GNPs have great potential as antimicrobial activity and could be used as a developing strategy for a successful antimicrobial therapeutic agent.

Zinc Oxide Nanoparticles Induces Apoptosis in Human Breast Cancer Cells via Caspase-8 and P53 Pathway

“…Acridine orange was taken up via viable cells and emitted green fluorescence. Ethidium bromide was taken up only via nonviable cells and emitted red fluorescence through intercalation with harm DNA [15]. Cells line was seeded with density (1×10 4 ) on the cover slide that was located at the 12-well plate with ZnO nanoparticles.…”

Zinc Oxide Nanoparticles and Nanorods as Antimicrobial Agents: Particle Size Influence

“…The findings have confirmed that the small size of nanoparticles is supporting the ease of entering microbial cell membrane and thereby allowing inhibition mechanisms to activate within the cell [26]. It may also be explained by the involvement of reactive oxygen species (ROS) production [27,28], and accumulation of nanoparticles within the cytoplasm or outer membranes [29,30], disruption of the cell membrane and oxidative stress [31]. Findings illustrate that Gram-positive bacteria (Strept.…”

Polyethylene Glycol-Functionalized Magnetic (Fe3O4) Nanoparticles: A Novel DNA-Mediated Antibacterial Agent

“…This method related optical density (OD) of the culture media at 220 nm to the time. As shown in against bacterial strains such as Staphylococcus and Escherichia coli [24]. Another study showed the ability of carbon NPs decorated with cupric oxide in reduction of bacterial growth [25].…”