LINC00355:8 promotes cell proliferation and migration with invasion via the MiR-6777-3p/Wnt10b axis in Hepatocellular Carcinoma

Abstract

Background: Recent studies have reported that various long non-coding RNAs (lncRNAs) promote hepatocellular carcinoma (HCC) progression, and our previous study indicated that lncRNA LINC00355:8 is overexpressed in HCC. However, the role of LINC00355:8 in HCC is unclear. The primary aim of this study was to explore the biological role of LINC00355:8 in HCC. Methods: Microarray analysis was performed to explore the aberrantly expressed lncRNAs in HCC compared with precancerous tissues. Real-time PCR and in situ hybridization were used to investigate the expression of LINC00355:8 in HCC tissues. CCK8, EdU, colony formation, wound healing and transwell assays were performed to analyse cell proliferation, migration and invasion. A xenograft tumour model was established to analyse the effect of LINC00355:8 on tumour growth in vivo. Luciferase assays were utilized to explore the binding sites between miR-6777-3p and other genes, such as LINC00355:8 and Wnt10b. After cell transfection, the protein expression levels of Wnt10b, β-catenin, E-cadherin, N-cadherin, c-Myc and Snail were determined by Western blotting. Results: The present study revealed that LINC00355:8 was significantly upregulated in HCC, promoted HCC cell proliferation, migration and invasion in vitro and enhanced tumour growth in vivo. LINC00355:8 regulated miR-6777-3p expression by acting as a ceRNA, and the expression of Wnt10b was negatively modulated by miR-6777-3p. Moreover, LINC00355:8 could activate the Wnt/β-catenin signalling pathway and promote EMT progression by inhibiting the miR-6777-3p/Wnt10b interaction in HCC. Conclusion: Our findings indicate that LINC00355:8 activates Wnt10b and promotes HCC progression via the suppression of miR-6777-3p, which may provide novel therapeutic targets for HCC.

Identification of miRNA and Their Regulatory Effects Induced by Total Flavonoids From Dracocephalum moldavica in the Treatment of Vascular Dementia

“…Among these miRNAs, miR-6784-3p, miR-6762-3p, miR-4747-3p, and miR-4746-3p were reported for the first time. Other significantly expressed miRNAs, such as miR-6777-3p, miR-3184-3p, miR-3184-5p, miR-4667-5p, and miR-6875-5p, closely implicated with the occurrence and development of a variety of tumors ( Miyamoto et al, 2015 ; Akazawa et al, 2019 ; Hindle et al, 2019 ; Zhou et al, 2020 ; Liu et al, 2021 ), were identified for the first time in VaD, suggesting a novel potential regulatory role in the pathology of this disease. miR-3675-3p is a unique biomarker representing a target in the inhibition of cell apoptosis through the MAPK signaling pathway ( Zhang et al, 2020 ).…”

Transcriptome analysis reveals potential marker genes for diagnosis of Alzheimer’s disease and vascular dementia

“…Furthermore, PPP2CA as a candidate gene that it may affect the risk of AD ( Vazquez-Higuera et al, 2011 ). NCEH1 and WNT10B, and ATP1A3 have been rarely reported to be associated with AD, but WNT10B has an important role in progression of colorectal cancer ( Shi et al, 2019 ) and hepatocellular carcinoma ( Zhou et al, 2020 ). Therefore, suggesting that WNT10B, ITPR1, GABBR2, ATP1A3, NCEH1, MAP2K4, PPP2CA, and GRIA4 may play a vital role in AD and VD, while also need more studies to further validate the expression of hub genes.…”

LINC_00355 promotes gastric cancer progression by upregulating PHF19 expression through sponging miR-15a-5p

“…For instance, LINC_00355 promoted HNSCC progression through binding miR-195 [ 12 ]. In hepatocellular carcinoma (HCC), LINC_00355 acted as a ceRNA to sponge miR-6777-3p and further promoted HCC progression [ 19 ]. These findings indicate that LINC_00355 regulates cancer development in multiple pathways and highlights the important role of LINC_00355 in cancer progression.…”