LINC00210 plays oncogenic roles in non‑small cell lung cancer by sponging microRNA‑328‑5p

Liu, Zhengjia; Xu, Lei; Zhang, Kejian; Guo, Bo; Cui, Zhi; Gao, Nan

doi:10.3892/etm.2020.8593

Cited by 3 publications

(9 citation statements)

References 31 publications

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“…Results revealed that LINC00210 was up-regulated in osteosarcoma tissues and cells, which was consistent with previous reports. 17 The expression of LINC00210 was increased in NSCLC, 16 liver cancer, 17 and thyroid cancer. 18 Besides, the expression of LINC00210 was further increased in osteosarcoma cells with irradiation treatment, which indicated that LINC00210 might involve in the modulation of osteosarcoma radioresistance.…”

Section: Discussionmentioning

confidence: 99%

“…16,17 For example, LINC00210 presented aberrant expression in liver cancer, thyroid cancer, and non-small cell lung cancer (NSCLC). [16][17][18] LINC00210 could promote NSCLC progression by functioning as the sponge of miR-328-5p. 16 Besides, LINC00210 also increased the malignancy of thyroid cancer via regulating miR-195-5p/IGF1R.…”

Section: Introductionmentioning

confidence: 99%

“…For example, lncRNA TTN‐AS1 modulated osteosarcoma cell drug resistance through the miR‐134‐5p/MBTD1 axis 15 . LncRNA LINC00210 is a vital lncRNA and plays an essential role in many cancers 16,17 . For example, LINC00210 presented aberrant expression in liver cancer, thyroid cancer, and non‐small cell lung cancer (NSCLC) 16‐18 .…”

Section: Introductionmentioning

confidence: 99%

“…15 LncRNA LINC00210 is a vital lncRNA and plays an essential role in many cancers. 16,17 For example, LINC00210 presented aberrant expression in liver cancer, thyroid cancer, and non-small cell lung cancer (NSCLC). [16][17][18] LINC00210 could promote NSCLC progression by functioning as the sponge of miR-328-5p.…”

Section: Introductionmentioning

confidence: 99%

“…[16][17][18] LINC00210 could promote NSCLC progression by functioning as the sponge of miR-328-5p. 16 Besides, LINC00210 also increased the malignancy of thyroid cancer via regulating miR-195-5p/IGF1R. 18 However, the role of LINC00210 in osteosarcoma remains unclear.…”

Section: Introductionmentioning

confidence: 99%

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LncRNA LINC00210 regulated radiosensitivity of osteosarcoma cells via miR‐342‐3p/GFRA1 axis

Wang

et al. 2020

Clinical Laboratory Analysis

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Background: Radiotherapy is an effective strategy for preventing cancer metastasis, including osteosarcoma. However, cancer radioresistance limits the efficiency of radiotherapy. Therefore, it is essential to investigate the mechanism of osteosarcoma radioresistance. Methods: The osteosarcoma tissues and adjacent healthy tissues were collected from 53 osteosarcoma patients. The expression of LINC00210, miR-342-3p, and GFRA1 mRNA were determined using qRT-PCR. Cell viability, cell apoptosis, and cell surviving fraction were determined by MTT assay, flow cytometry, and colony formation assay, respectively. Western blot was performed to detect the protein levels. Luciferase assay was conducted to verify the relationship between LINC00210, miR-342-3p, and GFRA1. Results: LINC00210 and GFRA1 were up-regulated, and miR-342-3p was down-regulated in osteosarcoma tissues and cells. The expression of LINC00210 in osteosarcoma was negatively related to miR-342-3p expression and positively associated with GFRA1. Besides, there was a negative correlation between LINC00210 and GFRA1 expression in osteosarcoma. Also, LINC00210 and GFRA1 were up-regulated, and miR-342-3p was down-regulated in osteosarcoma cells exposed to 4 Gy irradiation treatment. Furthermore, either LINC00210 knockdown or miR-342-3p overexpression enhanced the radiosensitivity of osteosarcoma cells. Moreover, LINC00210 increased GFRA1 expression via sponging miR-342-3p. Additionally, LINC00210 knockdown improved the radiosensitivity of osteosarcoma cells by regulating GFRA1 expression via sponging miR-342-3p. Conclusion: LINC00210 modulated the radiosensitivity of osteosarcoma cells via the miR-342-3p/GFRA1 axis, making LINC00210 a novel target for improving radiotherapy efficiency in osteosarcoma.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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LncRNA LINC00210 regulated radiosensitivity of osteosarcoma cells via miR‐342‐3p/GFRA1 axis

