LINC00958 facilitates cervical cancer cell proliferation and metastasis by sponging miR‐625‐5p to upregulate LRRC8E expression

Wang, Lifeng; Zhong, Yanbo; Yang, Baohua; Zhu, Yunheng; Zhu, Xiuxiang; Xia, Ziyin; Xu, Jun; Xu, Ling

doi:10.1002/jcb.29472

Cited by 41 publications

(32 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…An increasing amount of evidence suggests that dysregulation of lncRNAs are closely implicated in the pathogenesis, development and metastasis of CRC (22,23). Emerging evidence confirms that LINC00958 expression is notably overexpressed in cervical cancer cells, and LINC00958 contributes to the proliferation and metastasis of cervical cancer cells (24). In support, a previous study highlighted the importance of LINC00958 in the occurrence of glioma (25).…”

Section: Discussionmentioning

confidence: 85%

Downregulation of LINC00958 inhibits proliferation, invasion and migration, and promotes apoptosis of colorectal cancer cells by targeting miR‑3619‑5p

et al. 2020

View full text Add to dashboard Cite

The aberrant expression of long non-coding RNAs (lncRNAs), including LINC00958, has been demonstrated in several types cancers. The present study aimed to investigate the role of LINC00958 in colorectal cancer (CRC) and identify the possible underlying mechanisms. The expression of LINC00958 and microRNA (miR)-3619-5p was detected in several human CRC cell lines using reverse transcription-quantitative PCR. Then, short hairpin RNA (shRNA)-LINC00958 was transfected into the cells. The results revealed that the expression of LINC00958 was notably upregulated, whereas miR-3619-5p was downregulated in CRC cells. Transfection with shRNA-LINC00958 inhibited the proliferation, invasion and migration of CRC cells. Moreover, the rate of apoptosis was enhanced, accompanied by a decrease in the expression of Bcl-2 and an increase in the expression of Bax and caspase-3. A luciferase reporter assay was conducted to verify the target binding site between LINC00958 and miR-3619-5p. The luciferase reporter assay confirmed that miR-3619-5p could be directly targeted by LINC00958. Furthermore, the miR-3619-5p inhibitor reversed the effects of LINC00958 silencing on proliferation, invasion, migration and apoptosis. Taken together, the findings suggest that the downregulation of LINC00958 suppresses the proliferation, invasion and migration, and promotes the apoptosis of CRC cells by targeting miR-3619-5p in vitro, which provides a theoretical basis and therapeutic strategy for the treatment of CRC.

show abstract

Section: Discussionmentioning

confidence: 85%

Downregulation of LINC00958 inhibits proliferation, invasion and migration, and promotes apoptosis of colorectal cancer cells by targeting miR‑3619‑5p

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Several studies have suggested that lncRNAs, including BLACAT2, regulates cancer progression by sponging miRNAs. For instance, BLACAT2 has been shown to facilitate cervical cancer cell proliferation and metastasis by sponging miR-625-5p, which upregulates the expression of LRRC8E [23]. Similarly, it was verified that the silencing of BLACAT2 prevents tumor initiation of pancreatic cancer by acting as a sponge of microRNA-330-5p to down-regulate PAX8 [24].…”

Section: Discussionmentioning

confidence: 95%

Longnon-coding RNA BLACAT2 promotes gastric cancer progression via the miR-193b-5p/METTL3 pathway

Hu¹,

Kong²,

Huang³

et al. 2021

J. Cancer

View full text Add to dashboard Cite

Gastric cancer is one of the leading prevalent and malignant cancers worldwide, especially in east Asia. However, the in-depth molecular mechanism underlying gastric cancer progression remains uncertain. Recently, studies have identified that long non-coding RNA (lncRNA) could play critical roles in the tumorigenesis of multiple types of cancer. Studies on long non-coding RNA BLACAT2 have proven that it participates in bladder cancer and colorectal cancer regulation and was identified as highly expressed using the cBioPortal for Cancer Genomics in gastric cancer. However, the precise function of lncRNA-BLACAT2 in the carcinogenesis and progression of gastric cancer remains largely unexplored. Our study discovered that lncRNA-BLACAT2 was significantly upregulated in gastric cancer. Different studies have illustrated that BLACAT2 promoted gastric cancer progression through regulating proliferation, migration, invasion, and apoptosis in terms of biological function. Furthermore, BLACAT2 was verified to perform its function through interaction with miR-193b-5p using a luciferase reporter assay. On the other hand, MiR-193b-5p specific inhibitor treatment reversed the inhibitory effect of BLACAT2 on cell biological functions. Additional studies also discovered that Methyltransferase Like 3 (METTL3) was the downstream target of miR-193b-5p. Subsequently, restoration of METTL3 eliminated the suppressive effect of proliferation or the promotive effect of apoptosis caused by BLACAT2 knockdown. To sum up, these experimental results demonstrated that BLACAT2 acted as an oncogene in gastric cancer progression through the regulation of the miR-193b-5p/METTL3 pathway, hence providing new insights regarding the pathogenesis of gastric cancer.

show abstract

“…A recent study showed that LINC00958 is involved in the carcinogenesis of bladder cancer [12]. The high expression of LINC00958 has also been shown to promote the occurrence and development of certain cancers, including bladder cancer, oral cancer, pancreatic cancer, gastric cancer, cervical cancer and glioma [13][14][15][16][17][18]. However, the relevant mechanism of LINC00958 in EC, especially ESCC, has not yet been determined.…”

Section: Introductionmentioning

confidence: 99%

LINC00958 promotes proliferation, migration, invasion, and epithelial-mesenchymal transition of oesophageal squamous cell carcinoma cells

et al. 2021

View full text Add to dashboard Cite

Oesophageal cancer is one of the deadliest cancers in the world. Oesophageal squamous cell carcinoma (ESCC) is the most prevalent histological type of oesophageal cancer. Oesophageal cancer has a poor prognosis because of its invasiveness. Thus, it is especially important to seek effective treatment methods. Research indicates that long non-coding RNAs (lncRNAs) play a significant role in the occurrence and development of oesophageal cancer. The aim of this study was to describe the role of LINC00958 in ESCC. Bioinformatics and real-time quantitative polymerase chain reaction (RT-qPCR) methods were utilized to predict and verify the expression of LINC00958 in ESCC. Related functional experiments, including cell proliferation, migration and invasion, were performed. In addition, a western blot and a dual luciferase reporter gene experiment were used to study the detailed carcinogenic mechanism of LINC00958. The results indicated there was a high expression of LINC00958 in ESCC, which promoted proliferation, migration, invasion and Epithelial–Mesenchymal Transition (EMT) of ESCC cells, and this effect may be via regulating miR-510-5p.

show abstract

LINC00958 facilitates cervical cancer cell proliferation and metastasis by sponging miR‐625‐5p to upregulate LRRC8E expression

Cited by 41 publications

References 24 publications

Downregulation of LINC00958 inhibits proliferation, invasion and migration, and promotes apoptosis of colorectal cancer cells by targeting miR‑3619‑5p

Downregulation of LINC00958 inhibits proliferation, invasion and migration, and promotes apoptosis of colorectal cancer cells by targeting miR‑3619‑5p

Longnon-coding RNA BLACAT2 promotes gastric cancer progression via the miR-193b-5p/METTL3 pathway

LINC00958 promotes proliferation, migration, invasion, and epithelial-mesenchymal transition of oesophageal squamous cell carcinoma cells

Contact Info

Product

Resources

About