Linc01018 / hsa-mir-182-5p / ADH4 Axis Study on the Role and Mechanism in the Occurrence and Development of Liver Cancer

Abstract

Background To construct the lncRNA-miRNA-mRNA axis based on the study of molecular oncology, to explore the role and mechanism of this axis in the occurrence and development of liver cancer, so as to provide a new channel for the treatment of liver cancer. Methods Using public online databases to establish lncRNA-miRNA-mRNA ceRNA regulation network, after which using QPCR and other experimental techniques to verify that this axis is established and the mechanism of participating in the development of liver cancer. Results It can be concluded from database mining that the expressions of hsa-miR-182-5p and ADH4 are negatively correlated in hepatocellular carcinoma, and LINC01018 is also negatively correlated hsa-miR-182-5p-ADH4, indicating that LINC01018, hsa-miR-182-5p and ADH4 are strongly correlated. Constitute the regulatory axis to participate in the occurrence and development tendency of tumors. LINC01018 regulates ADH4 to inhibit LIHC cell growth by inhibiting hsa-miR-182-5p, providing a feasible theoretical basis for the treatment of HCC.The regulatory axis may also regulate the occurrence and development tendency in liver cancer by adjusting the expression levels of key proteins and phosphorylation proteins in GO and KEGG signaling pathways. Conclusions In this study, it was found that LINC01018/hsa-miR-182-5p/ADH4 ceRNA regulatory axis exists in the human body, and this axis has the possibility of becoming an immune checkpoint inhibitor for liver cancer, which is regarded as a new entry point in the diagnosis and therapy of liver cancer.