Linamarase activities in Bacillus spp. responsible for thermophilic aerobic digestion of agricultural wastes for animal nutrition

Ugwuanyi, J. Obeta; Harvey, Linda M.; McNeil, Brian

doi:10.1016/j.wasman.2006.10.020

Cited by 8 publications

(1 citation statement)

References 31 publications

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“…whereas Neurospora sitophila and Geotrichum candidum hardly degraded the linamarin. Ugwuanyi et al (2007) described Bacillus species including Bacillus coagulans, Bacillus licheniformis and Bacillus stearothermophilus, able to degrade linamarin. The use of HPLC for the quantification of the microbial linamarin degradation and other metabolites (HCN and CO 2 ) was an innovation introduced in the present work.…”

Section: Hplc Analysis Of the Products Of Degradation Of Linamarin Bymentioning

confidence: 99%

In vitro Degradation of Extracted Cassava Linamarin by Bacillus Species Isolated from cassava wastewater

Ogunyemi

Samuel

Buraimoh

et al. 2017

Egyptian Academic Journal of Biological Sciences, G. Microbiolo

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Linamarin is the most abundant cyanoglucoside present in cassava cells and may generate the equivalent amount of hydrocyanic acid. This study was aimed to assess degradative capacities of Bacillus pumilus strain WOB3 and WOB7on linamarin. The test organisms for linamarase activity were identified on the basis of phenotype, biochemical properties and 16S rDNA gene sequencing as: Bacillus pumilus strain WOB3 KX774195 and Bacillus pumilus strain WOB7 KX774196. Growth studies showed that the strains grew in all the substrates tested. The doubling times of Bacillus pumilus strain WOB3 and Bacillus pumilus strain WOB7 were 8.25 d and 7.53 d on cassava effluent, 6.30 d and 5.78 d on supplemented cassava effluent, 8.66 d and 9.90 d on waste leachate and 6.30 d and 9.24 d on supplemented waste leachate respectively; with specific growth rates of 0.084 d-1 and 0.092 d-1 on cassava effluent, 0.11 d-1 and 0.12 d-1 on supplemented cassava effluent, 0.080 d-1 and 0.070 d-1 on waste leachate and 0.11 d-1 and 0.075 d-1 on supplemented waste leachate respectively. High-performance liquid chromatographic analysis studies revealed that linamarin degradation by the strains followed a linamarase pathway involving CO 2 and HCN as metabolic intermediates. Based on HPLC analysis, linamarin residual concentration at day 12 by the strains WOB3 and WOB7 was 26.73 mgL-1 (19.79 %) and 29.79 mgL-1 (21.92 %). These novel features make the bacteria suitable candidates for in-situ application on sites contaminated with cassava processing wastes.

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Section: Hplc Analysis Of the Products Of Degradation Of Linamarin Bymentioning

confidence: 99%