LINC00852 Promotes Lung Adenocarcinoma Spinal Metastasis by Targeting S100A9

Abstract

Background: Lung adenocarcinoma has a strong tendency to develop into bone metastases, especially spinal metastases (SM). Long noncoding RNAs (lncRNAs) play critical roles in regulating several biological processes in cancer cells. However, the mechanisms underlying the roles of lncRNAs in the development of SM have not been elucidated to date.Methods: Clinical specimens were collected for analysis of differentially expressed lncRNAs. The Kyoto Encyclopedia of Genes and Genomes (KEGG) was used to examine the effects of these genes on pathways. RNA pull-down was utilized to identify the targeting protein of lncRNAs. The effects of lncRNA on its target were detected in A549 and SPCA-1 cells via perturbation of the lncRNA expression. Oncological behavioral changes in transfected cells and phosphorylation of kinases in the relevant pathways, with or without inhibitors, were observed. Further, tumorigenicity was found to occur in experimental nude mice.Results: LINC00852 and the mitogen-activated protein kinase (MAPK) pathway were found to be associated with SM. Moreover, the LINC00852 target S100A9 had a positive regulatory role in the progression, migration, invasion, and metastasis of lung adenocarcinoma cells, both in vitro and in vivo. Furthermore, S100A9 strongly activated the P38 and REK1/2 kinases, and slightly activated the phosphorylation of the JNK kinase in the MAPK pathway in A549 and SPCA-1 cells.Conclusion: LINC00852 targets S100A9 to promote progression and oncogenic ability in lung adenocarcinoma SM through activation of the MAPK pathway. These findings suggest a potential novel target for early intervention against SM in lung cancer.

Exosome‐transmitted linc00852 associated with receptor tyrosine kinase AXL dysregulates the proliferation and invasion of osteosarcoma

“…24,25 Until now, only one study has been conducted on linc00852, which promote lung adenocarcinoma progression and spinal metastasis by activating MAPK pathway. 26 In this study, we have proven that exosomal linc00852 plays as a pivotal identified intercellular messenger in osteosarcoma. Exosomes are not only a cell-cell communication regulator but also an important mediator in the process of vascular remodeling, playing a mediating role in tumor microenvironment and promoting tumor metastasis.…”

Weighted Gene Co-Expression Network Analysis Identifies Critical Genes in the Development of Heart Failure After Acute Myocardial Infarction

“…In our study, through transcriptomics-based screening, we found that the 13 identified lncRNAs exhibited close interactions with genes in the blue module. Studies have revealed that AATBC (Zhao et al, 2015), ADAMTSL4-AS1 (Annunziato et al, 2019), EPB41L4A-AS1 (Liao et al, 2019), MIA-RAB4B (Thean et al, 2017), MIR4697HG (Zhang et al, 2017a), GAS5 (Ni et al, 2019), SNHG12 (Tamang et al, 2019), MSX2P1 (Qiao et al, 2018), and LINC00852 (Liu et al, 2018b) are related to tumorigenesis and tumor progression in multiple cancers, such as bladder, breast, colorectal, and ovarian. Apart from the nine lncRNAs identified in biological experiments, LOC254896 (Singh et al, 2018), LINC01000 (Mitchell et al, 2017), LINC00893 (Li et al, 2019a), and LINC00537 (Li et al, 2017b) were also reported to be significantly differentially expressed between diseased and normal tissues in some transcriptional profiling analyses.…”

Serum lnc34a is a Potential Prediction Biomarker for Bone Metastasis in Hepatocellular Carcinoma Patients

“…Liu P et. al indicated that LINC00852 promotes lung adenocarcinoma spinal metastasis by targeting S100A9 [37]. Although lncRNAs have played an emerging role in bone metastasis from other cancers, there are no studies assessing serum lncRNAs in cancer patients with bone metastasis.…”