LINC00511/miRNA-143-3p Modulates Apoptosis and Malignant Phenotype of Bladder Carcinoma Cells via PCMT1

Abstract

Bladder cancer has easy recurrence characteristics, but its occurrence and development mechanism are still unclear. Non-coding RNA is a kind of RNA that exists widely and cannot be translated into proteins, which has played a key role in the regulation of biological functions of tumor cells. However, the regulation mechanism of non-coding RNA on bladder tumors is not fully understood. By microarray analysis and database analysis, we found that LINC00511 was significantly highly expressed in bladder cancer. The expressions of LINC00511, miR-143-3p, and PCMT in bladder cancer tissues and cells were detected by quantitative reverse transcription–polymerase chain reaction. The relationship between the expressions of miR-143-3p and PCMT1 and the clinicopathological parameters of the tumor was analyzed. The proliferation and invasion of bladder cancer cells were detected by MTT assay and Transwell assay. The expression levels of E-cadherin and vimentin in bladder cancer cells were detected by Western blot. Cell apoptosis was detected by flow cytometry. In vivo, TCCSUP or SW780 cells were inoculated into BALB/c nude mice to detect tumor volume and weight. Bioinformatics and dual luciferase reporter gene were used to analyze the relationship between LINC00511 and miR-143-3p and its downstream target gene PCMT1. The results showed that LINC00511 could target miR-143-3p/PCMT1 to regulate the proliferation, migration, and apoptosis of bladder cancer TCCSUP or SW780 cells and promote the occurrence and development of bladder cancer.

Crosstalk of Histone and RNA Modifications Identified a Stromal-Activated Subtype with Poor Survival and Resistance to Immunotherapy in Gastric Cancer

“…Interestingly, three EMRLs (PAXIP1-AS2, MNX1-AS1, and PVT1) were co-regulated by the four modification types ( Figure 3B ). LINC00511, previously reported as an oncogene in several solid tumors ( Wu et al, 2020 ; Peng et al, 2021 ; Dong et al, 2021 ), was significantly modified with H3K4me1 ( Supplementary Figure S2 ) and simultaneously overexpressed in the three GC cell lines ( Figure 3F ). Similarly, concomitant histone modification and overexpression were observed in LINC01091 ( Supplementary Figure S2 ).…”

Long Noncoding RNA LINC02470 Sponges MicroRNA-143-3p and Enhances SMAD3-Mediated Epithelial-to-Mesenchymal Transition to Promote the Aggressive Properties of Bladder Cancer

“…In the present study, we report that the interaction between LINC02470–miR-143-3p is a rising novel posttranscriptional regulation that might also result in degradation of both RNA molecules. Recently, potential roles of miR-143 in ceRNA regulation were also noticed, and several other lncRNA–mRNA pairs have been reported in bladder cancer, including the PCAT6–miR-143-3p–PDIA6 axis [ 41 ], LINC00511–miR-143-3p–PCMT1 axis [ 42 ], SNHG1–miR-143-3p–EZH2 axis [ 43 ], MAFG–AS1–miR-143-3p–COX-2 axis [ 44 ], circ_0006332–miR-143–MYBL2 axis [ 45 ], FOXD2–AS1–miR-143–ABCC3 axis [ 46 ], and UCA1–miR-143–HMGB1 axis [ 47 ]. This is also the first study to address miR-143(-3p)-regulated ceRNA in EMT process regulation.…”

Molecular Classification of Breast Cancer Utilizing Long Non-Coding RNA (lncRNA) Transcriptomes Identifies Novel Diagnostic lncRNA Panel for Triple-Negative Breast Cancer

“…In addition to this, the silencing of LINC00511 expression suppressed cell viability, proliferation, migration, and invasion, and accelerated the apoptosis of glioma cells via a suggested miR-15a-5p/AEBP1 axis [ 36 ]. Other studies have associated LINC00511 with breast cancer [ 37 ], hepatocellular carcinoma [ 38 ], and bladder carcinoma [ 39 ].…”