LINC00467 enhanced the proliferative, migratory and invasive ability of breast cancer cells by targeting miR-18a/b-5p/MAPK4 axis

Abstract

Cell linesHuman BC cell lines (MCF7, MDA-MB-468, MDA-MB-231, SK-BR-3) and MCF10A (both; ATCC, Rockville, Maryland), were routinely cultured at 37 o C with 5% CO 2 in DMEM. 10% FBS-contusing antibiotics were acquired for supplementing DMEM. qRT-PCRAccording to the protocol, 2 ug of total RNA was extracted with Trizol reagent and then treated for cDNA LINC00467 enhanced the proliferative, migratory and invasive ability of breast cancer cells by targeting miR-18a/b-5p/MAPK4 axis