LINC00641 hinders the progression of cervical cancer by targeting miR‐378a‐3p/CPEB3

Abstract

Background LINC00641 was found to act in anti‐tumor manner in several types of cancers. Nonetheless, the detailed functions of LINC00641 have not been determined in cervical cancer (CC). Methods The expression of LINC00641, miR‐378a‐3p and CPEB3 was examined using a quantitative reverse transcriptase‐polymerase chain reaction. The relationships between LINC00641 and its downstream mechanism were illustrated by RNA pull‐down and luciferase reporter experiments. Results LINC00641 was found to be under‐expressed in CC cell lines. By overexpressing LINC00641, cell proliferative, migratory and invasive abilities, as well as epithelial mesenchymal transition (EMT) characteristics, were inhibited, whereas the rate of apoptosis was increased. Next, a starBase search (http://starbase.sysu.edu.cn) was applied to select microRNAs that had binding sequences with LINC00641. By up‐regulating LINC00641 expression, miR‐378a‐3p expression displayed the strongest decline. Moreover, miR‐378a‐3p was found to be up‐regulated in CC cell lines. In addition, LINC00641 hindered the progression of CC by decreasing miR‐378a‐3p expression. CPEB3 was discovered as a downstream target of miR‐378a‐3p and was under‐expressed in CC cells. Furthermore, knockdown of CPEB3 could counter the influence of an overexpression of LINC00641 with respect to CC progression. Conclusions LINC00641 suppressed the progression of CC by targeting miR‐378a‐3p/CPEB3, suggesting that LINC00641 may have positive therapeutic impact for treatment for CC.

JPX and LINC00641 ncRNAs expression in prostate tissue

“…reported that LINC00641 is acting as a tumor suppressor in bladder and glioblastoma patients, and a decrease in its expression was observed ( 50 51 ). Also, concerning breast and cervical cancer similar results have been attained ( 52 53 ). Therefore, all the above studies suggest that the function of LINC00641 may vary depending on the type of cancer.…”

DCTPP1, an Oncogene Regulated by miR-378a-3p, Promotes Proliferation of Breast Cancer via DNA Repair Signaling Pathway

“…Herein, we demonstrated that miR-378a-3p serves as an upstream modulator of DCTPP1 and, using luciferase studies, found that it directly targets DCTPP1. MiR-378a-3p is dysregulated in some cancers, where it has tumor-suppressor functions (40)(41)(42). We find that miR-378a-3p is downregulated in BRCA tissue, and its expression is negatively linked to tumor size.…”

Identification and validation of RNA binding protein-associated prognostic model for Neuroblastoma

“…CPEB3 can disrupt the crosstalk between cancer cells and tumor-associated macrophages through IL-6R/STAT3 signaling, thereby inhibiting epithelial-mesenchymal transition. Studies have found that CPEB3 is related to the tumorigenesis and development of glioma [25], high-grade serous ovarian cancer [26], colorectal cancer [27], hepatocellular carcinoma [28], cervical cancer [29]. Based on the two hub RBPs trained by the TARGET cohort, multi-step Cox regression analysis produced a riskscore model that can predict the prognosis of NB.…”