LINC00152 knock-down suppresses esophageal cancer by EGFR signaling pathway

Abstract

<jats:title>Abstract</jats:title><jats:sec id="j_med-2020-0019_s_005_w2aab3b7d134b1b6b1aab1c16b1Aa"><jats:title>Aim</jats:title><jats:p>This study aims to explain the role and mechanism of lncRNA LINC00152 in esophageal cancer.</jats:p></jats:sec><jats:sec id="j_med-2020-0019_s_006_w2aab3b7d134b1b6b1aab1c16b2Aa"><jats:title>Methods</jats:title><jats:p>The 30 pairs of esophageal cancer and adjacent normal tissues were collected and measuring the lncRNA LINC00152 expression by ISH and RT-qPCR assay. In the next cell experiment, Eca 109 and Kyse 150 cells were divided into 3 groups: NC group were treated with non-treatment; BL group were transfected with empty vector and lncRNA group were transfected with lncRNA LINC00152. The cells proliferation were measured by MTT assay; the cells apoptosis and cell cycle were evaluated by flow cytometry. The relative proteins expressions were measured by WB assay.</jats:p></jats:sec><jats:sec id="j_med-2020-0019_s_007_w2aab3b7d134b1b6b1aab1c16b3Aa"><jats:title>Results</jats:title><jats:p>Compared with NC groups, the cell proliferation rate of lncRNA groups were significantly suppressed (P&lt;0.05, respectively); the cell apoptosis and G1 phase rates were significantly enhanced in the lncRNA groups (P&lt;0.05, respectively). In the proteins expressions, the EGFR, PI3K and AKT proteins expressions of lncRNA group were significantly inhibited and the P21 proteins expressions were significantly stimulated in the lncRNA groups compared with those of NC groups in Eca 109 and Kyse 150 cells.</jats:p></jats:sec><jats:sec id="j_med-2020-0019_s_008_w2aab3b7d134b1b6b1aab1c16b4Aa"><jats:title>Conclusion</jats:title><jats:p>The lncRNA LINC00152 had anti-tumor effects on esophageal cancer in the Eca 109 and Kyse 150 cells, the mechanisms were relative with EGFR pathway.</jats:p></jats:sec>

Nobiletin in Cancer Therapy: How This Plant Derived-Natural Compound Targets Various Oncogene and Onco-Suppressor Pathways

“…β-elemene as an antitumor agent diminishes the migration and invasion of cancer cells by the inhibition of EGFR signaling [224]. The onco-suppressor upstream factors are able to inhibit cancer malignancy via the inhibition of EGFR [225]. These studies exhibit that EGFR signaling is a positive factor for the growth and proliferation of cancer cells, and its targeting is a potential strategy in cancer therapy.…”

<p>Overexpression of Rab40b Promotes Hepatocellular Carcinoma Cell Proliferation and Metastasis via PI3K/AKT Signaling Pathway</p>

“…27,28 Conversely, p21 is generally considered a negative regulator of G1/S transition. 25,26 Therefore, we determined the expression level of these cell cycle-related proteins by Western blotting. Our results indicated that the downregulation of submit your manuscript | www.dovepress.com…”

“… 27 , 28 Conversely, p21 is generally considered a negative regulator of G1/S transition. 25 , 26 Therefore, we determined the expression level of these cell cycle-related proteins by Western blotting. Our results indicated that the downregulation of cyclin D1 and cyclin E1 and the upregulation of p21 may induce G0/G1 cell cycle arrest in HCC cells after Rab40b knockdown.…”

Long non-coding RNA LINC00152 in cancer: Roles, mechanisms, and chemotherapy and radiotherapy resistance

“…Functionally, the overexpression of LINC00152 promotes the proliferation, invasion, and migration of ESCC cells in vitro and also regulates the interaction between mitotic arrest-deficient 2-like 1 (MAD2L1) and cyclin-dependent kinase 6 (CDK6) in vesicle transport pathway proteins, and syntaxin 3 (STX3) and STX12 soluble N-ethylmaleimide-sensitive factor-attachment protein (SNAP) receptor (SNARE) family members ( 64 ). Ding et al ( 63 ) found that LINC00152 knockdown might inhibit proliferation and induce apoptosis of Eca-109 and KYSE-150 cells by inhibiting the anti-tumor epidermal growth factor receptor EGFR/PI3K/AKT pathway and enhancing P21 expression in EC ( 63 ). In addition, Zhou et al ( 38 ) found that LINC00152 regulates Rab10 by sponging miR-107 to promote cell proliferation, migration, and invasion in ESCC ( 65 ).…”