Wang

et al. 2020

Clinical Laboratory Analysis

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miR-328-5p Induces Human Intervertebral Disc Degeneration by Targeting WWP2

Yan

Zhang

et al. 2022

Oxidative Medicine and Cellular Longevity

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Intervertebral disc degeneration (IDD) development is regulated by miRNA, including inflammatory reactions, cell apoptosis, and degradation of extracellular matrix. Nucleus pulposus cells apoptosis has a absolute influence in the development of IDD. This experiment explores the mechanism of miR-328-5p regulating IDD. Through the analysis of miRNA and mRNA microarray database, we screened the target genes miR-328-5p and WWP2. We verified the expression of miR-328-5p, WWP2, and related apoptotic genes in normal and degenerative nucleus pulposus tissues by qRT-PCR. The expressions of WWP2, Bcl-2, and Bax were detected by qRT-PCR and western blot after transfection to nucleus pulposus cell. The nucleus pulposus cell proliferation and apoptosis after transfection were confirmed by CCK8 and flow cytometry. Luciferase reporter assay and bioinformatics analyzed the targeting relationship between miR-328-5p and WWP2. Firstly, the qRT-PCR experiments confirmed the significant increase of miR-328-5p expression, while significant reduction of WWP2 in a degenerative tissues compared to the normal tissues. Surprisingly, miR-328-5p expression was positively, while that of WWP2 negatively correlated with the degeneration grade of IDD. And we also identified the high expression of Bax and Caspase3, while low expression of Bcl-2 in a degenerative tissues. After miR-328-5p mimic transfected into nucleus pulposus cell, qRT-PCR and western blot confirmed that WWP2 and Bcl-2 expressions were downregulated, while Bax and Caspase3 expressions were upregulated, and the same results were obtained by knocking down WWP2. CCK8 and flow cytometry confirmed that miR-328-5p inhibited the proliferation and induced apoptosis of nucleus pulposus cells. WWP2 is a target gene of miR-328-5p by bioinformatics and luciferase reporter assay. In summary, miR-328-5p targets WWP2 to regulate nucleus pulposus cells apoptosis and then participates in the development of IDD. Furthermore, this study may provide new references and ideas for IDD treatment.

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Regulatory roles of noncoding RNAs in intervertebral disc degeneration as potential therapeutic targets (Review)

et al. 2022

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Intervertebral disc degeneration (IDD) is the leading cause of lower back pain, which is one of the primary factors that lead to disability and pose a serious economic burden.The key pathological processes involved are extracellular matrix degradation, autophagy, apoptosis, and inflammation of nucleus pulposus cells. Non-coding RNAs (ncRNAs), including microRNAs, long ncRNAs and circular RNAs, are key regulators of the aforementioned processes. ncRNAs are differentially expressed in tissues of the intervertebral disc between healthy individuals and patients and participate in the pathological progression of IDD via a complex pattern of gene regulation. However, the regulatory mechanisms of ncRNAs in IDD remain unclear. The present review summarizes the latest insights into the regulatory role of ncRNAs in IDD and sheds light on potentially novel therapeutic strategies for IDD that may be implemented in the future. Contents1. Introduction 2. ncRNAs in ECM degradation 3. ncRNAs in autophagy and apoptosis 4. ncRNAs in inflammation 5. Conclusion

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LINC00210 plays oncogenic roles in non‑small cell lung cancer by sponging microRNA‑328‑5p

Cited by 3 publications

References 31 publications

LncRNA LINC00210 regulated radiosensitivity of osteosarcoma cells via miR‐342‐3p/GFRA1 axis

LncRNA LINC00210 regulated radiosensitivity of osteosarcoma cells via miR‐342‐3p/GFRA1 axis

miR-328-5p Induces Human Intervertebral Disc Degeneration by Targeting WWP2

Regulatory roles of noncoding RNAs in intervertebral disc degeneration as potential therapeutic targets (Review)

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