LINC00665 Stimulates Breast Cancer Progression via Regulating miR-551b-5p

Abstract

Introduction Long intergenic non-protein coding RNA 665 (LINC00665) has been revealed to contribute cancer progression in many cancer types including liver and gastric cancer. However, the roles of LINC00665 in breast cancer (BC) remain to be explored. Methods We explored LINC00665 expression in BC tissues and normal tissues at GEPIA. Then, its expression in BC cells (HCC-1937, MDA-MB-231, and MCF-7) and normal cells (MCF10A) was analyzed with qRT-PCR. In addition, the mechanisms of LINC00665 in BC were explored using bioinformatic analyses, luciferase activity reporter assay, RNA pull-down assay, and rescue experiments. Results We showed LINC00665 expression was significantly increased in both BC tissues and cells. The knockdown of LINC00625 significantly inhibits BC cell growth and promotes cell apoptosis in vitro, while the overexpression of LINC00625 has the opposite effects on BC progression. LINC00665 could affect BC progression via regulating miR-551b-5p. Discussion Taken together, our study showed that the LINC00665/miR-551b-5p axis was involved in the progression of BC.

The Profile of MicroRNA Expression and Potential Role in the Regulation of Drug-Resistant Genes in Cisplatin- and Paclitaxel-Resistant Ovarian Cancer Cell Lines

“…miR-551b-3p, expression level was also significantly downregulated in the gastric cancer tissues, which correlated with the degree of differentiation (i.e., tumor grade), TNM stage, and lymph node metastasis [ 56 ]. Downregulation of miR-551b-5p was reported in breast cancer cell lines and tissues and was an indicator of poor overall survival [ 57 ]. We observed downregulation of both miRs in PAC-resistant cell lines while in others during tumor progression.…”

The ncRNA-Mediated Overexpression of Ferroptosis-Related Gene EMC2 Correlates With Poor Prognosis and Tumor Immune Infiltration in Breast Cancer

“…For example, knockdown of LINC00665 significantly inhibits the growth of breast cancer cells in vitro , significantly weakens the migration and invasion abilities, reduces the expression of EMT-related genes, and promotes apoptosis. By contrast, the overexpression of LINC00665 can promotes breast cancer progression ( 11 , 33 ).…”

The Emerging Roles of LINC00665 in Human Cancers

“…Furthermore, LINC00665 expression was significantly associated with the tumor size and TNM stage, but not with the age of the breast cancer patients ( Lv et al, 2020 ). In addition, Qi et al conducted the Kaplan-Meier survival analysis to explore the correlation between LINC00665 expression and the prognosis of breast cancer patients ( Qi et al, 2021 ). They found that high expression of LINC00665 could predict poor overall survival of breast cancer patients ( Qi et al, 2021 ).…”

“…In addition, Qi et al conducted the Kaplan-Meier survival analysis to explore the correlation between LINC00665 expression and the prognosis of breast cancer patients ( Qi et al, 2021 ). They found that high expression of LINC00665 could predict poor overall survival of breast cancer patients ( Qi et al, 2021 ). Studies have reported that LINC00665 played a vital role in breast cancer progression, and LINC00665 Knockdown inhibited breast cancer cells proliferation, migration, and invasion but promoted apoptosis ( Ji et al, 2020 ; Lv et al, 2020 ; Zhou et al, 2020 ; Qi et al, 2021 ).…”

“…They found that high expression of LINC00665 could predict poor overall survival of breast cancer patients ( Qi et al, 2021 ). Studies have reported that LINC00665 played a vital role in breast cancer progression, and LINC00665 Knockdown inhibited breast cancer cells proliferation, migration, and invasion but promoted apoptosis ( Ji et al, 2020 ; Lv et al, 2020 ; Zhou et al, 2020 ; Qi et al, 2021 ). Moreover, Ji et al (2020) examined the expression of mesenchymal and epithelial markers by qRT-PCR, Western blot, and immunofluorescence.…”