LINC00665 induces gastric cancer progression through activating Wnt signaling pathway

Abstract

Long noncoding RNAs (lncRNAs) are recently recognized as noteworthy regulators of different tumors, counting gastric cancer (GC). Lately, long intergenic noncoding RNA (LINC) 00665 has been verified to display significant parts in several cancers. Be that as it may, its role and mechanism in GC movement still stay uninvestigated. As of now, we observed LINC00665 was obviously GC cells (MKN28, BGC‐823, SGC7‐901, AGS, HGC‐27) in comparison to GES‐1 cells, which was identified as human normal gastric epithelial cells. Then, LINC00665 was obviously downregulated in GC cells including AGS and BGC‐823 cells. Loss of LINC00665 greatly repressed AGS and BGC‐823 cell survival and cell expansion. Moreover, GC cell apoptosis was significantly induced by the loss of LINC00665. For another, we found that the GC cell cycle was also captured in G1 and G2 phases. The experiments on cell migration and invasion indicated that knockdown of LINC00665 restrained GC cell migration and invasion. Modifications in Wnt signaling are closely associated with the development of cancers. Here, we found that Wnt signaling was significantly inactivated by the silence of LINC00665 in GC cells. β‐catenin and cyclinD1 were restrained whereas GSK‐3β was induced by the inhibition of LINC00665 in GC cells. Furthermore, we confirmed the impact of LINC00665 in vivo using xenograft models. Taken these together, we indicated that LINC00665 could function as a novel biomarker in GC progression.

LINC00665 promotes the progression of acute myeloid leukemia by regulating the miR-4458/DOCK1 pathway

“…LINC00665/miR-4458/DOCK1 interactome identification. LINC00665 has been reported to be a tumor promoter in various human cancers, such as lung cancer 31,32 , gastric cancer 17,33 , hepatocellular carcinoma [34][35][36] , and breast cancer 16,37 . However, its effects on AML cells remain unexplored.…”

LINC00665 promotes Ovarian Cancer progression through regulating the miRNA-34a-5p/E2F3 axis

“…With the development of high-throughput sequencing, dysfunctional lncRNAs are believed as key events for tumor development [21]. Recent studies have shown that LINC00665 exerts a carcinogenic role in many types of tumors [17][18][19].…”

“…LINC00665 triggers lung carcinoma progression by sponging miR-98 and thus mediates the ERK signaling [16]. In addition, LINC00665 is also involved in the progression of gastric cancer and breast cancer [17][18][19]. Its biological function in OC is largely unclear.…”

Linc00665 Can Predict the Response to Cisplatin-Paclitaxel Neoadjuvant Chemotherapy for Breast Cancer Patients

“…Yu et al reported that Linc00665 deletion can inhibit the migration and invasion of triple negative breast cancer cell lines MDA-MB-231 and BT549 ( 12 , 31 ). Gao et al elucidated that Linc00665 knockdown can inhibit the migration and invasion of gastric cancer cell lines AGS and BGC‐823 ( 9 ). Wang et al also reported that Linc00665 silencing can significantly reduce the invasive ability of gastric cancer cell lines AGS and MGC-803 ( 32 ).…”