LINC01089 blocks malignant progression of thyroid cancer by binding miR-27b-3p to enhance the FBLN5 protein level

Pan, Yongqin; Huang, Kunsong; Chong, Tsz-Hong; Li, Jinyi

doi:10.1007/s12672-022-00580-4

Cited by 4 publications

(3 citation statements)

References 36 publications

(43 reference statements)

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“…It is well-established that cancer-suppressive lncRNAs can interact with many cancer-promoting miRNAs and inhibit their functions [ 22 ]. Here, we performed subcellular fractionation to determine the cellular localization of PGM5-AS1.…”

Section: Resultsmentioning

confidence: 99%

LncRNA PGM5-AS1 inhibits non-small cell lung cancer progression by targeting miRNA-423-5p/SLIT2 axis

Wang,

Ye,

Dang

et al. 2024

Cancer Cell Int

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Non-small cell lung cancer (NSCLC) is a common and aggressive primary malignancy worldwide. Dysregulation of long non-coding RNAs (lncRNAs) has been shown to play an essential regulatory role in multiple cancers. However, the role of PGM5-AS1 in NSCLC remains unclear. Here, we found that PGM5-AS1 was down-regulated in NSCLC tissues and cells. Furthermore, reduced PGM5-AS1 expression levels were associated with larger tumor size, positive lymph node metastasis, advanced TNM stage and worse prognosis. We found that overexpression of PGM5-AS1 inhibited cell proliferation and metastasis, and induced apoptosis and G0/G1 cell cycle arrest in NSCLC cell lines. Using dual luciferase gene reporter and RNA immunoprecipitation assays, we confirmed that miR-423-5p interacted with PGM5-AS1, and that their expression levels were negatively correlated in NSCLC tissues. miR-423-5p was also found to reverse PGM5-AS1-induced malignant biological behavior. Moreover, we identified slit guidance ligand 2 (SLIT2) as a target gene of miR-423-5p. Using a dual luciferase gene reporter assay, we confirmed the regulatory relationship between SLIT2 and miR-423-5p and demonstrated that their expression levels were negatively correlated. Our rescue experiments showed that SLIT2 knockdown reversed miR-423-5p-mediated effects. Overall, this study identifies PGM5-AS1 as a potential prognostic biomarker for NSCLC and shows that PGM5-AS1 suppresses NSCLC development by regulating the miR-423-5p/SLIT2 axis.

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Section: Resultsmentioning

confidence: 99%

LncRNA PGM5-AS1 inhibits non-small cell lung cancer progression by targeting miRNA-423-5p/SLIT2 axis

Wang,

Ye,

Dang

et al. 2024

Cancer Cell Int

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“…Hsa-miR-27a-3p has been verified to be involved in the development of PTC [ 30 ]. LINC01089 can block the malignant progression of TC by binding hsa-miR-27b-3p to increase the level of FBLN5 protein [ 31 ]. TGFBR3 expression is down-regulated in PTC tissues and is associated with OS of patients [ 32 ].…”

Section: Discussionmentioning

confidence: 99%

Reveal the potential molecular mechanism of circRNA regulating immune-related mRNA through sponge miRNA in the occurrence and immune regulation of papillary thyroid cancer

Wang,

Shen,

Yan

et al. 2023

Annals of Medicine

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Background Papillary thyroid cancer (PTC) is the most common endocrine malignant tumour. The purpose of this study was to explore the potential molecular mechanism of circRNA regulating immune-related mRNA through sponge miRNA in the occurrence and immune regulation of PTC. Methods All data were downloaded from public databases, such as GEO, Immport and TCGA. Differentially expressed (DE) mRNAs (DEmRNAs), DEmiRNAs and DEcircRNAs were identified using metaMA and limma packages. Subsequently, immune-related DEmRNAs were screened, and circRNA-miRNA-mRNA (ceRNA) regulatory network was constructed. In addition, functional annotation, protein-protein interaction (PPI) network construction, immune cell infiltration analysis and Pearson correlation analysis were performed. Finally, qRT-PCR validation and cell experiments were also performed. Results In total, 2962 DEmRNAs, 78 DEmiRNAs and 51 DEcircRNAs were obtained. Subsequently, 195 immune-related DEmRNAs were obtained based on Immport database. Cytokine-cytokine receptor interaction was the only signalling pathway obtained in KEGG analysis. Then, 8 hub immune-related DEmRNAs were identified based on PPI network and CytoHubba plug-in. Subsequently, ceRNA sub-network containing hub immune-related DEmRNAs was extracted from ceRNA regulatory network. In ceRNA sub-network, hsa_circ_0082182-hsa-miR-18b-5p-FGF1/PDGFC, hsa_circ_0016404-hsa-miR-1275-FGF1/CTSB/IL13RA1, hsa_circ_0070100-hsa-miR-27a-3p/hsa-miR-27b-3p-TGFBR3, hsa_circ_0060055/hsa_circ_0038718-hsa-miR-150-3p-CXCL14, hsa_circ_0030427/hsa_circ_0002917-hsa-miR-22-3p-BMP7 and hsa_circ_0030427/hsa_circ_0002917-hsa-miR-125a-5p-LIFR axes were identified. Moreover, FGF1, PDGFC, CTSB, IL13RA1, TGFBR3, CXCL14, BMP7, LIFR, hsa-miR-125a-5p, hsa-miR-1275, hsa-miR-150-3p, hsa-miR-18b-5p and hsa-miR-27b-3p were also found to have good diagnostic accuracy and may be potential novel diagnostic markers for PTC. XCell analysis showed that the levels of immune cell infiltration (including Tregs, HSC, DC and Monocytes) were significantly different between the PTC and the control groups. Knockdown of the expression of hsa_circ_0082182 significantly inhibits the activity, proliferation, migration and invasion of TPC-1 cells. Conclusion Several circRNA-miRNA-mRNA axes identified in this study may be related to the occurrence, progression and survival of PTC. This lays a theoretical foundation for further understanding the molecular mechanism of PTC, and also contributes to clinical management and research.

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“…For example, based on the study of Qian et al in 2021, in lung cancer cells, LNC01089 regulates the miR-301b-3p/STARD13 interaction axis, and through this mechanism, this lncRNA could decrease the migration rate of BC cells(Qian et al, 2021). LNC01089 also regulates the miR-27b-3p/FBLN5 axis in thyroid cancer patients, and this mechanism could suppress the malignant development(Pan et al, 2022)There has been no previous research on the diagnostic utility of LNC01089 across any cancer form, and for the rst time, we showed that this lncRNA has a signi cant diagnostic capability for distinguishing cancer samples from normal samples based on ROC analysis.…”

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confidence: 99%

LNC01089-LINC00963/miR-1244-5p/IGF1 ceRNA axis might regulate FOXO signaling pathway in breast cancer patients: a biomarker discovery investigation

Rezaei,

Marghmaleki,

Boroujeni

et al. 2023

Preprint

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Background Breast carcinoma (BC) ranks as one of the most prevalent illnesses among women, and a variety of factors, including inherited and environmental factors, can impact its start and progression. A variety of biological biomarkers (measurement of enzymes, hormones, and mRNA and microRNA expression patterns) have been identified for the prediction of poor prognosis and diagnosis of BC. In this study, we tried to analyze the expression patterns of mRNAs and long non-coding RNAs (lncRNAs) and find novel biomarkers for diagnosis and prognosis of BC during a systems biology approach. Methods Microarray analysis was performed to find novel potential BC biomarkers. Using miRWalk, lncRRIsearch, STRING, and Cytoscape, non-coding and protein interaction analysis was utilized and visualized. Pathway enrichment and gene ontology analyses were performed to find accurate biological mechanisms of selected RNAs. The correlation of lncRNA and mRNA expression level with the survival rate of BC patients was shown using GEPIA2. Expression level of miRNA was performed using ENCORI. Using qRT-PCR on 50 tumor samples compared to 50 control samples for validation of bioinformatics expression analyses and understanding of diagnosis capability of selected RNAs (using Receiver operating characteristic (ROC) analysis. Results IGF1 expression level had a significant reduction in BC, based on microarray and qRT-PCR experiments. LINC00963 and LNC01089 also have significant decrease in expression level, based on GEPIA2 and qRT-PCR. LNC01089 and LINC00963 could represent suitable BC diagnostic (depending on ROC analysis) and prognosis (clinicopathological analysis) biomarkers. The two mentioned lncRNAs have direct interaction with IGF1 mRNA. miR-1244-5p as a potential up-regulated oncogene of BC suppresses the expression level of LNC01089, LINC00963, and IGF1. IGF1 is a key modulator of the FOXO signaling pathway. The mentioned RNAs have a significant correlation with clinicopathological features of BC patients, including age, lymph node metastasis, and menopausal status. Conclusion LINC00963 and LNC01089, as the two potential tumor suppressors of BC, could regulate the FOXO signaling pathway through direct interaction with IGF1 mRNA. miR-1244-5p also might have a critical role in FOXO regulation through suppression of IGF1 and two mentioned lncRNAs.

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LINC01089 blocks malignant progression of thyroid cancer by binding miR-27b-3p to enhance the FBLN5 protein level

Cited by 4 publications

References 36 publications

LncRNA PGM5-AS1 inhibits non-small cell lung cancer progression by targeting miRNA-423-5p/SLIT2 axis

LncRNA PGM5-AS1 inhibits non-small cell lung cancer progression by targeting miRNA-423-5p/SLIT2 axis

Reveal the potential molecular mechanism of circRNA regulating immune-related mRNA through sponge miRNA in the occurrence and immune regulation of papillary thyroid cancer

LNC01089-LINC00963/miR-1244-5p/IGF1 ceRNA axis might regulate FOXO signaling pathway in breast cancer patients: a biomarker discovery investigation

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